Estimation of Hydraulic Conductivity and Its Uncertainty from Grain-Size Data Using GLUE and Artificial Neural Networks

Various approaches exist to relate saturated hydraulic conductivity (K _s) to grain-size data. Most methods use a single grain-size parameter and hence omit the information encompassed by the entire grain-size distribution. This study compares two data-driven modelling methods??multiple linear regression and artificial neural networks??that use the entire grain-size distribution data as input for K _s prediction. Besides the predictive capacity of the methods, the uncertainty associated with the model predictions is also evaluated, since such information is important for stochastic groundwater flow and contaminant transport modelling. Artificial neural networks (ANNs) are combined with a generalised likelihood uncertainty estimation (GLUE) approach to predict K _s from grain-size data. The resulting GLUE-ANN hydraulic conductivity predictions and associated uncertainty estimates are compared with those obtained from the multiple linear regression models by a leave-one-out cross-validation. The GLUE-ANN ensemble prediction proved to be slightly better than multiple linear regression. The prediction uncertainty, however, was reduced by half an order of magnitude on average, and decreased at most by an order of magnitude. This demonstrates that the proposed method outperforms classical data-driven modelling techniques. Moreover, a comparison with methods from the literature demonstrates the importance of site-specific calibration. The data set used for this purpose originates mainly from unconsolidated sandy sediments of the Neogene aquifer, northern Belgium. The proposed predictive models are developed for 173 grain-size K _s-pairs. Finally, an application with the optimised models is presented for a borehole lacking K _s data.     

Field evidence of hydraulic connections between bedrock aquifers and overlying granular aquifers: examples from the Grenville Province of the Canadian Shield

Field evidence of hydraulic connections between a bedrock aquifer and an overlying granular aquifer in the Canadian Shield (Grenville Province) is presented. This issue is rarely considered and investigated despite its important hydraulic and chemical consequences and its widespread occurrence worldwide. The methodology employed is based on complementary field tests conducted at specific experimental sites instrumented both in the rock and in the overlying deposits. One of the bedrock sites revealed a natural hydraulic connection with the overlying granular aquifer caused by the weathered surface of the uppermost bedrock. Another site revealed an artificial hydraulic connection between the bedrock and the granular aquifer created by an improperly sealed casing. A regional study showed that hydraulic connections yield an erroneous interpretation of the true hydraulic properties of the tested aquifer. The detection of hydraulic connections is therefore essential to properly define well-capture areas and contamination conditions. It is recommended to practitioners that pumping tests be performed as well as hydrochemical comparisons of each existing aquifer unit. Falling-head permeability tests are also helpful in verifying the quality of the seal at the bedrock–casing contact. More effective procedural controls and better well-construction practices are necessary to reduce the risks of cross-contamination induced by defective seals.     

A two level scaling-up method for multiphase flow in porous media; numerical validation and comparison with other methods

We present a robust and accurate strategy for upscaling two-phase flow in heterogeneous porous media composed of different rock-types. The method is tested by means of numerical simulations and compared with other upscaling methods.     

Long-term Field Observations on Seasonality in Chlorophyll-<Emphasis Type="Italic">a</Emphasis> Concentrations in a Shallow Coastal Marine Ecosystem,the Wadden Sea

Analyses of long-term field observations (1974–2007) on chlorophyll-a concentrations in the western Wadden Sea showed no long-term trends in the timing of the wax and wane of phytoplankton spring blooms. There is weak evidence, however, that the height of the autumn bloom has decreased since the early 1990s. This fading of the autumn bloom may have had consequences for the carbon transfer to higher trophic levels, currently hampering primary consumer species that mostly rely on food supply during late summer. Current and other findings suggest a shortening of the growing season due to the fading of the autumn bloom in the Wadden Sea and a lengthening of the growing season due to an advancement of the spring bloom in the North Sea. These regionally different changes in seasonality may have contributed to the coinciding decrease in bivalve filtering capacity in the western Wadden Sea and the large-scale offshore shift of juvenile plaice from the Wadden Sea to the adjacent North Sea.     

Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture.The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(L.vannamei) were investigated.This involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and gills.Three experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of 25.After 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels.Subsequently,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and 2.Shrimp fed with 40% protein at 25 in salinity(optimal conditions) were used as a control.Regardless of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the control.Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks.Ambient salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 weeks.These findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism.Increased dietary protein level could improve the osmoregulation capacity of L.vannamei with more energy resources allocated to GDH activity and expression.     

Characterization of Mediterranean magnetotactic bacteria

Magnetotactic bacteria are a diverse group of motile prokaryotes that are ubiquitous in aquatic habitats and cosmopolitan in distribution. In this study, we collected magnetotactic bacteria from the Mediterranean Sea. A remarkable diversity of morphotypes was observed, including multicellular types that seemed to differ from those previously found in North and South America. Another interesting organism was one with magnetosomes arranged in a six-stranded bundle which occupied one third of the cell width. The magnetosome bundle was evident even under optic microscopy. These cells were connected together and swam as a linear entire unit. Magnetosomes did not always align up to form a straight linear chain. A chain composed of rectangle magnetosomes bent at a posi- tion with an oval crystal. High resolution transmission electron microscopy analysis of the crystal at the pivotal position suggested uncompleted formation of the crystal. This is the first report of Mediterranean magnetotactic bacteria, which should be useful for studies of biogeochemical cycling and geohistory of the Mediterranean Sea.