Modeling rhamnolipids production by Pseudomonas aeruginosa from immiscible carbon source in a batch system

A mathematical model to predict the rhamnolipids production by Pseudomonas aeruginosa from oleic acid in a two phase liquid-liquid batch reaction system, was developed in this study. The model was based on two theoretical assumptions: 1) the convective oleic acid mass transfer is coupled to a bioreaction in the aqueous liquid bulk, and 2) the volume of the immiscible oleic acid drops and the saturation concentration at the interface are a function of rhamnolipids production. The model was able to accurately predict the experimental growth of the Pseudomonas aeruginosa strain, and the rhamnolipids production data with oleic acid as carbon source. This mathematical approach indicated a high correspondence between the saturation dimensionless profiles of oleic acid at the interface and the experimental profiles of surface tension difference. This modeling approach may constitute a useful tool in the design and scaling-up of bioreactors applied to the production of biosurfactants with immiscible carbon sources.     

Measurement of stress effects (scope for growth) and contaminant levels in mussels (Mytilus edulis) collected from the Irish Sea

The objective of this research was to quantify the impact of pollution along the coastlines of the Irish Sea. Pollution assessment was based on the combined measurement of scope for growth (SFG), and chemical contaminants in the tissues of mussels (Mytilus edulis) collected from 38 coastal sites around the Irish Sea during June-July in 1996 and 1997. On the UK mainland coast, the SFG showed a general trend with a significant decline in water quality in the Liverpool and Morecambe Bay region. High water quality was recorded along the west coast of Wales, as well as southwest England and northwest Scotland (clean reference sites outside the Irish Sea). Along the coast of Ireland there was a similar trend with reduced SFG within the Irish Sea region. SFG was generally low north of Duncannon and then improved north of Belfast. The poor water quality on both sides of the Irish Sea is consistent with the prevailing hydrodynamics and the spatial distribution of contaminants associated with urban/ industrial development. The decline in SFG of mussels on both sides of the Irish Sea was associated with a general increase in contaminant levels in the mussels. Certain contaminants, including PAHs, TBT, sigmaDDT, Dieldrin, gamma-HCH, PCBs, and a few of the metals (Cd, Se, Ag, Pb), showed elevated concentrations. Many of these contaminants were particularly elevated in the coastal margins of Liverpool Bay, Morecambe Bay and Dublin Bay. A quantitative toxicological interpretation (QTI) of the combined tissue residue chemistry and SFG measurements indicated that at the majority of coastal sites, c. 50 to > 80% of the observed decline in SFG was due to PAHs as a result of fossil fuel combustion and oil spills. TBT levels were highest at major ports and harbours, but these concentrations only made a minor contribution to the overall reduction in SFG. At no sites were individual metals accumulated to concentrations that could cause a significant effect on SFG. The study identified many sites where the observed reduction in SFG was far greater than predicted from the limited number of chemical contaminants analysed, thus indicating the presence of additional 'unknown toxicants'. Sewage (containing domestic, agricultural and industrial components) appears to be an important contributor to reduced SFG and linear alkylbenzenes (LABs) and As may provide suitable 'sewage markers'. There was a highly significant positive correlation between SFG and As (P < 0.001). This relationship may be due to reduced As uptake by algal food material and mussels at sites with elevated P04 concentrations (e.g. at sites with sewage inputs). Phosphate is a known competitive inhibitor of As accumulation, at least in algae. The results highlight that further research is required on 'sewage markers' in mussels. The SFG approach therefore provides a rapid, cost-effective and quantitative measure of pollution impact, as well as a means of identifying the causes through a QTI of tissue contaminants levels. It also serves to identify the presence of unidentified toxicants and areas that require further study.     

Past,present and future vegetation-cloud feedbacks in the Amazon Basin

To begin exploring the underlying mechanisms that couple vegetation to cloud formation processes, we derive the lifting condensation level (LCL) to estimate cumulus cloud base height. Using a fully coupled land–ocean–atmosphere general circulation model (HadCM3LC), we investigate Amazonian forest feedbacks on cloud formation over three geological periods; modern-day (a.d. 1970–1990), the last glacial maximum (LGM; 21 kya), and under a future climate scenario (IS92a; a.d. 2070–2090). Results indicate that for both past and future climate scenarios, LCL is higher relative to modern-day. Statistical analyses indicate that the 800 m increase in LCL during the LGM is related primarily to the drier atmosphere promoted by lower tropical sea surface temperatures. In contrast, the predicted 1,000 m increase in LCL in the future scenario is the result of a large increase in surface temperature and reduced vegetation cover.     

