应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)

参照鱼类病毒性出血性败血症病毒序列,根据PCR引物设计的原则,设计巢式PCR引物,采用巢式逆转录聚合酶链反应(RT-PCR)方法,对如何快速检测鱼类病毒性出血性败血症病的方法进行了较为系统的研究,并对RT-PCR的反应条件进行了优化.结果表明,巢式RT-PCR扩增获得279bp的特异性片断,阴性对照无扩增条带.巢式RT-PCR扩增出的特异性片段经测序分析,结果证实与报道的序列完全一致.该方法最低可检测出0.1pg的鱼类病毒性出血性败血症病毒RNA.初步建立了VHSV的巢式RT-PCR检测方法,该方法灵敏、特异,可为VHSV的检测提供一个快速、有效的手段.     

Preprocessing photospheric vector magnetograms for nonlinear force-free field extrapolation of the global corona

Nonlinear force-free magnetic field(NLFFF) extrapolation based on the observed photospheric magnetic field is the most important method to obtain the coronal magnetic field nowadays.However, raw photospheric magnetograms contain magnetic forces and small-scale noises, and fail to be consistent with the force-free assumption of NLFFF models. The procedure for removing the forces and noises in observed data is called preprocessing. In this paper, we extend the preprocessing code of Jiang Feng to spherical coordinates for a full sphere. We first smooth the observed data with Gaussian smoothing, and then split the smoothed magnetic field into a potential field and a non-potential field.The potential part is computed by a numerical potential field model, and the non-potential part is preprocessed using an optimization method to minimize the magnetic forces and magnetic torques. Applying the code to synoptic charts of the vector magnetic field from SDO/HMI, we find it can effectively reduce the noises and forces, and improve the quality of data for a better input which will be used for NLFFF extrapolations applied to the global corona.     

青海唐古拉地区侏罗纪非海相双壳动物群及其古环境分析

唐古拉山温泉以东约80km的冬曲一带产出丰富的侏罗纪非海相双壳类化石,中侏罗统雀莫错组顶部的紫红色细粒岩屑砂岩产Qiyangia,Yunnanopharus,Unio 3属3种,其中有新种Yunnanopharus substratus.这一动物群与过去在本区发现的中侏罗统“Cuneopsis”-Eolamprotula-Protomya动物群面貌明显不同,过去一直认为Yunnanopharus是晚三叠世的属,而Qiyangia则主要产于下侏罗统,根据地层层序及上、下层位产出的海相双壳类和腕足类化石时代分析,冬曲剖面的Qiyangia-Yunnanopharus动物群时代无疑为中侏罗世巴通期,扎窝茸组产非海相双壳类“Cuneopsis”,Yunnanopharus,Plicatunio,Nipponaia(Eonipponaia),Cyotrigonioides,Danlengicon-cha,Nakamuranaia等7属9种,其中包括新种Yunnanoconcha(?)ovalis,Danlengiconha tanggu-laensis,Cyotrigonioides(?)ellipticus和Nipponaia(Eonipponaia)?bifurcata,基于下伏海相地层中双壳类和腕足类化石的佐证,它们的时代最有可能是在晚侏罗世基末里期至早白垩世期间,据化石围岩的粒度分析以及埋藏学研究表明,这些类三角蚌类主要生活于水体安静、有机质含量丰富的淤泥质基底,繁盛于近海三角洲平原上的湖沼环境,同时,由于海退初期尚有可能受到海水短暂侵漫的影响,造成它们生存环境的咸化     

潜水的降雨入渗补给模型研究

根据土水势能原理,在试验、监测资料基础上,建立活塞武、扩散式两种入渗补给模型和单一入渗、单一蒸发及入渗蒸发三个子模型,与此相应地建立了积水模型法,表面通量法,毛细带上缘通量法和零通量面法的数学模型,描述入渗补给的时、空变化过程,计算降雨入渗补给量,从土水势能观点提出降雨入渗补给的物理机制,并在实际应用中得到了证实。     

