Estrogenic responses to xenobiotics in channel catfish (Ictalwus punctatus)

Several environmentally relevant contaminants are characterized as xenoestrogens by virtue of their ability to induce responses similar to 17β-estradiol (E2). There is concern that exposure to these xenoestrogens may result in endocrine and, thus, reproductive disruption. The objective of the present research was to determine whether xenobiotics known to elicit estrogenic responses in mammals were estrogenic in channel catfish, and if these compounds were capable of altering normal physiological response to E2. Sexually immature catfish were exposed by intraperitoneal injection to E2, suspected xenoestrogens or combination doses of E2 and xenoestrogen. Appearance of vitellogenin (Vg) in serum was used as the bioindicator of estrogenicity; Vg was measured by ELISA 7 days after injection. The ED₅₀ of E2 for the appearance of Vg in blood, 0.6 mg/kg, was used as the positive control. Synthetic estrogens, ethinylestradiol, mestranol and DES were effective in inducing Vg; the antiestrogen tamoxifen inhibited response to E2 when given in a combination dose. Treatment by both methoxychlor and p-nonylphenol resulted in Vg appearance in serum; the doses required were 300 times that of E2, and the vitellogenic response was less when compared to the E2-induced response. In addition, a combination dose of methoxychlor with E2 decreased the magnitude of the response to E2. Other mammalian xenoestrogens, o,p′-DDT, chlordecone, lindane and β-HCH, were not able to produce a vitellogenic response in this study.     

Benthic mineralization rates at two locations in the southern North Sea

Benthic oxygen uptake, sulphate reduction and benthic bacterial production were measured at two contrasting locations in the southern North Sea: the shallow and turbulent Broad Fourteens area in the Southern Bight, and the deeper Oyster Grounds, a deposition area, where thermohaline stratification occurs during summer. Oxygen uptake and sulphate reduction showed a clear seasonal pattern in the Broad Fourteens area, indicating a supply of carbon to the benthic system that is closely related to the standing stock of carbon in the water column. This close benthic-pelagic coupling is probably due to the influence of the tide in this part of the North Sea, which keeps the water column permanently mixed. At the Oyster Grounds, no seasonal pattern was observed. Peaks in oxygen uptake and sulphate reduction were found in winter. Irregularly occurring events, such as storms and fishery-related activities, are likely to affect the benthic mineralization patterns in this area. Annual benthic carbon mineralization rates estimated from oxygen uptake rates were 44 gC·m⁻² at the Broad Fourteens, and 131 gC·m⁻² at the Oyster Grounds, of which 26 and 28%, respectively, could be attributed to sulphate reduction (assuming an annual sulphide reoxidation rate of 100%). Although sulphate reduction rates in the southern North Sea are higher than previously suggested, aerobic respiration is the most important pathway for benthic carbon mineralization at the stations visited. Production rates of benthic bacterial carbon measured with labelled leucine were much higher than carbon mineralization rates based on oxygen uptake or sulphate reduction. This may either imply a very high bacterial carbon conversion efficiency, or point to shortcomings in the accuracy of the techniques. A critical evaluation of the techniques is recommended.     

Analytical investigations on loop-manoeuvre of underwater towed cable-array system

The paper reports on analytical case studies on the loop manoeuvre of an underwater towed cable-array system to give an insight into the tactical aspects of their naval applications. The analytical formulation and the solution procedure adopted for this three dimensional dynamic problem is also described. The case studies have been carried out for different loop radii, tow lengths, tow speeds and truncated loops. The resulting X,Y,Z co-ordinates of the towed cable-array and tow-point tensions have been computed. Based on these results, the tow-length to loop radii ratio, leading to cable-array system looping, has been found. The values of speed reduction and turning radius, causing dangerous relaxation in tow-point tension have been worked out. After exit from the loop, the timing required for realignment of the cable-array system with the ship's heading can be obtained from plots of the results. These timings help to plan ship manoeuvres to optimise reestablishment of stable listening conditions during underwater surveillance.     

Near-surface seismic facies at the Korea Strait shelf margin and trough region

High-resolution seismic profiles across the shelf margin and trough region of the Korea Strait reveal five shallow, near-surface facies units. These are relict coastal deposits, relict delta deposits, slumps and slides, and trough lag deposits. Most deposits represent a lowstand systems tract, formed during the last lowstand of sea level. Relict coastal deposits represent a linear sediment body along the present shelf margin at water depths of 120–150 m, whereas relict delta deposits occur on the gentle, southwestern slope of the trough at water depths of about 150–200 m. Slumps and slides are dominant at the base of slope in the central trough region. Sediments on the central trough floor were partly eroded and redistributed by strong currents, resulting in lag deposits.     

On the choice of data transformation for modelling time series of significant wave height

Autoregressive models have been shown to adequately model the time series of significant wave height. However, since this series exhibits a seasonal component and has a non-gaussian nature, it is necessary to transform the series before a model can be fit to the data. Two different transformations that have been used in earlier work are shown not to be appropriate for all types of applications. A third transformation is proposed here, which combines the better features of the two earlier ones and which is appropriate for simulation work. This is demonstrated with an example of a series from Figueira da Foz, a location of the Portuguese Coast.     

