Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR

Marine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health.     

Enhancement of biological productivity by internal waves: observations in the summertime in the northern South China Sea

Streaks of elevated concentrations of surface chlorophyll a (Chl_a) of various spacing were found to be associated with internal waves in their transmission zone and dissipation zone in the summertime in the deep open northern South China Sea. At an anchored station in the dissipation zone north of the Dongsha Atoll with a water depth of ca. 600?m, undulations of the mixed layer depth with an amplitude of ca. 30?m and a periodicity of ca. 12?h were observed, and they were accompanied by similar undulation in the isotherm and isopleth of the nutrients. These observations are consistent with the enhancement of vertical mixing by internal waves and the resulting transfer of cold, nutrient-rich subsurface water to the surface mixed layer to fuel biological productivity. In the transmission zone and dissipation zone, respectively, the summertime (May–October) average sea surface temperature was 0.5 and 0.8?°C lower and Chl_a was 19 and 43?% higher than those in a nearby subregion that was minimally affected by internal waves. The mean net primary productivity was elevated by 15 and 37?%. These results indicate that the enhancement of biological activity by internal waves is not confined to the shallow waters on the shelf. The effect can be detected in all phases of the internal waves although it may be especially prominent in the dissipation zone where mixing between subsurface and surface waters is more effective.     

Health impact assessment of marine emissions in Pearl River Delta region

Global marine vessels emissions are adversely affecting human health particularly in southeast Asia. But health burdens from both ocean- and river-going vessels in Pearl River Delta (PRD) regions are not quantified. We estimated the potential health impacts using pooled relative risks of mortality and hospital admissions in China, and the model derived concentrations of sulfur dioxide (SO₂), particulate matter (PM₁₀), nitrogen dioxide (NO₂) and ozone (O₃) due to vessels emissions. SO₂ concentrations due to marine emissions in Hong Kong were 13.6 μgm^?3 compared with 0.7 μgm^?3 in PRD regions that were far from the marine vessels. In PRD regions, the estimated annual numbers (per million people) of excess deaths from all natural causes and hospital admissions from cardiorespiratory causes attributable to SO₂, NO₂, O₃ and PM₁₀ combined from marine emissions were 45 and 265 respectively. Marine emission control measures could contribute a large reduction in mortality and hospital admissions in PRD regions especially in Hong Kong.     

Cracking Processes in Rock-Like Material Containing a Single Flaw Under Uniaxial Compression: A Numerical Study Based on Parallel Bonded-Particle Model Approach

Cracking processes have been extensively studied in brittle rock and rock-like materials. Due to the experimental limitations and the complexity of rock texture, details of the cracking processes could not always be observed and assessed comprehensively. To contribute to this field of research, a numerical approach based on the particle element model was used in present study. It would give us insights into what is happening to crack initiation, propagation and coalescence. Parallel bond model, a type of bonded-particle model, was used to numerically simulate the cracking process in rock-like material containing a single flaw under uniaxial vertical compression. The single flaw’s inclinations varied from 0° to 75° measured from the horizontal. As the uniaxial compression load was increased, multiple new microcracks initiated in the rock, which later propagated and eventually coalesced into longer macrocracks. The inclination of the pre-existing flaw was found to have a strong influence on the crack initiation and propagation patterns. The simulations replicated most of the phenomena observed in the physical experiments, such as the type, the initiation location and the initiate angle of the first cracks, as well as the development of hair-line cracks, which later evolved to macrocracks. Analyses of the parallel bond forces and displacement fields revealed some important mechanisms of the cracking processes. The first cracks typically initiated from the tensile stress concentration regions, in which the tensile stress was partially released after their initiation. The tensile stress concentration regions subsequently shifted outwards close to the propagating tips of the first cracks. The initiation and propagation of the first cracks would not significantly influence the compressive stress singularity at the flaw tips, which was the driving force of the initiation of secondary cracks. The initiation of microcracking zone consisting almost exclusively of micro-tensile cracks, and that of microcracking zone consisting of micro-tensile cracks and mixed micro-tensile and shear cracks, were found to be correlated with two distinct types of displacement fields, namely type I (DF_I) and type II (DF_II), respectively.     