低氟与大骨节病－－大骨节病防治拾趣四则

该文笔者多年研究大骨节病原始原因的一项新说。即病区外环境低氟。引起体内以低氟为中心的代谢紊乱，从而导致以骨骼系统为主的病变－大骨节病。防治该病，应向体外补氟。药疗这一进补方式故然必然，但最有效又生活化的办法是食疗。食疗又分氟盐防治和饮茶防治两种。而最适用且简便的方法是提倡患区人群适度饮茶，并且应从儿时做起。关于补氟和饮茶等防治大骨节病的实践，笔者已浓缩于下面四则拾趣之中……。     

华南燕山晚期构造-岩浆事件与成矿作用——来自广西大瑶山龙头山金矿床的地球化学约束

广西贵港大瑶山成矿带位于华南东南沿海成矿带西南端,对矿带内龙头山金矿黄铁矿化斑岩进行定年的结果为96.1±3.0 Ma,与区域内邻近的平天山侵入岩体(96.2±0.4 Ma)相同.龙头山矿床的Sr同位素组成与其锆石的REE特征表明,成矿作用伴随有大量的流体参与;矿化斑岩与区内侵入相岩石相同的形成时代以及矿化斑岩中锆石C...     

福建尤溪梅仙矿床闪锌矿中黄铜矿交生体的交代成因

福建尤溪梅仙块状硫化物矿床中的闪锌矿经５０％ＨＮＯ３浸蚀后发现,微米级的黄铜矿交生体呈乳滴状,棒条状,蠕虫状等沿闪锌矿解理面,双晶面和颗粒边界,裂隙等处分布,并且黄铜矿交生体所占体积均在５％以上,因上闪锌矿中有限的Ｃｕ的溶解度使出溶无法解释黄鲷矿交生体的成因,交生体的结构特征以其与黄铜矿脉的关系暗示其可能是交代成因。而对横切黄铜矿乳滴的电子探针分析中铁含量特征的研究,证实梅仙矿区闪锌矿中的黄铜矿交     

Distributions of n-alkanes and their hydrogen isotopic composition in plants from Lake Qinghai (China) and the surrounding area

Various aquatic plants from Lake Qinghai, the largest inland saline lake in China, and terrestrial plants from the surrounding area were investigated for the distribution of n-alkanes and their δD values. The n-alkanes in the samples range from C₁₅ to C₃₃ with C preference index (CPI) values of 4.0–29.7. The n-C₂₃ or n-C₂₅ alkane is the dominant compound in the aquatic submerged plants. The aquatic emergent and terrestrial plants have an abundance maximum at n-C₂₇, n-C₂₉ or n-C₃₁. The average chain length (ACL) values, ranging from 26.0 to 29.6, are closely related to the plant species. The n-alkanes from the aquatic plants have mean δD values of −169‰ to −121‰ and those from the terrestrial plants values of −173‰ to −109‰. The H isotopic composition (δD) and fractionation differ significantly among the plants studied. Comparison shows that additional evaporative enrichment of the lake water associated with saline lakes and humidity influence the δD values of the n-alkanes in aquatic and terrestrial plants, respectively. The mean δD values of n-alkanes in the plants decrease with increasing ACL value. The n-alkanes from the different types of plants are more depleted in D relative to environmental water and those from aquatic plants (with a mean value of −143‰) have a greater isotopic fractionation than terrestrial plants (mean value −113‰).     

Geological, geochemical characteristics and isotope systematics of the Longqiao iron deposit in the Lu-Zong volcano-sedimentary basin, Middle-Lower Yangtze (Changjiang) River Valley, Eastern China

