Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

In this study, we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay. Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h. The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%, respectively, and the half-inhibitory concentration of PE (IC₅₀) on P388 and BEL-7402 cells was 120 μg/mL and >200 μg/mL, respectively. Microscopic observation shows that the morphologic features of tumor cells treated with PE and 5-fluorouracil are markedly different from the normal control group. The inhibitory rate of fraction A₂ isolated from PE by sephadex LH-20 for BEL-7402 and P388 cells at the sample concentration of 70.42 μg/mL was 61.96±7.02% and 40.47±8.70%, respectively. The apoptosis peak for fraction A₂ was the most profound of all fractions used in the flow cytometry assay. The results indicate that the anti-proliferative of this algal extract is associated with the total phlorotannin content.     

Source Evolution and Its Relationship to Climate Change Since the Middle–Late Pleistocene in Laizhou Bay,China

Through the geochemical analysis of two hundred-meters cores KD4 and ZK3 from Laizhou Bay,in this study,we determined the distribution law and controlling factors of the geochemical elements.We analyzed 24 elements with respect to their R factors and major principal components,which were combined with the source discrimination functions DFCr/Th and DFCa/Al to obtain the sediment source index and its variation with depth for this area.A comparison of the changes in climate indicators suggests a clear correlation between the source and climate changes.The results show that the Yellow River and surrounding short-term rivers are the main sediment sources in this area.The PC3 of the KD4 core and PC2 of the ZK3 core(e.g.,CaO,MnO,SiO2)exhibit significant variations and reflect the relative contributions of Yellow River sources.The deposition process can be divided into six stages:in Phase I(MIS 5c–MIS 5a),the Yellow River formed,and the composition of the Yellow River had a greater influence on the sedimentary composition of the study area.In Phase II(MIS 5a–MIS 3),the sediment sources of the Yellow River and the short-term streams in this area were wavering,with the sediments derived from short-term rivers playing a more important role.In Phase III(MIS 3),with a sharp drop in temperature,the study area was in the process of retreat,and the sediment source changed from the Yellow River to short-term rivers,after which the Yellow River source material remained the main sediment source for the region.A similar process occurred three more times in Phase IV(MIS 3–MIS 2),Phase V(MIS 2–MIS 1),and Phase VI(MIS 1).With changes in climate,especially during alternating sea-land phases,the sediment source varied in marine-terrestrial-marine phases,and the changes are observed as Yellow River source-surrounding provenance-Yellow River source.However,this process of change is not synchronized with the sea-land strata alternation.     

Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice

Excessive alcohol consumption leads to liver disease. Extensive evidence suggests that C-phycocyanin (C-PC), a chromophore phycocyanobilin derived from Spirulina platensis, exerts protective effects against chemical-induced organ damage. In this study, we investigated whether C-PC could protect against ethanol-induced acute liver injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHOL), low-density lipoprotein (LDL), liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD) content were measured, and pathological examination of liver sections were examined. C-PC showed obvious inhibitory effects on serum ALT, AST, TG, CHOL, LDL and MDA, and SOD content significantly increased in the liver. The structure of hepatic lobules was clear, liver sinus returned to normal, and liver cell cords were arranged in neat rows. Cloudiness, swelling, inflammatory cell infiltration and spotty necrosis of liver cells were significantly reduced. Therefore, C-PC can significantly protect against ethanol-induced acute liver injury.     

Difference in the influence of Indo-Pacific Ocean heat content on South Asian Summer Monsoon intensity before and after 1976/1977

Monthly ocean temperature from ORAS4 datasets and atmospheric data from NCEP/NCAR Reanalysis I/II were used to analyze the relationship between the intensity of the South Asian summer monsoon(SASM) and upper ocean heat content(HC) in the tropical Indo-Pacific Ocean.The monsoon was differentiated into a Southwest Asian Summer Monsoon(SWASM)(2.5°–20°N,35°–70°E) and Southeast Asian Summer Monsoon(SEASM)(2.5°–20°N,70°–110°E).Results show that before the 1976/77 climate shift,the SWASM was strongly related to HC in the southern Indian Ocean and tropical Pacific Ocean.The southern Indian Ocean affected SWASM by altering the pressure gradient between southern Africa and the northern Indian Ocean and by enhancing the Somali cross-equatorial flow.The tropical Pacific impacted the SWASM through the remote forcing of ENSO.After the 1976/77 shift,there was a close relationship between equatorial central Pacific HC and the SEASM.However,before that shift,their relationship was weak.     

