松江鲈(Trachidermus fasciatus)泛素结合酶E2-D2基因的分子克隆及组织表达分析

泛素结合酶(UbcE2)是蛋白泛素化过程中所必需的酶,在泛素转移和底物的特异性识别方面发挥着重要的作用。本实验利用RACE技术克隆获得了全长为993bp的松江鲈泛素结合酶E2-D2基因cDNA序列(命名为TfUbcE2-D2),其开放阅读框为444bp,编码147个氨基酸。通过SMART预测得知,TfUbcE2-D2含有一个UBCc结构域。同源比对结果表明该基因与其他物种的同源性为92.31%。实时荧光定量PCR显示,TfUbcE2-D2广泛表达于松江鲈各组织,在肾脏中的相对表达量最高,其次为鳃。鳗弧菌(Vibrio anguillarum)刺激后,TfUbcE2-D2在血液、脾脏、肝脏和鳃中表达均上调,其中,脾脏和鳃中的表达量上调约40倍。由以上实验结果推测,TfUbcE2-D2可能参与松江鲈的先天免疫防御。     

Simulation of the extreme waves generated by typhoon Bolaven (1215) in the East China Sea and Yellow Sea

Record-breaking high waves occurred during the passage of the typhoon Bolaven (1215) (TYB) in the East China Sea (ECS) and Yellow Sea (YS) although its intensity did not reach the level of a super typhoon. Winds and directional wave measurements were made using a range of in-situ instruments mounted on an ocean tower and buoys. In order to understand how such high waves with long duration occurred, analyses have been made through measurement and numerical simulations. TYB winds were generated using the TC96 typhoon wind model with the best track data calibrated with the measurements. And then the wind fields were blended with the reanalyzed synoptic-scale wind fields for a wave model. Wave fields were simulated using WAM4.5 with adjustment of C_d for gust of winds and bottom friction for the study area. Thus the accuracy of simulations is considerably enhanced, and the computed results are also in better agreement with measured data than before. It is found that the extremely high waves evolved as a result of the superposition of distant large swells and high wind seas generated by strong winds from the front/right quadrant of the typhoon track. As the typhoon moved at a speed a little slower than the dominant wave group velocity in a consistent direction for two days, the wave growth was significantly enhanced by strong wind input in an extended fetch and non-linear interaction.     

Prokaryotic community composition revealed by denaturing gradient gel electrophoresis in the East Sea

To understand the temporal and spatial variation of the prokaryotic community in the East Sea, their composition was determined by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE)-sequencing techniques. The investigations were conducted twice annually in 2007 and 2009 in coastal and offshore stations. Prokaryotic abundance (PA), leucine incorporation rate, and other environmental parameters were also measured. By using the DGGE approach, we obtained 283 bacterial sequences and 160 archaeal sequences. The most frequently detected bacterial phylotypes during the investigations belonged to Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes. However, their relative compositions differed in time and space. Although Alphaproteobacteria and Bacteroidetes were the dominant groups in the surface water in May 2007 and in May and October 2007, Gammaproteobacteria was dominant in mesopelagic samples. However, Gammaproteobacteria was overwhelmingly dominant in most samples in August 2009. Although Deltaproteobacteria was rarely found as a dominant bacterial group, it occupied the highest fraction in a mesopelagic sample in October 2007. Epsilonproteobacteria also showed a similar trend, although its maximal dominance was found in a mesopelagic sample in August 2009. The archaeal community was dominated overwhelmingly by members of the Euryarchaeota in most of the investigations. However, Nitrosopumilales was dominant in aphotic samples in August 2009. Further, their spatiotemporal composition at the family level changed more dynamically in the East Sea. These temporal and spatial distributions of the prokaryotic community were influenced mainly by seawater temperature and depth in the East Sea.     

Nonradial eruption of a kinking filament observed from STEREO

On 5 April 2008, a filament at the periphery of an active region was observed by the Extreme Ultraviolet Imager telescope aboard the STEREO-A spacecraft, which showed up as a prominence eruption in the field-of-view from STEREO-B. The filament at STEREO-A 304 Å was first lengthened toward a region with weak overlying magnetic field so evolved as a large-scale one consisting of bright and dark threads twisting with each other, and then the portion below the weak field underwent an eruption. Meanwhile, the corresponding STEREO-B 304 Å prominence threads exhibited a kinking structure and tilting motion, with its center deflecting from the radial direction. By using three-dimension (3D) reconstruction technology, we obtain the 3D topology for the kinked prominence when its apex arrived at 1.4 radii, from which a clockwise rotation of about 90° is found in the course of the eruption. By comparing the 3D structure with the magnetic-field configuration computed by using the Potential-Field Source-Surface (PFSS) model, it is suggested that the filament erupted against the rather weaker than stronger overlying magnetic field, which make it appear to tilt toward one side.     

Distribution of dinoflagellate cysts in Yellow Sea sediments

To investigate the distribution,abundance,and species composition of dinoflagellate cysts in the Yellow Sea,surface sediment samples were collected at 37 sites,including the Korean dump site.Twenty-one dinoflagellate cyst taxa were identified,with the assemblages dominated mainly by Spiniferites bulloideus,Operculodinium centrocarpum,and cyst of Alexandrium catenella/tamarense type.A high frequency of O.centrocarpum in the Yellow Sea was observed for the first time,and it is likely that this can be attributed to the dynamics of the Yellow Sea Cold Water Mass and the Changjiang(Yangtze) River runoff.Total cyst concentrations ranged from 23 to 48 442 cysts/g dry weight,and high cyst concentrations were recorded adjacent to the dumping site.This result suggests that anthropogenic activities such as ocean dumping stimulate the growth of dinoflagellates in the Yellow Sea,which in turn leads to high levels of dinoflagellate cyst production.     

攀钢新基地欠固结土层旋挖桩负摩阻力试验

攀钢新基地工程区80%为"昔格达"土回填区,回填土将对桩产生负摩阻力。现场负摩阻力试验表明:回填土上部在堆载情况下会有较大的固结沉降,从而使桩基产生负摩阻力。     

基于遥感数据的青藏高原热环境调查

本文以地球系统科学和地球力学为理论基础, 以遥感(RS)、地理信息系统(GIS)、全球定位系统(GPS)为技术支撑, 利用亚东－格尔木－锡铁山走廊域的地学剖面实测温度数据和青藏高原MODIS遥感影像数据, 分析研究了青藏高原亚东－格尔木－锡铁山走廊域的热环境特征。通过研究表明, 研究区域内热环境总体分布规律为中东部最高, 东南部次之, 西北部最低。