The Middle-Lower Yangtze (Changjiang) River Valley metallogenic belt is located on the northern margin of the Yangtze Craton of eastern China. Most polymetallic deposits in the Changjiang metallogenic belt are clustered in seven districts where magmatism of Mesozoic age (Yanshanian tectono-thermal event) is particularly extensive. From west to east these districts are: E-dong, Jiu-Rui, Anqing-Guichi, Lu-Zong, Tong-Ling, Ning-Wu and Ning-Zhen. World-class iron ore deposits occur in the Lu-Zong and Ning-Wu ore clusters, which are mainly located in continental fault-bound volcanic-sedimentary basins. One of these deposits is the Longqiao iron deposit, discovered in the northern part of the Lu-Zong Basin in 1985. This deposit consists of a single stratabound and stratiform orebody, hosted in sedimentary carbonate rocks of the Triassic Dongma'anshan Formation. A syenite pluton (Longqiao intrusion) is situated below the deposit. The iron ore is massive and disseminated and the ore minerals are mainly magnetite and minor pyrite. Wall rock alteration mostly consists of skarn minerals, such as diopside, garnet, potassic feldspar, quartz, chlorite, phlogopite and anhydrite. Thin sedimentary siderite beds of Triassic age occur as relict laminated ore at the top and the margin of the magnetite orebody. These sideritic laminae are part of Triassic evaporite-bearing carbonate deposits (Dongma'anshan Formation).Sulfur isotopic compositions show that the sulfur in the deposit was derived from a mixture of magmatic hydrothermal fluids and carbonate–evaporite host rocks. Similarly, the C and O isotopic compositions of limestones from the Dongma'anshan Formation indicate that these rocks interacted with magmatic hydrothermal fluids. The O isotopic compositions of the syenitic rocks and minerals from the deposit show that the hydrothermal magnetite and skarn minerals were formed from magmatic fluids. The Pb isotopic compositions of sulfides are similar to those of the Longqiao syenite. Phlogopite coexisting with magnetite in the magnetite ores yielded a plateau age of 130.5 ± 1.1 Ma (2σ), whereas the LA-ICP MS age of the syenite intrusion is 131.1 ± 1.5 Ma, which is slightly older than the age of phlogopite.The Longqiao syenite intrusion may have crystallized from a parental alkaline magma, generated by partial melting of lithospheric mantle, during extensional tectonics. The ore fluids were probably first derived from magma at depth, later emplaced in the sedimentary rocks of the Dongma'anshan Formation, where it interacted with siderite and evaporite-bearing carbonate strata, resulting in the formation of magnetite and skarn minerals. The Longqiao iron deposit is a skarn-type stratabound and stratiform mineral system, genetically and temporally related to the Longqiao syenite intrusion. The Longqiao syenite is part of the widespread Mesozoic intracontinental magmatism (Yanshanian event) in eastern China, which has been linked to lithospheric delamination and asthenospheric upwelling.     

通过第一性原理预测内地核的成分与结构

内地核成分与结构的确定一直是地球深部研究的重要课题。目前地核的公认成分是铁和少量的镍。但由于地核密度低于纯粹的铁镍合金(固态内核2%～3%,液态外核6%～7%),其中必定掺杂有一定量的轻元素,其种类与浓度有待确定。除成分外,地核条件下铁的晶体结构也存在争议。根据地震学观测,声波沿地轴方向的传播速度比赤道平面方向快大约3%～4%。这意味着内地核是各向异性的;但在极端高压下,晶体结构中的原子应该按致密的密排六方结构(h.c.p)排列,而h.c.p结构对声波传输是高度各向同性的,这就需要确定地核条件下铁的晶体结构。根据第一性原理计算得到的高压下体系能量以及爱因斯坦谐振子模型,本项研究估算了给定结构的自由能以及掺杂轻元素后的影响。根据计算结果可以定性的分析得出,在高压OK下致密的h.c.p结构显然比疏松的体心立方(b.c.c)更稳定;而随着温度的升高,原子核的振动造成b.c.c结构的自由能比h.c.p结构下降得更快,因此在高温下b.c.c结构更稳定;掺杂轻元素后,这种优势变得更加明显,而3.6at.%的Si则恰好同时解释了2%～3%的密度缺失和b.c.c结构在内地核条件下的稳定性。因此我们建议内地核的基本结构与成分应为以体心立方结构存在的铁,掺杂约3.6at.%的硅元素,内地核温度至少在5500K以上。这一结论与其它更复杂的方法得到的结果一致。