用纤维素乙酸平板电泳测定中国卤虫的同功酶变异(英文)

本文报导了应用纤维素乙酸平板电泳方法,测定采于中国山东省东方红盐场卤虫(Artemia)的同功酶。经测定,8种同功酶其中有6种是多态的,即:苹果酸脱氢酶(MDH)、异柠檬酸酶(IDH)、谷氨酸草醋酸转移酶(GOT)、葡糖-6-磷酸盐脱氢酶(D6PHD)、6-磷酸萄糖脱氢酶(6PGDH)和磷酸葡糖异构酶(PGI)。本文所提供的研究方法,不仅适用于基础研究,而且在水产养殖业上有助于对卤虫的筛选和确证。     

Regulation of Tilapia metallothionein gene expression by heavy metal ions

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.     

The circulation of the Indian Ocean at 32°S

Using inverse methods a circulation for a new section along 32°S in the Indian Ocean is derived with a maximum in the overturning stream function (or deep overturning) of 10.3 Sv at 3310 m. Shipboard and Lowered Acoustic Doppler Current Profiler (ADCP) data are used to inform the choice of reference level velocity for the initial geostrophic field. Our preferred solution includes a silicate constraint (−312 ± 380 kmol s⁻¹) consistent with an Indonesian throughflow of 12 Sv. The overturning changes from 12.3 Sv at 3270 m when the silicate constraint is omitted to 10.3 Sv when it is included. The deep overturning varies by only ±0.7 Sv as the silicate constraint varies from +68 to −692 kmol s⁻¹, and by ±0.3 Sv as the net flux across the section, driven by the Indonesian throughflow, varies from −7 to −17 Sv with an appropriately scaled silicate flux constraint. Thus, the overturning is insensitive to the size of the Indonesian throughflow and silicate constraint within their apriori uncertainties. We find that the use of the ADCP data adds significant detail to the horizontal circulation. These resolved circulations include the Agulhas Undercurrent, deep cyclonic gyres and deep fronts, features evidenced by long term integrators of the flow such as current meter and float measurements as well as water properties.     

Balancing deformation in NW Borneo: Quantifying plate-scale vs. gravitational tectonics in a delta and deepwater fold-thrust belt system

Recent GPS measurements demonstrate that NW Borneo undergoes 4–6 mm of plate-scale shortening a year, which is not accommodated by plate-scale structures. The only geological structure in NW Borneo described to accommodate on-going shortening is the Baram Delta System located on the outer shelf to basin floor. Delta toe fold-thrust belts are commonly thought to be caused by margin-normal compressional stresses generated by margin-parallel upslope gravitational extension.     

The changing role of ecohydrological science in guiding environmental flows

Abstract

The term “environmental flows” is now widely used to reflect the hydrological regime required to sustain freshwater and estuarine ecosystems, and the human livelihoods and well-being that depend on them. The definition suggests a central role for ecohydrological science to help determine a required flow regime for a target ecosystem condition. Indeed, many countries have established laws and policies to implement environmental flows with the expectation that science can deliver the answers. This article provides an overview of recent developments and applications of environmental flows on six continents to explore the changing role of ecohydrological sciences, recognizing its limitations and the emerging needs of society, water resource managers and policy makers. Science has responded with new methods to link hydrology to ecosystem status, but these have also raised fundamental questions that go beyond ecohydrology, such as who decides on the target condition of the ecosystem? Some environmental flow methods are based on the natural flow paradigm, which assumes the desired regime is the natural “unmodified” condition. However, this may be unrealistic where flow regimes have been altered for many centuries and are likely to change with future climate change. Ecosystems are dynamic, so the adoption of environmental flows needs to have a similar dynamic basis. Furthermore, methodological developments have been made in two directions: first, broad-scale hydrological analysis of flow regimes (assuming ecological relevance of hydrograph components) and, second, analysis of ecological impacts of more than one stressor (e.g. flow, morphology, water quality). All methods retain a degree of uncertainty, which translates into risks, and raises questions regarding trust between scientists and the public. Communication between scientists, social scientists, practitioners, policy makers and the public is thus becoming as important as the quality of the science.

