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991.
The active lifetime of extracellular enzymes is a critical determinant of the effectiveness of enzyme production as a means for heterotrophic marine microbes to obtain organic substrates. Here, we report lifetimes of three classes of extracellular enzyme in Arctic seawater. We also investigated the relative importance of photochemical processes and particle-associated processes in inactivating extracellular enzymes. Enzyme inactivation in filtered seawater was slow, with apparent half-lives of enzyme activities on the order of hundreds of hours. The presence of particles (including cells) did not significantly change inactivation rates, suggesting that the long half-lives observed in filtered seawater were realistic for enzymes in unfiltered seawater. Phosphatase and leucine aminopeptidase were susceptible to photoinactivation, but only under high intensity UV-B and UV-C illumination; there was no evidence for increased inactivation rates under natural illumination at our study site in Ny Ålesund, Svalbard. Comparison of inactivation rates of commercially-obtained enzymes from non-marine sources with the extracellular enzymes naturally present in Arctic seawater suggests that the natural enzymes contain structural features that confer longer lifetimes, consistent with observations reported by others from a range of field sites that cell-free enzymes can contribute a substantial fraction of total hydrolytic activity in the water column. 相似文献
992.
Andrew M. Moore Hernan G. ArangoGregoire Broquet Chris EdwardsMilena Veneziani Brian PowellDave Foley James D. DoyleDan Costa Patrick Robinson 《Progress in Oceanography》2011,91(1):50-73
The Regional Ocean Modeling System (ROMS) 4-dimensional variational (4D-Var) data assimilation systems have been systematically applied to the mesoscale circulation environment of the California Current to demonstrate the performance and practical utility of the various components of ROMS 4D-Var. In particular, we present a comparison of three approaches to 4D-Var, namely: the primal formulation of the incremental strong constraint approach; the dual formulation “physical-space statistical analysis system”; and the dual formulation indirect representer approach. In agreement with theoretical considerations all three approaches converge to the same ocean circulation estimate when using the same observations and prior information. However, the rate of convergence of the dual formulation was found to be inferior to that of the primal formulation. Other aspects of the 4D-Var performance that relate to the use of multiple outer-loops, preconditioning, and the weak constraint are also explored. A systematic evaluation of the impact of the various components of the 4D-Var control vector (i.e. the initial conditions, surface forcing and open boundary conditions) is also presented. It is shown that correcting for uncertainties in the model initial conditions exerts the largest influence on the ability of the model to fit the available observations. Various important diagnostics of 4D-Var are also examined, including estimates of the posterior error, the information content of the observation array, and innovation-based consistency checks on the prior error assumptions. Using these diagnostic tools, we find that more than 90% of the observations assimilated into the model provide redundant information. This is a symptom of the large percentage of satellite data that are used and to some extent the nature of the data processing employed. This is the second in a series of three papers describing the ROMS 4D-Var systems. 相似文献
993.
Numerical studies of methane production from Class 1 gas hydrate accumulations enhanced with carbon dioxide injection 总被引:2,自引:0,他引:2
Class 1 gas hydrate accumulations are characterized by a permeable hydrate-bearing interval overlying a permeable interval with mobile gas, sandwiched between two impermeable intervals. Depressurization-induced dissociation is currently the favored technology for producing gas from Class 1 gas hydrate accumulations. The depressurization production technology requires heat transfer from the surrounding environment to sustain dissociation as the temperature drops toward the hydrate equilibrium point and leaves the reservoir void of gas hydrate. Production of gas hydrate accumulations by exchanging carbon dioxide with methane in the clathrate structure has been demonstrated in laboratory experiments and proposed as a field-scale technology. The carbon dioxide exchange technology has the potential for yielding higher production rates and mechanically stabilizing the reservoir by maintaining hydrate saturations. We used numerical simulation to investigate the advantages and disadvantages of using carbon dioxide injection to enhance the production of methane from Class 1 gas hydrate accumulations. Numerical simulations in this study were primarily concerned with the mechanisms and approaches of carbon dioxide injection to investigate whether methane production could be enhanced through this approach. To avoid excessive simulation execution times, a five-spot well pattern with a 500-m well spacing was approximated using a two-dimensional domain having well boundaries on the vertical sides and impermeable boundaries on the horizontal sides. Impermeable over- and under burden were included to account for heat transfer into the production interval. Simulation results indicate that low injection pressures can be used to reduce secondary hydrate formation and that direct contact of injected carbon dioxide with the methane hydrate present in the formation is limited due to bypass through the higher permeability gas zone. 相似文献
994.
Zhenghong Tang Samuel D. BrodyRuopu Li Courtney QuinnNan Zhao 《Ocean & Coastal Management》2011,54(5):415-426
Local jurisdictions play a critical role in climate change mitigation and adaptation. This study analyzes the theoretical framework of locally driven climate change actions and uses geographic information system (GIS) to map local jurisdictions’ climate change policy efforts in three Pacific states - California, Oregon, and Washington. The results of our study indicate statistically significant differences in geographic clusters and variations across jurisdictions. An Ordinary Least Squares (OLS) regression model was used to examine climate risk, emission stress, and socioeconomic context variables to detect influence on local climate change policy efforts. The explanatory results indicate that coastal proximity, population density, vehicles emission, and education variables significantly influence local jurisdictions’ climate change actions. The findings contribute to local organizational decision model research and can help local communities to develop more effective climate change policies. 相似文献
995.