Surfactant components of marine organic matter as agents for biogeochemical fractionation and pollutant transport via marine aerosols

The role of surfactant organic matter in marine aerosol production has been studied under conditions in which there is a large coverage of whitecaps on the sea surface. To improve the knowledge of matter exchange and pollutant recycling from the sea surface into the atmosphere, a spray drop adsorption model (SDAM) was developed and the validity of the proposed model verified by the following experimental results: (1) an increase of surfactant matter on the sea surface during rough sea conditions (‘surface wave concentration'); (2) an (hyperbolic-like) increase of the enrichment ratio (ER) of surfactant fluorescent organic matter (SFOM), made up predominantly by humic substances (HS), as the particle size decrease; (3) a similar behaviour for elements with pollutant properties, and which are known to interact with HS and other surfactant materials, considered pollution tracers. An additional laboratory experiment, based on the adsorption model conditions, gives enrichment ratio greater than unity for K and Ca. The first results on marine aerosols trapped in marine clouds (at 1000 m above sea level and at 100 km from the coast) seem to further support the proposed model and its ability to predict the transition from saline to almost entirely organic particles for the smaller fractions of marine aerosols. The possible contribution of these particles to the recycling and to the long range transport of pollutants via marine aerosols has been considered.     

Determination of water component age in ocean models: application to the fate of North Atlantic Deep Water

A method allowing the calculation of both concentration and age of an individual water component is used to examine the penetration and fate of North Atlantic Deep Water (NADW) in a global ocean model. The method is consistent with the recent theory of water component age by Delhez et al. Its application in ocean models is straightforward and involves specification of two ideal tracers, and its efficacy is verified here via comparison with water component ages obtained by a second method, involving the time history of a single ideal tracer, and whose application is rather more restricted. Age estimates by the two methods are compared in the case of an interior ocean source region (suitable for marking the model's NADW, and forming the main focus of the study) and in the case of a ocean surface source region (featuring high density surface water in the far Northern Atlantic). The concentration and age of NADW are determined for two versions of the model, differing only in the inclusion or exclusion of isoneutral diffusion. The age and, especially, the concentration of NADW in the deep Southern, Indian and Pacific Oceans are significantly lower in the version with isoneutral diffusion. Both versions indicate that most of the NADW ultimately reaches the surface in the model Southern Ocean.     

Prediction of hydrodynamic forces on submarine pipelines using an improved Wake II Model

The hydrodynamic force model for prediction of forces on submarine pipelines as described includes flow history effect (wake effects) and time dependence in the force coefficients. The wake velocity correction is derived by using a closed-form solution to the linearized Navier–Stokes equations for oscillatory flow. This is achieved by assuming that the eddy viscosity in the wake is only time dependent and of a harmonic sinusoidal form. The forces predicted by the new Wake (Wake II) Model have been compared to Exxon Production Research Company Wake Model in terms of time histories (force shape) and magnitudes of peak forces. Overall, the model predictions by the Wake II Model are satisfactory and represent a substantial improvement over the predictions of the conventional models. The conventional force models representing adaptations of Morison's equation with ambient velocity and constant coefficients give predictions that are in poor agreement with the measurements especially for the lift force component. The Wake II Force Model can be used for submarine pipeline on-bottom stability design calculations for regular waves with various pipe diameters.     

Bathymetry,substrate and fishing areas of Southeast Atlantic high-seas seamounts

Most of the Southeast Atlantic Ocean is abyssal, and global bathymetries suggest that only ～3.2% of the areas beyond national jurisdiction (ABNJ; also known as the high seas, as defined in the United Nations Convention on the Law of the Sea [UNCLOS]) are shallower than 2 500 m. This study mapped bathymetry and characterised substrates in selected seamount summit areas, including several that have been or may become fishing areas. The southernmost location, the Schmitt-Ott Seamount, has exposed volcanic bedrock with surrounding flats covered by thin biogenic sediments and/or coral rubble that appears ancient. At Wüst, Vema, Valdivia and Ewing seamounts the basaltic base appears to be overlain by coral caps and other coral substrates (sheets, rubble). Adjacent summit plains have biogenic sediments of varying thickness. Vema has a flat, roughly circular summit, <100 m deep, with the shallowest point being a 22-m-deep summit knoll; the upper slopes have ancient coral framework, but the summit has a mixture of coralline and volcanic rock and coarse sediments, including extensive areas with coralline algae and kelp forests. Valdivia Bank is a 230-m-deep, flat, rocky area (～11 × 5 km), protruding steeply from the extensive multi-summit Valdivia subarea of the Walvis Ridge. The distribution of past fisheries in the Convention Area of the South East Atlantic Fisheries Organisation (SEAFO) was considered in relation to the new information on bathymetry and substrate.