Degradation of native wheat straw lignin by Streptomyces viridosporus T7A

Lignin is one of the major contributing factors toward the recalcitrance of lignocellulosic biomass. Understanding the process of lignin degradation in natural biological processes will provide useful information to develop novel biomass conversion technologies. Functional group changes in the lignin entities during the process may contribute to the cellulose degradation (utilization) by the microorganisms. In this study, compositional and advanced Fourier transform infrared, pyrolysis gas chromatography/mass spectrometry and ¹³C cross polarization/magic angle spinning nuclear magnetic resonance analysis were performed to explore the mechanism of biodegradation of wheat straw by Streptomyces viridosporus T7A. The results indicated that S. viridosporus T7A removed lignin and hemicelluloses as indicated by the increased carbohydrate/lignin ratio. Significant modification of carbonyl and methoxyl groups in the complex lignin structure was also evident. Most importantly, the quantitative results showed that lignin degradation was featured by deduction of guaiacyl unit. The results provide new insight for understanding lignin degradation by bacteria.     

MoisturEC: A New R Program for Moisture Content Estimation from Electrical Conductivity Data

Noninvasive geophysical estimation of soil moisture has potential to improve understanding of flow in the unsaturated zone for problems involving agricultural management, aquifer recharge, and optimization of landfill design and operations. In principle, several geophysical techniques (e.g., electrical resistivity, electromagnetic induction, and nuclear magnetic resonance) offer insight into soil moisture, but data‐analysis tools are needed to “translate” geophysical results into estimates of soil moisture, consistent with (1) the uncertainty of this translation and (2) direct measurements of moisture. Although geostatistical frameworks exist for this purpose, straightforward and user‐friendly tools are required to fully capitalize on the potential of geophysical information for soil‐moisture estimation. Here, we present MoisturEC, a simple R program with a graphical user interface to convert measurements or images of electrical conductivity (EC) to soil moisture. Input includes EC values, point moisture estimates, and definition of either Archie parameters (based on experimental or literature values) or empirical data of moisture vs. EC. The program produces two‐ and three‐dimensional images of moisture based on available EC and direct measurements of moisture, interpolating between measurement locations using a Tikhonov regularization approach.     

Comparison of the LEW88516 and ALHA77005 martian meteorites: Similar but distinct

Abstract By mineral and bulk compositions, the Lewis Cliff (LEW) 88516 meteorite is quite similar to the ALHA77005 martian meteorite. These two meteorites are not paired because their mineral compositions are distinct, they were found 500 km apart in ice fields with different sources for meteorites, and their terrestrial residence ages are different. Minerals in LEW88516 include: olivine, pyroxenes (low- and high-Ca), and maskelynite (after plagioclase); and the minor minerals chromite, whitlockite, ilmenite, and pyrrhotite. Mineral grains in LEW88516 range up to a few mm. Texturally, the meteorite is complex, with regions of olivine and chromite poikilitically enclosed in pyroxene, regions of interstitial basaltic texture, and glass-rich (shock) veinlets. Olivine compositions range from Fo₆₄ to Fo₇₀, (avg. Fo₆₇), more ferroan and with more variation than in ALHA77005 (Fo₆₉ to Fo₇₃). Pyroxene compositions fall between En₇₇Wo₄ and En₆₅Wo₁₅ and in clusters near En₆₃Wo₉ and En₅₃Wo₃₃, on average more magnesian and with more variation than in ALHA77005. Shock features in LEW88516 range from weak deformation through complete melting. Bulk chemical analyses by modal recombination of electron microprobe analyses, instrumental neutron activation, and radiochemical neutron activation confirm that LEW88516 is more closely related to ALHA77005 than to other known martian meteorites. Key element abundance ratios are typical of martian meteorites, as is its non-chondritic rare earth pattern. Differences between the chemical compositions of LEW88516 and ALHA77005 are consistent with slight differences in the proportions of their constituent minerals and not from fundamental petrogenetic differences. Noble gas abundances in LEW88516, like those in ALHA77005, show modest excesses of ⁴⁰Ar and ¹²⁹Xe from trapped (shock-implanted) gas. As with other ALHA77005 and the shergottite martian meteorites (except EETA79001), noble gas isotope abundances in LEW88516 are consistent with exposure to cosmic rays for 2.5–3 Ma. The absence of substantial effects of shielding from cosmic rays suggest LEW88516 spent this time as an object no larger than a few cm in diameter.     