This article examines visitors’ normative acceptance of encounters with boats at a marine protected area and the extent that the number of boats, size of boats, and size of boat on which individuals were traveling influences these encounter norms. Data were obtained from a survey of 439 people visiting Molokini Shoal Marine Life Conservation District in Hawai’i, and photographs depicting four levels of boat use and three proportions of boat size measured encounter norms. Number of boats most strongly influenced encounter norms, boat size was less influential, and the size of boats on which respondents were traveling had little influence. Visitors wanted fewer boats at this site and a majority would not accept encountering more than 15 or 16 boats at one time. When the impact of boat size was considered, they would not accept encountering more than 12 large boats to 17 small boats. Implications for future research and managing standards of quality and social carrying capacity indicators at this marine protected area are discussed. 相似文献
996.
S.D. Archer K. SafiA. Hall D.G. CummingsM. Harvey 《Deep Sea Research Part II: Topical Studies in Oceanography》2011,58(6):839-850
The impact of in situ iron fertilisation on the production of particulate dimethylsulphoniopropionate (DMSPp) and its breakdown product dimethyl sulphide (DMS) was monitored during the SOLAS air-sea gas exchange experiment (SAGE). The experiment was conducted in the high nitrate, low chlorophyll (HNLC) waters of the sub-Antarctic Southern Ocean (46.7°S 172.5°E) to the south-east of New Zealand, during March-April, 2004. In addition to monitoring net changes in the standing stocks of DMSPp and DMS, a series of dilution experiments were used to determine the DMSPp production and consumption rates in relation to increased iron availability. In contrast to previous experiments in the Southern Ocean, DMS concentrations decreased over the course of the 15-d iron-fertilisation experiment, from an integrated volume-specific concentration in the mixed layer on day 0 of 0.78 nM (measured values 0.65-0.91 nM) to 0.46 nM (measured values 0.42-0.47 nM) by day 15, in parallel with the surrounding waters. DMSPp, chlorophyll a and the abundance of photosynthetic picoeukaryotes exhibited indiscernible or only moderate increases in response to the raised iron availability, despite an obvious physiological response by the phytoplankton. High specific growth rates of DMSPp, equivalent to 0.8-1.2 doublings d−1, occurred at the simulated 60% light level of the dilution experiments. Despite the high production rates, DMSPp accumulation was suppressed in part by microzooplankton grazers who consumed between 61% d−1 and 126% d−1 of the DMSPp production. Temporal trends in the rates of production and consumption illustrated a close coupling between the DMSP-producing phytoplankton and their microzooplankton grazers. Similar grazing and production rates were observed for the eukaryotic picophytoplankton that dominated the phytoplankton biomass, partial evidence that picoeukaryotes contributed a substantial proportion of the DMSP synthesis. These rates for DMSPp and picoeukaryotes were considerably higher than for chlorophyll a, indicating higher cycling rates of the DMSP-producing taxa than for the bulk phytoplankton community. When compared to the total phytoplankton community, there was no evidence of selection against the DMSP-containing phytoplankton by the microzooplankton grazers; the opposite appeared to be the case. SAGE demonstrated that increased iron availability in the HNLC waters of the Southern Ocean does not invariably lead to enhanced DMS sea-air flux. The potential suppression of DMSPp accumulation by grazers needs to be taken into account in future attempts to elevate DMS emission through in situ iron fertilisation and in understanding the hypothesised link between levels of Aeolian iron deposition in the Southern Ocean, DMS emission and global albedo. 相似文献
997.
Ye. I. Polyakova Ye. A. Novichkova A. P. Lisitzin V. P. Shevchenko M. D. Kravchishina 《Oceanology》2016,56(2):289-300
Diatom algae, aquatic palynomorphs, and the grain-size of surface sediments from bays of the White Sea were investigated in a program dedicated to the study of marginal filters (MF) in the Severnaya Dvina, Onega, and Kem rivers. Three microalgal assemblages are established in surface sediments, which replace each other successively with distance from river mouths and are characterized by a gradual decrease in a share of freshwater species of diatoms and Chlorophyceae algae, significantly varying concentrations of marine diatoms and dinocysts due to changes in water salinity, grain-size composition of sediments, quantitative distribution of suspended particulate matter (SPM), and water productivity at different marginal filter stages. 相似文献
998.
Oceanology - The variability of sea ice in the Weddell Sea, along with melting of glaciers of the Antarctic Peninsula and ice shelves, determines the volumes of melt water entering the water area,... 相似文献
999.
Gubanova D. P. Chkhetiani O. G. Kuderina T. M. Iordanskii M. A. Obvintsev Y. I. Artamonova M. S. 《Izvestiya Atmospheric and Oceanic Physics》2018,54(8):777-793
Izvestiya, Atmospheric and Oceanic Physics - This paper summarizes the results of long-term (2004–2016) comprehensive experimental studies of microphysical parameters and the mass... 相似文献
1000.
Bondur V. G. Vorobyev V. E. Zamshin V. V. Serebryany A. N. Latushkin A. A. Li M. E. Martynov O. V. Hurchak A. P. Grinchenko D. V. 《Izvestiya Atmospheric and Oceanic Physics》2018,54(9):1008-1022
Izvestiya, Atmospheric and Oceanic Physics - The results of comprehensive monitoring of anthropogenic impact on some coastal water areas of the Black Sea are presented. Multispectral satellite... 相似文献