Seasonal variation of airborne fungi of the Tiantishan Grottoes and Western Xia Museum,Wuwei, China

In this study, a systematic survey of cultural airborne fungi was carried out in the occurrence environments of wall paintings that are preserved in the Tiantishan Grottoes and the Western Xia Museum, China. A bio-aerosol sampler was used for sampling in four seasons in 2016. Culture-dependent and -independent methods were taken to acquire airborne fungal concentration and purified strains; by the extraction of genomic DNA, amplification of fungal ITS rRNA gene region, sequencing, and phylogenetic analysis, thereafter the fungal community composition and distribution characteristics of different study sites were clarified. We disclosure the main environmental factors which may be responsible for dynamic changes of airborne fungi at the sampling sites. The concentration of cultural airborne fungi was in a range from 13 to 1,576 CFU/m³, no significant difference between the two sites at the Tiantishan Grottoes, with obvious characteristics of seasonal variation, in winter and spring were higher than in summer and autumn. Also, there was a significant difference in fungal concentration between the inside and outside of the Western Xia Museum, the outside of the museum was far more than the inside of the museum in the four seasons, particularly in the winter. Eight fungal genera were detected, including Cladosporium, Penicillium, Alternaria, and Filobasidium as the dominant groups. The airborne fungal community structures of the Tiantishan Grottoes show a distinct characteristic of seasonal variation and spatial distribution. Relative humidity, temperature and seasonal rainfall influence airborne fungal distribution. Some of the isolated strains have the potential to cause biodeterioration of ancient wall paintings. This study provides supporting information for the pre-warning conservation of cultural relics that are preserved at local sites and inside museums.     

国内价值链构建下地方产业集群升级机理研究

承接产业转移是广大中西部地区的重大发展机遇。在分析全球价值链(GVC)的基础上,立足于我国经济梯度,提出了构建国内价值链(NVC)的基本思路。根据劳动力、资源、市场和区位等不同要素,明确了国内价值链主要划分为生产者驱动价值链、购买者驱动价值链、原材料驱动价值链和混合驱动价值链4种形式,重点分析了在不同价值链下地方产业集群升级的重点和方向,提出了不同的地方产业集群升级模式和内在机理。     

南极普里兹湾附近海域表层海流特征分析

利用第29次南极科考期间在普里兹湾海区释放的8个Argos漂流浮标所获的总计662 d的数据,对普里兹湾及其邻近海域的表层海流特征进行了分析,并和以前的研究结果进行对比,结果表明:普里兹湾西部和北部表层海流基本呈现向西的流动,说明此地区的表层海流以沿南极大陆的逆时针沿岸流为主。普里兹湾东部海流呈现先向南再向西或先向南再向北的流动,普里兹湾西北部海流呈现向南的流动。8个浮标平均流速在0.02—0.20 m·s~(-1)之间,最大流速为1.57 m·s~(-1),流速1.0 m·s~(-1)的大流速区主要集中在普里兹湾、克洛斯角外侧、达恩利角外侧海域,流速1.5 m·s~(-1)的大流速区只出现在普里兹湾、克洛斯角外侧两处海域。     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

1961-2008年中国西南地区极端气候变化幅度的海拔效应(英文)

A total of 12 indices of temperature extremes and 11 indices of precipitation ex-tremes at 111 stations in southwestern China at altitudes of 285-4700 m were examined for the period 1961-2008. Significant correlations of temperature extremes and elevation in-cluded the trends of diurnal temperature range, frost days, ice days, cold night frequency and cold day frequency. Regional trends of growing season length, warm night frequency, coldest night and warmest night displayed a statistically significant positive correlation with altitude. These characteristics indicated the obvious warming with altitude. For precipitation extreme indices, only the trends of consecutive dry days, consecutive wet days, wet day precipitation and the number of heavy precipitation days had significant correlations with increasing alti-tude owing to the complex influence of atmospheric circulation. It also indicated the increased precipitation mainly at higher altitude areas, whereas the increase of extreme precipitation events mainly at lowers altitude. In addition, the clearly local influences are also crucial on climate extremes. The analysis revealed an enhanced sensitivity of climate extremes to ele-vation in southwestern China in the context of recent warming.     

