Sample preparation methods for quantitative detection of DNA by molecular assays and marine biosensors

The need for quantitative molecular methods is growing in environmental, food, and medical fields but is hindered by low and variable DNA extraction and by co-extraction of PCR inhibitors. DNA extracts from Enterococcus faecium, seawater, and seawater spiked with E. faecium and Vibrio parahaemolyticus were tested by qPCR for target recovery and inhibition. Conventional and novel methods were tested, including Synchronous Coefficient of Drag Alteration (SCODA) and lysis and purification systems used on an automated genetic sensor (the Environmental Sample Processor, ESP). Variable qPCR target recovery and inhibition were measured, significantly affecting target quantification. An aggressive lysis method that utilized chemical, enzymatic, and mechanical disruption enhanced target recovery compared to commercial kit protocols. SCODA purification did not show marked improvement over commercial spin columns. Overall, data suggested a general need to improve sample preparation and to accurately assess and account for DNA recovery and inhibition in qPCR applications.     

Interim targets and the climate treaty regime

Abstract

We propose that international climate change policy would be strengthened by the development and adoption of targets for atmospheric concentrations of greenhouse gases 25–50 years in the future in addition to near- and long-term targets. ‘Interim concentration targets’, which could be accommodated under the current Convention/Protocol framework, would provide several advantages over the current focus on either the short term (e.g. Kyoto Protocol) or the long term (e.g. ultimate stabilization targets). Interim targets would help constrain rates of climate change (which are not sufficiently addressed by short- or long-term targets, even when paired together). They would also provide a means for keeping open the option of achieving a range of long-term goals while uncertainty (and political disagreement) over the appropriate goal is resolved. We substantiate a number of rationales for such an approach, discuss the use of interim targets in other contexts, and illustrate how such targets for climate change policy might be set.     

Assessing temporary carbon sequestration and storage projects through land use, land-use change and forestry: comparison of dynamic life cycle assessment with ton-year approaches

In order to properly assess the climate impact of temporary carbon sequestration and storage projects through land-use, land-use change and forestry (LULUCF), it is important to consider their temporal aspect. Dynamic life cycle assessment (dynamic LCA) was developed to account for time while assessing the potential impact of life cycle greenhouse gases (GHG) emissions. In this paper, the dynamic LCA approach is applied to a temporary carbon sequestration project through afforestation, and the results are compared with those of the two principal ton-year approaches: the Moura-Costa and the Lashof methods. The dynamic LCA covers different scenarios, which are distinguished by the assumptions regarding what happens at the end of the sequestration period. In order to ascertain the degree of compensation of an emission through a LULUCF project, the ratio of the cumulative impact of the project to the cumulative impact of a baseline GHG emission is calculated over time. This ratio tends to 1 when assuming that, after the end of the sequestration project period, the forest is maintained indefinitely. Conversely, the ratio tends to much lower values in scenarios where part of the carbon is released back to the atmosphere due to e.g. fire or forest exploitation. The comparison of dynamic LCA with the ton-year approaches shows that it is a more flexible approach as it allows the consideration of every life cycle stage of the project and it gives decision makers the opportunity to test the sensitivity of the results to the choice of different time horizons.     

Analysis of the seismicity in the central part of the Pyrenees (France), and tectonic implications

Two temporary seismological networks have been set up in 2000 and 2002 in the central part of the Pyrenees, in a region, which appears as a transition between two domains where both the seismic activity and the tectonic regime are different. Together with the permanent networks, they allowed us to obtain precise hypocenter locations for more than 400 events with local magnitudes ranging from 1.5 to 4.6, as well as 30 new focal mechanisms. The seismicity is distributed in several clusters, which are not located along the North Pyrenean Fault, considered as the major tectonic accident resulting from the suture of the Iberian and Eurasian convergent plates when the range formed. Several small fault segments dipping to the north are identified. The maximum focal depth varies from 10 to 20 km, with variations which are roughly parallel to those of the Moho, indicating a thickening of the seismogenic layer to the east of the studied area. The obtained focal solutions reveal a predominance of normal faulting to the West and reverse faulting to the East, with strike-slip motions in between. The largest fault segment to the East, with a length of about 20 km, could possibly be related to a large historical event which occurred in 1660, with intensity IX, close to cities which have become since then important touristic centres.     

Random walk of the Earth's pole related to the Chandler wobble excitation

Summary. The equation governing the polar motion shows that the polar secular drift and the Chandler wobble amplitude are related to each other. In particular, a drift of the mean pole position comes out as a consequence of the maintenance of the Chandler wobble by possible step perturbations of the Earth's inertia tensor.
The minimum excitation functions necessary to explain the Chandler wobble amplitude variations for the period 1901–84 are derived from the Chandler term, with the hypothesis that the excitations follow a uniform random distribution in time. It is shown that they have the statistical properties of the steps of a two-dimensional random walk. These functions are then used to derive, from a statistical simulation, a lower limit of the secular drift which may result from the excitation of the Chandler wobble.
The drift generated by the random walk is of the same order of magnitude as the observed secular drift for the period 1901–84, but their time dependence is different. This indicates that the observed secular drift cannot be explained as the consequence of an excitation of the Chandler wobble by random steps of the Earth's inertia tensor. However, the possible contribution of the Chandler wobble excitation to the polar drift has to be taken into account when other mechanisms, such as lithospheric rebound related to deglaciation, are proposed.     

Tree ring analysis of hydro‐climatic thresholds that trigger ice jams on the Mistassini River,Quebec

This study documents the spatiotemporal variations in the frequency and magnitude of ice jams in the Mistassini River and applies that information to the identification of the hydro‐climatic threshold conditions associated with major events. Ice jams cause severe upheavals in water flow, which result in flooding upstream of the ice jam front, and therefore represent a significant geohazard to riparian populations. To analyze the spatiotemporal variations in the magnitude–frequency of ice jams, the Mistassini River was first divided into six different sites representing different geomorphological contexts. A 50‐year ice jam chronology was constructed from 85 damaged trees from all of the study sites. This chronology was then coupled with hydro‐climatic variables to construct classification trees, which helped identify the conditions and hydro‐climatic thresholds favourable to the triggering of ice jams in a predictive model. The results indicate complex interactions between the characteristics of flow, ice cover and river morphology that affect the frequency and magnitude of ice jam events on the Mistassini River. These factors affect the frequency and magnitude of ice jam events. The triggering of extreme events seems particularly influenced by exceptional ice conditions and sites with high sinuosity and islands. Copyright © 2015 John Wiley & Sons, Ltd.