Editor Z.W. Kundzewicz

Citation Acreman, M.C., Overton, I.C., King, J., Wood, P., Cowx, I.G., Dunbar, M.J., Kendy, E., and Young, W., 2014. The changing role of ecohydrological science in guiding environmental flows. Hydrological Sciences Journal, 59 (3–4), 433–450     

Biological structures as a source of habitat heterogeneity and biodiversity on the deep ocean margins

Biological structures exert a major influence on species diversity at both local and regional scales on deep continental margins. Some organisms use other species as substrates for attachment, shelter, feeding or parasitism, but there may also be mutual benefits from the association. Here, we highlight the structural attributes and biotic effects of the habitats that corals, sea pens, sponges and xenophyophores offer other organisms. The environmental setting of the biological structures influences their species composition. The importance of benthic species as substrates seems to increase with depth as the complexity of the surrounding geological substrate and food supply decline. There are marked differences in the degree of mutualistic relationships between habitat-forming taxa. This is especially evident for scleractinian corals, which have high numbers of facultative associates (commensals) and few obligate associates (mutualists), and gorgonians, with their few commensals and many obligate associates. Size, flexibility and architectural complexity of the habitat-forming organism are positively related to species diversity for both sessile and mobile species. This is mainly evident for commensal species sharing a facultative relationship with their host. Habitat complexity is enhanced by the architecture of biological structures, as well as by biological interactions. Colony morphology has a great influence on feeding efficiency for suspension feeders. Suspension feeding, habitat-forming organisms modify the environment to optimize their food uptake. This environmental advantage is also passed on to associated filter-feeding species. These effects are poorly understood but represent key points for understanding ecosystems and biodiversity on continental margins. In this paper we explore the contributions of organisms and the biotic structures they create (rather than physical modifications) to habitat heterogeneity and diversity on the deep continental margins.     

Recent Rapid Regional Climate Warming on the Antarctic Peninsula

The Intergovernmental Panel on Climate Change (IPCC) confirmed that mean global warming was 0.6 ± 0.2 °C during the 20th century and cited anthropogenic increases in greenhouse gases as the likely cause of temperature rise in the last 50 years. But this mean value conceals the substantial complexity of observed climate change, which is seasonally- and diurnally-biased, decadally-variable and geographically patchy. In particular, over the last 50 years three high-latitude areas have undergone recent rapid regional (RRR) warming, which was substantially more rapid than the global mean. However, each RRR warming occupies a different climatic regime and may have an entirely different underlying cause. We discuss the significance of RRR warming in one area, the Antarctic Peninsula. Here warming was much more rapid than in the rest of Antarctica where it was not significantly different to the global mean. We highlight climate proxies that appear to show that RRR warming on the Antarctic Peninsula is unprecedented over the last two millennia, and so unlikely to be a natural mode of variability. So while the station records do not indicate a ubiquitous polar amplification of global warming, the RRR warming on the Antarctic Peninsula might be a regional amplification of such warming. This, however, remains unproven since we cannot yet be sure what mechanism leads to such an amplification. We discuss several possible candidate mechanisms: changing oceanographic or changing atmospheric circulation, or a regional air-sea-ice feedback amplifying greenhouse warming. We can show that atmospheric warming and reduction in sea-ice duration coincide in a small area on the west of the Antarctic Peninsula, but here we cannot yet distinguish cause and effect. Thus for the present we cannot determine which process is the probable cause of RRR warming on the Antarctic Peninsula and until the mechanism initiating and sustaining the RRR warming is understood, and is convincingly reproduced in climate models, we lack a sound basis for predicting climate change in this region over the coming century.     

Microearthquake seismicity and active tectonics of northwestern Greece

We carried out a microearthquake survey lasting for six weeks in northwest Greece using 18 portable seismograph stations to examine a region in which normal and thrust faulting have been reported in close proximity to one another. With this array we located 148 events and determined fault plane solutions for eight events using only rays radiated upwards. The seismicity of the region is diffuse with events extending to depths of nearly 30 km, and there is a minimum in activity near a depth of 15 km. The fault plane solutions exhibit a wide spectrum of fault types and orientations and are not consistent with simple zones of shortening or extension. Neither tractions applied to the edges or bottom of the region nor deviatoric stresses that compensate for lateral variations in crustal thickness can account for the variety of fault plane solutions. We think that the complicated behavior is a manifestation of inhomogeneous deformation due, at least in part, to a pre-existing complicated juxtaposition of structures and formations.