环境规制视角下城市绿色创新时空演变及溢出效应

基于2005—2019年中国266个地级及以上城市的面板数据,采用SBM-DEA模型评价城市绿色创新水平,刻画城市绿色创新的空间演变格局,最后构建空间杜宾模型实证分析不同类型的环境规制视角下城市绿色创新的溢出效应。研究结果显示：(1)时序发展上,研究期内城市绿色创新呈现东部地区、中部地区、西部地区、东北地区依次减弱的态势,总体上表现为“双M”型波动上升趋势,未来具有较大的增长潜力;(2)空间演化上,城市绿色创新在空间分布格局上存在明显的区域异质性,整体表现为东部地区主导发展的特征;(3)城市绿色创新在正式环境规制视角下存在正向溢出效应,在非正式环境规制视角下未产生显著溢出效应。     

中国留学人员创业园分布及产业特征

以《中国留学人员创业年鉴》为基础数据,采用GIS空间分析技术,运用核密度估计法、L函数和最近邻层次聚类分析等方法,分析2006~2015年中国留学人员创业园空间分布规律以及园区产业集聚特征,结果表明：中国留学人员创业园集聚特征显著,而且随着时间的推移,其多中心趋势明显,集聚规模迅速增大,集聚强度呈“先升后降,但总体增大”趋势;主要集聚于长三角、京津冀和珠三角等地区,尤以前两者最显著,且三大集聚区的集聚强度与集聚规模差异明显,其中长三角留创园的集聚规模最大,京津冀集聚强度最高,而珠三角则比较复杂,其在很小空间尺度范围内服从集聚分布,而在较大空间尺度范围内则服从随机分布;基于不同主导产业的中国留学人员创业园依然集中于长三角、京津冀和珠三角等地区,其主导产业分布格局则差异显著,规律性不强。     

末次间冰期127 ka时期植被反馈增强东亚夏季风降水的数值模拟研究

黄土和石笋等古气候代用资料表明在末次间冰期间,东亚夏季风增强、降水增多。本研究利用地球系统模式EC-Earth模拟了末次间冰期127 ka时期的气候,通过和工业革命前的气候模拟控制试验做比对,分析了127 ka时期由于地球轨道参数变化导致的东亚夏季风的空间变化特征。我们利用了两种EC-Earth的模式配置,即"大气-陆面-海洋-海冰"耦合模式和"大气-陆面-海洋-海冰-动态植被"耦合模式,分别估算轨道强迫和植被反馈对东亚夏季风降水变化的贡献。数值模拟结果表明,地球轨道强迫导致的海陆热力差异使得东亚夏季风系统显著增强并北移西伸,中国中部及华北地区降水增多而东部沿海地区降水减少。耦合了动态植被模式的试验结果表明,127 ka时期温暖湿润的气候致使东亚地区植被增多,植被的蒸腾作用使得地表的感热和潜热通量显著增大,从而增强了局地水循环,使降水进一步增多。植被的反馈作用在原本温暖湿润的华南地区对降水的影响并不显著,但是对相对干旱的我国中部和华北地区降水有显著影响。数值试验结果表明轨道强迫和植被反馈的共同作用能使内陆的四川盆地到华北一带夏季降水增加约40%,其中30%的增加是由于轨道强迫作用,约10%是由于植被反馈。这个研究也提醒我们,要得到更加合理的对过去或未来气候变化的模拟结果,有必要使用耦合动态植被的气候系统模式。

     

广东房地产业发展现状与前景分析

房地产开发热潮令理论界，新闻界和广大民众所关注，本文试图对广东省房地产发展的现状、特点及其主要问题作全面的分析，并提出解决问题的对策和建议，以及对广东省房地产业的发展前景作尝试性的探讨。     

Analysis of city centrality based on entropy weight TOPSIS and population mobility: A case study of cities in the Yangtze River Economic Belt

Based on statistical data and population flow data for 2016,and using entropy weight TOPSIS and the obstacle degree model,the centrality of cities in the Yangtze River Economic Belt(YREB)together with the factors influencing centrality were measured.In addition,data for the population flow were used to analyze the relationships between cities and to verify centrality.The results showed that:(1)The pattern of centrality conforms closely to the pole-axis theory and the central geography theory.Two axes,corresponding to the Yangtze River and the Shanghai-Kunming railway line,interconnect cities of different classes.On the whole,the downstream cities have higher centrality,well-defined gradients and better development of city infrastructure compared with cities in the middle and upper reaches.(2)The economic scale and size of the population play a fundamental role in the centrality of cities,and other factors reflect differences due to different city classes.For most of the coastal cities or the capital cities in the central and western regions,factors that require long-term development such as industrial facilities,consumption,research and education provide the main competitive advantages.For cities that are lagging behind in development,transportation facilities,construction of infrastructure and fixed asset investment have become the main methods to achieve development and enhance competitiveness.(3)The mobility of city populations has a significant correlation with the centrality score,the correlation coefficients for the relationships between population mobility and centrality are all greater than 0.86(P<0.01).The population flow is mainly between high-class cities,or high-class and low-class cities,reflecting the high centrality and huge radiating effects of high-class cities.Furthermore,the cities in the YREB are closely linked to Guangdong and Beijing,reflecting the dominant economic status of Guangdong with its geographical proximity to the YREB and Beijing's enormous influence as the national political and cultural center,respectively.