An application of Iatroscan thin-layer chromatography with flame ionization detection—lipid classes of microorganisms as biomarkers in the marine environment

Iatroscan TLC-FID combines the separatory capabilities of thin-layer chromatography (TLC) (on silica coated chromarods) with the possibility of quantitation by flame ionization detection (FID). It can be used to separate lipid extracts into classes of compounds ranging from neutral to polar products. The rapidity with which this system provides lipid class profiles from small samples makes it particularly useful in oceanographic studies where routine analyses of large numbers of samples are required. Lipid classes were analysed in 4 marine bacterial strains grown on various substrates and 10 microalgae isolated from the marine environment and grown in batch cultures. Data on lipid composition of these microorganisms were obtained and compared to literature data. Results show that ratios between classes of compounds within the different groups of microorganisms were characteristic enough to constitute a good print of bacterial or phytoplankton sources in organic matter in sea water. In a preliminary study, phospholipid classes from a bacterial strain and a microalga were separated. Results show that the determination of ratios between phospholipid classes in organic matter could represent an improvement in the utilization of lipid classes as biomarkers.     

Correlations among the reflectances of vitrinite, chitinozoans, graptolites and scolecodonts

Paleozoic marine sequences sampled in outcrops and wells of northeastern Gaspe Peninsula (Quebec Appalachians) yield kerogen composed in part of vitrinite and zooclasts (chitinozoans, scolecodonts and graptolites) on which reflectance measurements were made.The comparison between means of random measurements show that reflectance of chitinozoans is similar to that of telinite (vitrinite with a cellular texture) and that the reflectance of scolecodonts is significantly lower than that of telinite in the lower range of studied values. At higher reflectivities differences decrease but, at the end of the condensate gas zone reflectancea are not yet identical. As shown by previous studies, reflectance of graptolites is slightly less than that of telinite.Pyrobitumen reflectance is related to the lithology of the host-rock and no generalized correlation can be established between the reflectance of pyrobitumen and that of vitrinite.     

Assessment of wind-forcing impact on a global wind-wave model using the TOPEX altimeter

The study presents assessment of an operational wave model (Wavewatch III), focusing upon the model sensitivity to wind-forcing products. Four wind fields are used to drive the model, including the NCEP/NCAR reanalysis and three other products that assimilate various satellite wind measurements having high spatial resolution, including the QuikSCAT scatterometer. Three wave field statistics: significant wave height, mean zero-crossing wave period, and mean square slope are compared with collocated TOPEX altimeter derivatives to gauge the relative skill of differing wind-forced model runs, as well as to demonstrate an extended use of the altimeter beyond simply supplying wave height for wave model validation and assimilation. Results suggest that model output is critically sensitive to choice of the wind field product. Higher spatial resolution in the wind fields does lead to improved agreement for the higher-order wave statistics.     

Origin of components in Chilean thermal waters

Thermal waters of northern (18°–27°S) and southern (37°–45°S) Chile occur in two very different climatic, geologic and hydrologic environments: arid closed basins with abundant evaporites in the north; humid climate and well drained valleys in the south. The origin and behavior of the main components of the two groups of waters are examined and compared to each other. The modeling of the alteration of volcanic rocks leads to water compositions very different from those observed both in the north and south. In addition to hydrothermal alteration and deep emanations, the Cl/Br ratio reveals a major contribution of saline waters to the two groups: infiltrating brines from salt lakes in the north; seawater in the south.In the north, concentrations of Cl, Br, Na, K, Ca, SO₄, Li, B, Si result from the mixing of alteration waters with recycled brines. Hydrothermal alteration is obscured by this massive saline input, except for Mg. δ³⁴S values are consistent with an origin of sulfate from salar brines, which are themselves derived from deep Tertiary gypsum. In the south, two processes account for the composition of thermal waters: mixing of alteration waters with seawater and deep magmatic contribution. The mixing process controls the concentration of Cl, Br, Na, Alk, Si, K, Ca, Mg. Magmatic inputs are detectable for SO₄, Li and B. δ³⁴S suggests that sulfate stems from the mixing of alteration waters with either marine SO₄ in coastal waters or with deep SO₂ in inland waters. In both the north and south, the Mg concentration is drastically lowered (<1 μmol/L) by the probable formation of a chlorite-type mineral. In the south, very small amounts of seawater (<1% in volume) are sufficient to imprint a clear signature on thermal waters. Not only coastal springs are affected by seawater mixing, but also remote inland springs, as far as 150 km from the sea. Subduction of marine sediments in the accretive margin could be the source of the marine imprint in thermal waters of southern Chile. Seawater may be expelled from the subducted lithosphere and incorporated into the mantle source.     

Thermo-hydro-mechanical simulation of ATLAS in situ large scale test in Boom Clay

Following the need for understanding and quantifying the effect of temperature on the response of a candidate host formation for radioactive waste disposal, finite element modelling of an in situ thermal experiment has been carried out. Based on a thermo-hydro-mechanical (THM) finite element approach including a consistent thermo-plastic constitutive model, it has been possible to reproduce the THM response of a clay formation submitted to in situ thermal loading. The simulated large-scale experiment, called ATLAS was designed in the underground research facility (HADES-URF) in Mol, Belgium. After an extensive literature analysis on the thermal, hydraulic and mechanical characteristics of Boom Clay, laboratory tests were simulated to calibrate model parameters. The results of the finite element modelling of the ATLAS experiment were compared with in situ measurements and revealed the necessity to account for flow diffusion in all three directions through a 2D axisymmetric analysis. Finally, those results were interpreted in the light of elasto-thermoplasticity, which emphasizes the significant role of thermo-plastic processes in the global THM response of the clay formation.     

State and church in France: regulation and negotiation

In France the distinction between the secular and religious domains is the result of a slow evolutionary process of secularization over a period of two centuries. A key element was the law of separation between churches and State in 1905. Secularization also produced a pluralistic socio-cultural system, which permits an increase in religious supply, issued from outside or inside existent churches. The religious landscape became complex due to the growth of certain groups. Nowadays, efforts to regulate Muslim communities are leading towards a new tendency of state control of religious activities in two fields, namely places of worship and school activities. Religious plurality is recognized by the State in the name of the freedom of beliefs. Private organizations are used for the public practice of religion and easily obtain places of worship. However, this has necessitated a large number of regional or local arrangements. In the name of the freedom of thought, the school system of the Republic is ensured by public schools, or by Catholic or Jewish schools, subject to strict public control. In this matter, there is only minimal tolerance of other denominations.