我国海洋文化事业发展现状和建设研究

在对我国海洋博物馆、海洋意识教育基地、海洋文化节庆活动、海洋文化论坛和海洋文化教材等领域的基本情况进行调研和分析的基础上,文章概括我国海洋事业发展的总体情况、地域范围和开展形式以及发展趋势,提出目前存在的海洋文化宣教涉及面不广、理论研究不足和国际影响力尚待提升等问题;借鉴发达国家博物馆大众化、节假日法定化和教育社会化等海洋文化事业发展经验,提出全面普查收集信息、创新教育形式、丰富展现形式和扩大群体覆盖面等举措,以促进我国海洋文化事业繁荣发展。     

MALBAC技术扩增微生物群落基因组的效率评估

环境样品的低生物量是微生物宏基因组学研究面临的首要挑战,通过基因组扩增技术来满足高通量测序对DNA样品量的需求是最常用的解决策略。MALBAC(Multiple Annealing and Looping Based Amplification Cycles)基因组扩增试剂盒最初为扩增和研究哺乳动物的单细胞基因组而研发。本文中,我们通过人工构建的微生物群落来检测该试剂盒在微生物宏基因组扩增方面的效率和应用可行性。结果表明,每个标准反应中,10 pg的DNA模板量足以满足MALBAC试剂盒对样品扩增的需要。每个标准反应DNA模板用量为10和100 pg时,所扩增DNA样品的基因组覆盖度与原始未扩增样品表现出高度的一致性,证明MALBAC试剂盒扩增效果的高度稳定性和一致性。常用的GenomePlex全基因组扩增试剂盒使我们可以在每个标准反应DNA模板量为100 pg的条件下扩增获得足够的DNA样品,但是结果表明该参照试剂盒无法有效的实现对群落中低丰度细菌菌株基因组的线性扩增。对于MALBAC试剂盒和参照试剂盒而言,在扩增高GC含量的微生物物种基因组DNA方面效率低下。我们的实验结果表明MALBAC试剂盒在高效扩增环境样品宏基因组DNA方面的可行性,但对该试剂盒在扩增环境样品中高GC含量微生物物种方面的适用性存在疑虑。     

风浪流中半潜式风机系统动力响应特性研究

以三浮筒(Tri-floater)型半潜式风机系统为对象,采用时域方法,考虑垂荡板的黏性阻尼效应,研究系泊缆直径、长度、预张力和布置方式等参数对其在风浪流中动力响应特性的影响规律,提出了一种系泊参数优化方法。在此基础上,根据东海和南海三个不同水深区域百年一遇海洋环境条件,通过调整Tri-floater型浮式基础的吃水和垂荡板等参数,有效地提高了其运动响应周期,减小了其运动响应幅值。同时,根据风机浮式基础运动幅值的设计规范要求,针对三个不同水深区域特点,设计了Tri-floater型浮式基础的系泊系统。计算结果表明,改进后的Tri-floater型半潜式风机系统能满足百年一遇极端环境下的作业要求,适合于东海和南海海洋环境下海上风能资源的开发。     

海洋产业结构变动对海洋经济效率影响研究

产业结构变动对经济增长影响已经得到了现代经济增长理论的肯定,但是产业结构变动对经济增长效率的影响一直是学术界没有解决的问题。目前中国已经进入了中等收入国家,粗放式增长逐渐结束,集约式增长越来越受到重视。文章基于海洋产业,研究了海洋产业结构变动对经济增长效率的影响,将海洋产业结构变动具体量化,分为海洋产业结构高级化和合理化;其次运用海洋产业全要素生产率来衡量海洋经济增长效率,通过从海洋生产函数中剔除初级要素带来的海洋经济增长效率得出海洋产业全要素生产率;同时计算出海洋产业劳动和资本的生产效率;最后收集了1996—2015年全国沿海11个省、市、自治区的数据,运用混合面板数据模型来研究海洋产业结构高级化和合理化对海洋经济增长效率的影响。研究发现海洋产业结构合理化和高级化对海洋经济增长具有正面影响,对于未来发展海洋经济提出了提高海洋产业技术来提高海洋产业结构优化,从而使得海洋经济发展走上集约式增长之路。     

离线固相萃取螯合富集分离-ICP-MS测定海水中的稀土元素

通过测定条件优化、方法比对等实验建立了一种固相萃取小柱离线螯合富集分离电感耦合等离子体质谱仪测定海水中稀土元素的方法。海水样品通过调节p H后,进入VAC ELUT SPS24 Agilent圆形固相萃取装置,其主要基体物质的去除率高于97%;萃取富集的优化条件是海水样品p H 4.0~7.0,海水进入萃取柱速率2 m L/min,硝酸洗脱液浓度为1 mol/L;方法对稀土元素的加标回收率为83%~108%,14种稀土元素的检出限为0.057~0.613 ng/L,RSD10%;该方法与氢氧化铁共沉淀法富集稀土元素比对测定结果一致,方法具有准确度与精密度高、操作简便快速等优点,可用于海水样品中稀土元素的定量精确测量。     

中国东海济舟岛南部春季水华的影响要素研究

A soil circulation occurs in the south of Cheju Island in the spring. Nutrients and its influence on chlorophyll a(Chl a) around the circulations were studied from April 9 to May 6, 2007. Spring bloom with elevated concentrations of Chl a was observed during the investigation. High concentrations of phosphate, nitrate and silicate at 0.6, 12, and 8 mmol/m3, respectively, were detected. A low water temperature prevented the growth of phytoplankton. Chl a concentrations in the study area might be strongly associated with the high silicate concentration.     