Assessment of risk of PCDD/Fs and dioxin-like PCBs in marine and freshwater fish in Pearl River Delta, China

Fish consumption is known to be beneficial to human health. However since the age of industrialization, the released/disposed chemical pollutants into water systems make fish a source of various environmental toxicants to humans. In oceanic cities with heavy industrial activities, fish products contribute the greatest proportion of exposure to pollutants. In this study, risks and potential effects of dioxins to health of coastal populations in the Pearl River Delta were assessed. Concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (PCBs) were measured in common fish species purchased at local markets. Concentrations of total dioxins in fish ranged from 0.481 to 9.05 pg TEQ/g wet weight were similar to the lesser concentrations reported for fish from other countries. The greatest concentrations of dioxins were measured in mandarin fish, a carnivorous freshwater fish. Exposure of murine primary leydig and ovarian cells to 2,3,7,8-tetrachlorinated dibenzo-p-dioxins (2,3,7,8-TCDD) reduced the synthesis of progesterone, testosterone and/or estrogen. The reductions were probably via inhibitory effects on the expression of the steroidogenic enzymes, steroidogenic acute regulatory protein (StAR) and cytochrome P450 side-chain cleavage enzyme (P450scc). Based on these reproductive parameters, the concentrations of dioxins and dioxin-like residues represent a moderate health risk due to consumption of fish.     

Laboratory Validation of Ultra-Soft Soil Deformation Model

Ultra-soft soil with high moisture content will experience large strain deformation under one-dimensional compression with little or no gain in effective stress. Such deformation behaviour does not comply with Terzaghi’s effective stress gain theory. The e-log s_v^￠ \sigma_{v}^{\prime } relationship of ultra-soft soil is non-linear with large compression index in the first order of log cycle. This paper proposes three compression indices (C_c1^* C_{c1}^{*} , C_c2^* C_{c2}^{*} and C_c3^* C_{c3}^{*} ) for stresses covering three log cycles. Good prediction of settlement magnitude is possible with these newly proposed compression parameters for ultra-soft soil. In addition, implicit finite difference model applying the large strain theory is also proposed and validated with results from laboratory measurements. The time factor curves for ultra-soft soil with large strain compression are also proposed and validated.     

U–Pb and Hf isotopic study of zircons from migmatised amphibolites in the Cathaysia Block: Implications for the early Paleozoic peak tectonothermal event in Southeastern China

Amphibolites occur in a number of localities in the Cathaysia Block, some of them have been migmatised and their protoliths represent basaltic magmas erupted in various tectonic settings. Four migmatised amphibolites were collected from Jiangxi and Fujian Provinces. Cathodo-luminescence images of zircons extracted from the representative amphibolites show unzoned or sector-zoned structure. LA-ICP-MS analysis indicates that most zircons have high Th/U ratios and yield U–Pb zircon ages of 446 ± 5, 435 ± 2, 434 ± 4 and 423 ± 2 Ma, respectively. Lu–Hf isotopic analysis on these zircons gives Hf model ages ranging from 900 to 1200 Ma. Based on lithological observations and previously published geochronological data, we interpret that these U–Pb ages record an important tectonothermal event that led to the migmatization. This early Paleozoic (Caledonian) tectonothermal event in the Southeastern China has a great tectonic implication for the evolutionary history of the Cathaysia Block.     

Dynamic Study on Fracture Problems in Viscoelastic Sedimentary Rocks Using the Numerical Manifold Method

The viscoelastic deformation behavior of a sedimentary rock under different loading rates is numerically modeled and investigated by the numerical manifold method (NMM). By incorporating a modified 3-element viscoelastic constitutive mode in the NMM, crack initiation and propagation criteria, and crack identification and evolution techniques, the effects of the loading rates on the cracking behavior of a sedimentary rock, such as crack open displacement, crack sliding displacement, crack initiation, crack propagation and final failure mode, are successfully modeled. The numerical results reveal that under a high loading rate (>1,000 MPa/s), due to the viscoelastic property of the sedimentary rock, not only the structural behavior deviates from that of elastic model, but also different cracking processes and final failure modes are obtained.