The Askervein hill project: A finite control volume prediction of three-dimensional flows over the hill

A three-dimensional nonlinear numerical model, that has been extensively used previously to predict environmental water flows, was applied to predict the flow over an isolated hill, Askervein. Predictions are reported for winds approaching the hill from 210 ° and 180 ° clockwise from north, both under almost neutral atmospheric conditions.The model predictions were compared with data collected during a major field study in 1983. From the comparisons it was concluded that the model predicts the mean flow variables with good accuracy. Larger discrepancies were found for quantities related to the turbulence, pointing to deficiencies in the turbulence model, and perhaps in some of the measurements.     

Influence of the saffman force,lift force,and electric force on sand grain transport in a wind–sand flow

Quasi-horizontal trajectories of salting sand grains were found using high-speed video-recording in the desertified territory of the Astrakhan region. The sizes and displacement velocities of the saltating sand grains were determined. A piecewise logarithmic approximation of the wind profile in a quasi-stationary wind–sand flow is suggested, which is consistent with the data of observations and modeling. It was established that, in the regime of stationary saltation, the wind profile in the lower saltation layer of the wind–sand flow depends only slightly on the wind profile variations in the upper saltation layer. The vertical profiles of the horizontal wind component gradient in a quasi-stationary wind–sand flow were calculated and plotted. It was shown using high-speed video recording of the trajectory of a sand grain with an approximate diameter of 95 μm that the weightlessness condition in the desertified territory of the Astrakhan region in a stationary wind–sand flow is satisfied at a height of approximately 0.15 mm. The electric parameters of a wind–sand flow, which can provide for compensation of the force of gravity by the electric force, were estimated. In particular, if the specific charge of a sand grain is 100 μC/kg, the force of gravity applied to the sand grain can be compensated by the electric force if the vertical component of the electric field in a wind–sand flow reaches approximately 100 kV/m. It was shown that the quasi-horizontal transport of sand grains in the lower millimeter saltation layer observed in the desertified territory can be explained by the joint action of the aerodynamic drag, the force of gravity, the Saffman force, the lift force, and the electric force.     

Mesoscale and macroscale aspects of the morning Urban Heat Island around Athens,Greece

Summary This paper examines the characteristics of the morning Urban Heat Island (UHI) in Athens basin, Greece. The study is performed by using and analyzing mesoscale and synoptic data covering the period 1990–2001. The UHI was estimated using the 0600 Local Time (LT) minimum temperature differences between rural and urban areas of the city. The analysis results in 7 UHI classes. A strong UHI was found for the 1/3 of days. The specific meteorological characteristics (surface and upper air, cooling rate) of each UHI class were revealed and examined. The spatial and temporal characteristics of the UHI were also identified. The UHI is largest on nights with clear skies and low relative humidity. In all seasons the UHI switches on rapidly in afternoon. During spring and summer, sea breeze commonly reduces and delays the UHI. Cases documenting the sensitivity and rapidity of changes of the UHI to changes in classes (cloud cover, wind) are also presented. The cooling rate is higher in the urban area under negative and lower under positive UHI conditions. Mesoscale and macroscale phenomena were examined during the different UHI classes through a weather type scheme. It was emerged that high UHI classes are associated with anticyclonic conditions or advection in the lower troposphere, while low UHI classes are associated with strong northeasterly winds. Anticyclonic conditions which frequently occur in spring and early summer, reduce or reverse the UHI to Urban Cooling Island.