GNSS-R海洋遥感监测技术综述

介绍了GNSS-R海洋遥感的发展过程、技术原理和理论模型,进一步研究了GNSS-R在海洋遥感各应用领域的主要研究内容,探讨了GNSS-R海洋测风、测高、海冰监测、溢油检测和移动目标探测的技术途径,概述了GNSS-R海洋遥感监测技术所涉及的反演理论和信息提取方法,并根据现有研究成果和海洋遥感的应用需要,提出今后需解决的关键技术和未来的发展方向。     

西沙群岛生物碳酸盐的成因和沉积:来自活体仙掌藻的证据

A recent island survey reveals that the Xuande Atoll and the Yongle Atoll in the Xisha Islands can be classified into one of two systems: the depleted atoll system and growth atoll system; the survey also indicates that the decreased area of several shoals is an unbearable burden for the Xisha Islands, of which the largest island area is 2.13 km2 and the minimum elevation is 1.4 m. According to a survey on the ecological characteristics of Halimeda in the Laolongtou breaker zone of Shidao Island in the Xisha Islands, the green and white living Halimeda are collected,the isotopic ages of 14 C contained in the Halimeda are shown to be 27 years and 55 years, respectively, and carbonate mainly occurs in five types, i.e., luster, segment, sand, sand grain, and marl in the formation. The Halimeda segments mainly provide the carbonate sediments of long-term biogenic deposits in the reef environment and the annual productivity per area is 60–100 g/m2; the characteristics of the microstructure of the Halimeda are analyzed, the aragonite raphide carbonate is deposited and enriched in the cortexes, medullas and cysts, and the Halimeda generally contain major elements such as C, O, Ca, Cl, Mg, K, Na, S and Al, and are rich in trace elements such as tellurium(Te), rhodium(Rh) and strontium. It is believed that the Halimeda grow slowly,including the biotic community of reef corals in the reef areas, thus they possess an environmental remediation capacity, but it takes much time to remedy the environment, and it is necessary to make the law to protect the diversity and vulnerability of the Xisha marine ecology, the ecology of the reef community and the island environment in a scientific way. As indicated in the survey, under the background of global warming and sea-level rise, the discovery of large amounts of Halimeda in the Laolongtou sea area is significant for the natural increase of the depleted atoll system of the Xuande Atoll, while the Halimeda segments represent the primary form of the fossil Halimeda, of which the species can be identified and preserved in great numbers under geological conditions. The Miocene was discovered in large amounts in the Xichen-1 well, therefore the study on the characteristics and mechanism of Halimeda carbonate sediments plays a pivotal role in the formation and construction of organic reefs in the South China Sea as well as oil and gas exploration.     

厦门近岸水域海洋附着细菌的群落结构及其动态变化

为研究海洋附着细菌的群落结构及动态变化,在厦门近岸海区进行挂板实验.将无菌玻璃板浸没于海水中,连续放置14 d.分别于放置1 h和7、14 d后取玻璃板上附着生物样品.用细菌通用引物构建16S rRNA基因克隆文库,每个克隆文库随机挑选约40个克隆子测序,序列同源性分析和系统进化分析结果表明,所有的克隆子可分为六大类群:γ-变形菌纲(γ-Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、α-变形菌纲(α-Proteobacteria)、蓝细菌门(Cyanobacteria)和真核硅藻类叶绿体,各类群分别占42.0%、4.5%、2.2%、2.2%、1.1%和45.0%.γ-变形菌纲变形斑沙雷氏菌(Serratia proteamaculans)为优势附着细菌,占测序克隆子的31.5%.这类细菌在1 h样品中的比例超过一半,说明变形斑沙雷氏菌在生物膜形成初期发挥着重要作用.随着挂板时间延长,检测到的细菌类群有所增加:附着7 d后检测到拟杆菌门细菌,附着14 d后检测到厚壁菌门细菌.γ-变形菌纲细菌所占比例随挂板时间的延长而逐渐降低,从挂板1 h的81%降至7 d的21%,14 d的18%.另外,在各阶段的附着样品中,都检测到较多的真核克隆子序列,约占16%～64%.本研究为进一步阐明海洋附着细菌的附着动态及其在生物膜形成过程中的作用奠定了基础.     

黄海南部小黄鱼洄游分布及种群结构特征

Monthly fishery survey data of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea from2003 and 2013 were employed to evaluate the variation in the resource distribution and biological characteristics(especially body length and sex ratio) in the population on a decadal scale.The results indicated that the small yellow croaker migrated from the Shawai fishing ground to the Dasha fishing ground in spring and was mainly distributed in the central and western parts of the Dasha fishing ground in April and May.Larimichthys polyactis in the Dasha fishing ground migrated eastward to offshore wintering grounds in autumn and reached the central Dasha fishing ground in October and November.The small yellow croaker entered the western waters of the Shawai fishing ground in winter.A large number of age 0+ fish occurred in the Shawai and Jiangwai fishing grounds in October of 2003 and 2013.The body lengths of the spawning stock and wintering stock in 2013 were larger than those in 2003,and the monthly sex ratios(female to male) were significantly less than 1 in both years.The monthly distribution of this fish in the southern Yellow Sea was consistent with a previous finding that "the stock migrated between the wintering grounds in the west of Jeju Island and the Lüsi spawning grounds" but tended to move more northward,with the spawning grounds extending outward.In the past decade,body length variation experienced a decline after an increase,rather than a steady decrease.The sex ratio in the single-stick stow net showed a tendency to increase over the decade,but was either less than or more than 1 depending on the fishing gear;therefore,further studies should be conducted to determine the sex structure.