Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.     

Documenting hurricane impacts on coral reefs using two-dimensional video-mosaic technology

Four hurricanes impacted the reefs of Florida in 2005. In this study, we evaluate the combined impacts of hurricanes Dennis, Katrina, Rita, and Wilma on a population of Acropora palmata using a newly developed video‐mosaic methodology that provides a high‐resolution, spatially accurate landscape view of the reef benthos. Storm damage to A. palmata was surprisingly limited; only 2 out of 19 colonies were removed from the study plot at Molasses Reef. The net tissue losses for those colonies that remained were only 10% and mean diameter of colonies decreased slightly from 88.4 to 79.6 cm. In contrast, the damage to the reef framework was more severe, and a large section (6 m in diameter) was dislodged, overturned, and transported to the bottom of the reef spur. The data presented here show that two‐dimensional video‐mosaic technology is well‐suited to assess the impacts of physical disturbance on coral reefs and can be used to complement existing survey methodologies.     

Numerical modelling of the Sea of Azov's dynamics resulting from narrowing of the mouth of Taganrog Bay

The currents and surface level oscillations in the Sea of Azov ensuing from contraction of the mouth of Taganrog Bay are computed using the grid technique on the basis of a numerical solution to the system of quasi-linear, degenerate, parabolic, partial derivative equations (shallow-water equations). It is demonstrated what changes are to be expected in the dynamics of the Sea of Azov should this project be realized. The computations were carried out for typical wind directions.Translated by Vladimir A. Puchkin.     

A Zonal Pathway for NADW in the South Atlantic

Several large deployments of neutrally buoyant floats took place within the Antarctic Intermediate (AAIW), North Atlantic Deep Water (NADW), and the Antarctic Bottom Water (AABW) of the South Atlantic in the 1990s and a number of hydrographic sections were occupied as well. Here we use the spatially and temporally averaged velocities measured by these floats, combined with the hydrographic section data and various estimates of regional current transports from moored current meter arrays, to determine the circulation of the three major subthermocline water masses in a zonal strip across the South Atlantic between the latitudes of 19°S and 30°S. We concentrate on this region because the historical literature suggests that it is where the Deep Western Boundary Current containing NADW bifurcates. In support of this notion, we find that a net of about 5 Sv. of the 15–20 Sv that crosses 19°S does continue zonally eastward at least as far as the Mid-Atlantic Ridge. Once across the ridge it takes a circuit to the north along the ridge flanks before returning to the south in the eastern half of the Angola Basin. The data suggest that the NADW then continues on into the Indian Ocean. This scheme is discussed in the context of distributions of dissolved oxygen, silicate and salinity. In spite of the many float-years of data that were collected in the region a surprising result is that their impact on the computed solutions is quite modest. Although the focus is on the NADW we also discuss the circulation for the AAIW and AABW layers.     

Jason-1: Assessment of the System Performances

On 7 December 2001, Jason-1 was successfully launched by a Boeing Delta II rocket from the Vandenberg Air Force Base, California. The Jason-1 satellite will maintain the high accuracy altimeter service provided since 1992 by TOPEX/Poseidon (T/P), ensuring the continuity in observing and monitoring the Ocean Dynamics (intraseasonal to interannual changes, mean sea level, tides, etc.). Despite one-fourth the mass and power, the Jason-1 system has been designed to have basically the same performance as T/P, measuring sea surface topography at a centimetric level. This new CNES/NASA mission also provides near real-time data for sea state and ocean forecast. The first two months of the Jason-1 mission have been dedicated to the assessment of the overall system. The goals of this assessment phase were:

1. To assess the behavior of the spacecraft at the platform and payload levels (Jason-1 being the first program to call on the PROTEUS versatile multimission platform for Low and Medium Earth Orbit Missions developed in partnership between Alcatel Space and CNES);

2. To verify that platform performance requirements are met with respect to Jason-1 requirements;

3. To verify that payload instruments performance requirements evaluated at instrument level are met;

4. To assess the performance of the Jason-1 Ground System.

This article will display the main outputs of the assessment of the system. It will demonstrate that all the elements of the onboard and ground systems are within the specifications. Provision of data to the Jason-1 Science Working Team started at the end of March 2002. This is the goal of a six-month phase after closure of the initial assessment phase to derive the error budget of the system in terms of altimetry user products.     

Diet shifts of Caribbean grunts (Haemulidae) and snappers (Lutjanidae) and the relation with nursery-to-coral reef migrations

The spatial size distribution of grunts and snappers have previously indicated the separation of juveniles in nursery habitats from the adults on the coral reef. This implies life cycle migrations from nursery habitats (such as seagrass beds and mangroves) to the coral reef. If diet shifts are related to such migrations, then the diets of these fish must change before or around the fish size at which such migrations take place. A wide size range of juveniles of two grunt species (Haemulon sciurus and Haemulon flavolineatum) and of two snapper species (Lutjanus apodus and Ocyurus chrysurus) were caught in seagrass beds and mangroves, and their gut contents identified and quantified. Regression analysis between fish size and dietary importance of small crustaceans showed a negative relationship in all four species. Positive relations were found for H. sciurus, L. apodus and O. chrysurus between fish length and the dietary importance of decapods, and for L. apodusand O. chrysurus between fish length and prey fish importance. Critical changes in the fish diets with fish size were examined by application of a Canonical Correspondence Analysis (CCA). The CCA yielded three clusters of size-classes of fishes with similar diets, and application of a Mantel test showed that each of these clusters had significantly different diets, and that each cluster diet was significantly specialised. The size at which a fish species ‘switched’ from one cluster to another was compared with size-at-maturity data and with the typical size at which these species migrate from the nursery habitats to the coral reef. H. sciurus and H. flavolineatum may be prompted to migrate from the nursery habitats to coral reef habitats because of dietary changes, or because of the development of the gonads. For L. apodus and O. chrysurus, a dietary changeover forms a more likely explanation for nursery-to-reef migrations than does sexual maturation because these species reach maturity at sizes much larger than the maximum size of individuals found in nursery habitats. Although other factors may theoretically initiate or promote the migration patterns, the results of this study indicate that ontogenetic dietary changes may crucially influence the nursery-to-coral reef migrations of these reef fish species.