Transient biouptake flux and accumulation by microorganisms: The case of two types of sites with Langmuir adsorption

The uptake of a chemical species by an aquatic microorganism is modelled considering two kinds of sites where Langmuirian adsorption is followed by first order internalisation kinetics. Simpler models, such as only one internalisation route (while most of the adsorption takes place on non-internalising sites) or a linear isotherm for adsorption on one or both sites, become limiting cases of this double-Langmuirian model. The model considers the sites located on the spherical (or semi-spherical) surface of the organism, and takes diffusion from the medium into explicit account. The numerical solution for the internalisation flux shows a maximum. We provide an estimate for the time needed to reach a certain proximity to steady state. The transient solution confirms that the analytical expressions for the steady-state flux are usually valid and that the accumulated amounts reflect the impact of the short-time uptake. The Instantaneous Steady-State Approximation (ISSA), where an intercept of the linear regression of accumulated amount as a function of time is interpreted as an adsorbed amount, can be critically assessed with the transient numerical code for two cases: (i) when the total burden of metal on the cell is the input data and (ii) when an extraction procedure provides further information on the adsorbed and internalised amount.     

A community-wide intercomparison exercise for the determination of dissolved iron in seawater

The first large-scale international intercomparison of analytical methods for the determination of dissolved iron in seawater was carried out between October 2000 and December 2002. The exercise was conducted as a rigorously “blind” comparison of 7 analytical techniques by 24 international laboratories. The comparison was based on a large volume (700 L), filtered surface seawater sample collected from the South Atlantic Ocean (the “IRONAGES” sample), which was acidified, mixed and bottled at sea. Two 1-L sample bottles were sent to each participant. Integrity and blindness were achieved by having the experiment designed and carried out by a small team, and overseen by an independent data manager. Storage, homogeneity and time-series stability experiments conducted over 2.5 years showed that inter-bottle variability of the IRONAGES sample was good (< 7%), although there was a decrease in iron concentration in the bottles over time (0.8–0.5 nM) before a stable value was observed. This raises questions over the suitability of sample acidification and storage.     

On non-causal impulse response functions related to propagating water waves

Particular attention is drawn to the non-causality of two impulse response functions, one of which relates the excitation force on an immersed body to the incident wave elevation at the body's reference position, while the other relates the incident wave elevations at two different positions along the line of wave propagation. An explanation is proposed for the non-causality of the impulse response functions, in spite of the fact wave propagation is a causal process. An indication is given of how far ‘upstream’ the incident wave elevation should be measured in order to be able to know, with reasonable accuracy, the current heave excitation force on a floating truncated cylinder with vertical axis, given current and past wave-elevation measurements. This provides a method for wave prediction, which is required for optimum control of the oscillation of the immersed body.     

A residual analysis method for quantitative comparison of spatial data from the Iceland-Faeroes Front

A spatial statistical method has been developed from the well-known Kriging technique in geostatistics, as a way of providing quantitative comparison between a pair of spatial data sets, and a measure for such a comparison. This residual analysis method is applied to oceanographic data in order to compare Iceland-Faeroes Front (IFF) model predictions against appropriate field observations, with an aim to assess the IFF model performance and its prediction accuracy. The method is also used to evaluate the model-generated dynamical variability within the model predictions, as well as the natural variability within the frontal observations. From the results, it has been found that the IFF model is highly robust, and gives better predictions at depth 150–350 m than elsewhere. Within such a depth (i.e. 250–350 m), there is also evidence to suggest that the main frontal region is most active above the IF Ridge. The natural variability obtained from the observations appears to be comparable to the model-generated dynamical variability after 20 days of integration, indicating a certain degree of accuracy in the model predictions. The method reported in this paper could also be extended for further use in model data assimilation. Thus, the work not only demonstrates how spatial statistics can be applied to oceanographic data, but also opens up new statistical tools for data handling in ocean modelling.     

Environmental overview of Eckernförde Bay,northern Germany

This environmental overview of Eckernförde Bay (northern Germany) summarizes the results of previous studies relevant to the Office of Naval Research's Coastal Benthic Boundary Layer (CBBL) Baltic field exercise conducted during 1993–1994. Significant environmental characteristics include the following: (1) surface sediment distribution is related to water depth, dictated primarily by hydrodynamic reworking of older glacial deposits; (2) the origin and characteristics of small-scale sedimentary structures depend on storm-generated waves and currents; (3) the proximity of the sea surface and sediment —water interface results in a pelagic—benthic coupling that drives biogeochemical processes and produces organicrich, acoustically turbid sediments; and (4) the bay floor is complicated topographically by pockmarks and manmade sedimentary structures.     

Geophysiology and Parahistology of the Interactions of Organisms with the Environment*

Abstract. Ecology and Global Ecology (GE) are terms by which the relations between the organism (or living matter as a whole) and the environment (or Earth as a whole) have been treated for almost a century. Geophysiology and Parahistology (PH) are terms slowly replacing older scientific thoughts jointly with an increasing number of modifications and alterations of the Darwinian Evolution (DE) concept. Somehow Geophysiology and Parahistology seem to describe evolution in a non-Darwinian domain. According to V.I. Vernadsky (1929,1930,1988) - the great Russian naturalist and biogeochemist - the biogeochemical processes on Earth are controlled by the force of living matter rather than by species associations developing in and with individual ecosystems as expressed by darwinian evolutionary terms. He also claimed that Goethe was incorrectly regarded as a predecessor of DE by some authors (including Darwin) and that “Natur” (nature) and “Lebendige Natur” (the totality of creatures) are two very different things for Goethe. Detailed analyses of microbial mat systems in the German Wadden Sea and in artificial hypersaline WInogradsky columns have shown that the totality of creatures and matter around them i.e., the “lebendige Natur”sensu Goethe or “living matter”sensu Vernadsky of such environments control to a considerable extent the structure, stability. and (geo-)morphology of sediments and thereby the geological structure of the living Earth. These structures do not follow the rules of sedimentation formulated by the laws of Stokes They represent growth structures (Aufwuchs), whose physics and dynamics are controlled by complex fractal systems. The factors controlling the ultimate shape and stabilisation potential of the eventually resulting rocks and fossils are comparable to tissue development in macroorganisms. Also, certain microbial associations in the sub-recent and in the fossil record may be compared to metazoan tissues. Chemical gradients in the sedimentary column, regulated by the interplay of living matter and sluggish (slow-reactive to non-reactive) compounds, combine to create a pattern of porosity and structure of the resulting deposits that clearly indicates microbial influences and especially those of extracellular polymeric substances on the morphology and texture. The combined effects of microbiota or living matter on the sedimentary record are described as parahistology of sediments in analogy of the histology of tissue on a geological scale. This conceptual living tissue made up of microbially generated rocks and ore deposits cycled through metabolic processes and forced into tissue-like structures by microbial biofilms and mats may extend down to the upper mantle of Earth and far up into the stratosphere when Earth is regarded as a living entity over geological periods. We may have to conceive Earth as a living specimen, which is breathing at a frequency of thousands of years instead of the normal physiological breathing rate of man or an insect. Macroorganisms in all terrestrial systems represent the transport and logistic media, which, however, utterly depend on myriads of intra-, inter-, and extracellular microbial symbiotic partners.