光及补料条件对室内管道光反应器中三角褐指藻生长和岩藻黄素积累的影响

【目的】制备富含岩藻黄素的三角褐指藻(Phaeodactylum tricornutum)生物质。【方法】采用470 L室内管道光生物反应器系统,研究自养培养下光源、调光策略、补料培养操作条件对于细胞生长、生物量浓度和岩藻黄素积累的影响。【结果】在采用LED灯、梯度提升照度及重复补料半连续培养模式下,三角褐指藻细胞密度、生物量和岩藻黄素产率最高分别可达3.16×10⁷mL^-1、1.64 g/L和1.90 mg/(L·d),分别是日光灯为光源条件下的2.77倍、3.09倍和2.38倍(P<0.01)。【结论】在管道光生物反应器中获得的自养培养条件,可强化三角褐指藻生物量和岩藻黄素积累,为三角褐指藻积累岩藻黄素的室内可控规模化技术开发提供指导。     

羌塘盆地查郎拉地区中、新生代地质构造特征

本文系统研究了羌塘盆地查郎拉地区中、新生代的构造特征,将整个研究区划分为五个构造单元。即那日－ 破曲褶皱带、江达－ 杜日断褶带、塞仁那来－ 巴陇复式向斜断褶带、气相错－ 查巴断陷带、查曲公玛－ 日昂巴山间盆地。从晚三叠世以来,图幅内经历了印支期、燕山期、喜山期构造运动,印支期主要是在图幅北部上三叠统地层中形成北东向褶皱,燕山运动早期本区构造活动最为强烈,形成了全区范围内的东西向褶皱和断层,其中尤以南部塞仁那来－ 巴陇复式向斜断褶带最为强烈,构造最为紧密,燕山晚期,以断陷构造为主,形成中部的查曲公玛－ 日昂巴山间盆地和南部的气相错－ 查巴断陷带,喜山期主要是形成一些新构造活动。     

ESTIMATION OF SOUNDING ABILITY OF A BRILLOUIN LIDAR IN THE EAST CHINA SEA

Vertical profiles of sound speed in the sea can be measured by using laser excited Brillouin scattering. In this paper the dependence of the accuracy of sound speed measurement on the accuracy of the Brillouin shift measurement is analyzed. We calculated the maximum detecting depths of sound speed to an accuracy of 1 m/s by lidar with different laser pulse energy, platform altitude, telescope aperture and lidar effective attenuation coefficient. The estimation of sounding ability in the East China Sea is made in some stations. These data can be used in the design of Brillouin Lidar for the China Sea.     

从永夏陈四楼煤矿水文地质特征看岩溶陷落柱并不存在

根据区域和矿区地质资料 ,结合岩溶陷落柱的形成地质条件进行认真的分析研究 ,得出了否定的认识 ,对正确认识永夏矿区的水文地质条件 ,有效地开展矿井防治水工作 ,正确指导生产有重要意义。     

The very low-frequency oscillation in tropical Pacific

The very low-frequency oscillation (VLFO) on interannual scale in the tropical Pacific is studied by analyzing the main components of the sea surface temperature, sea level pressure, cloudiness, humidity and the equivalent potential temperature over the tropical ocean surface. The nature of two components of VLFO and its propagation features are discussed from the view of air-sea interaction.     

Dynamics and relationships of Ca, Mg, Fe in litter, soil fauna and soil in Pinus koraiensis-broadleaf mixed forest

The Liangshui Natural Reserve in Heilongjiang Province of China was selected as the study area. The authors collected the samples of forest litter （Tilia amurensis, Fraxinus mandshurica, Pinus koraiensis, Acer mono, Betula costata, and mixed litter）, soil in humus horizon （0--5cm） and soil horizon （5-20cm）, and soil macrofauna （Oligochaeta, Geophiloporpha and Juliformia） from 2001 to 2002. The role of soil macrofauna in the material cycle was analyzed through comparing the macro-element contents among various parts of the subsystems and using enrichment index （El）. The results indicate that dynamic changes of various litters are very complicated. The contents of Fe in each kind of litter increase firstly, and then decrease in the study period. The changes of macro-element contents are greater in the broad-leaf litter than in the coniferous litter, and the mixed litter is in the middle level, but the differences among them are not significant. The contents of Mg and Fe in humus are higher than those in soil, but the contents of Ca in soil are higher than that in humus. The dynamic changes of macro-element contents in soil and soil fauna are not consistent with those in litter. The diplopod presented obvious enrichment of Ca and Mg （E1〉1）, but it does not significantly enrich Fe. Earthworm has a stronger enrichment ability of Fe than diplopod and scolopendra, but E1〈1. Soil fauna can make great influences on the material cycle of the subsystems.