平顶山市矿业特点及发展对策浅议

本文从当前平顶山市矿业特点及引发的问题出发,着重指出实现矿业经济可持续发展,必须加大矿业结构的调整,并从资源管理方式、资源配置、矿业秩序、地勘工作等方面作了较为详细的论述。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

Hoek—Brown准则在主应力平面表示形式的讨论

Hoed-Brown准则是国际上有名的岩体经验强度准则，考虑了应力状态对岩体破坏特性的影响，在系统介绍H－B准则在主应力平面p,q表示形式的基础上，重点对其理论意义和实际应用中的关键问题进行分析讨论，以加深对该准则的理解。     

我国科学数据共享现状的调查

本文是科学数据共享政策法规体系研究系列文章的收笔之作.撰写本文的目的,就是为说明科学数据共享政策法规体系的研究是建立在大量的基础调研之上.调查研究是谋事之基、成事之道.从实践中寻求解决现实问题的方法,才能制定出切实可行的政策、法规,调查研究是制定政策、法规的客观依据.为使制订政策、 规章制度适应社会经济发展和变化的新情况,就深入开展调查研究,认真听取各方面的意见,深入研究相应的管理规律和运行机制.本文详述了调查过程、调查方法,以佐证政策法规研究不能脱离实际.     

Relationships between Interannual and Intraseasonal Variations of the Asian-Western Pacific Summer Monsoon Hindcasted by BCC_CSM1.1（m）

Using hindcasts of the Beijing Climate Center Climate System Model, the relationships between interannual variability （IAV） and intraseasonal variability （ISV） of the Asian-western Pacific summer monsoon are diagnosed. Predictions show reasonable skill with respect to some basic characteristics of the ISV and IAV of the western North Pacific summer monsoon （WNPSM） and the Indian summer monsoon （ISM）. However, the links between the seasonally averaged ISV （SAISV） and seasonal mean of ISM are overestimated by the model. This deficiency may be partially attributable to the overestimated frequency of long breaks and underestimated frequency of long active spells of ISV in normal ISM years, although the model is capable of capturing the impact of ISV on the seasonal mean by its shift in the probability of phases. Furthermore, the interannual relationships of seasonal mean, SAISV, and seasonally averaged long-wave variability （SALWV; i.e., the part with periods longer than the intraseasonal scale） of the WNPSM and ISM with SST and low-level circulation are examined. The observed seasonal mean, SAISV, and SALWV show similar correlation patterns with SST and atmospheric circulation, but with different details. However, the model presents these correlation distributions with unrealistically small differences among different scales, and it somewhat overestimates the teleconnection between monsoon and tropical central-eastern Pacific SST for the ISM, but underestimates it for the WNPSM, the latter of which is partially related to the too-rapid decrease in the impact of E1 Nifio-Southern Oscillation with forecast time in the model.     

我国短期气候动力预测模式系统的研究及试验

气候和气候异常对我国的国民经济发展具有重大影响,为提高短期气候预测的准确率,研究动力气候模式短期气候预测新技术至关重要.通过近5年的努力,建立了一套出月动力延伸预报模式,海气耦合的全球气候模式(AGCM+OGCM+海冰+高分辨率印度洋-太平洋海盆模式),区域气候模式季和年际尺度的业务动力模式组成的系统.初步把我国的短期气候预测水平由经验统计方法提高到定量和客观分析的水平上.在此基础上,已建成了一个具有物理基础的统计方法与气候动力模式相结合的综合气候预报系统.     

黄河中游一次致洪暴雨过程的形成机理

利用常规观测资料、NCEP再分析资料等,对黄河中游2013年7月21-22日的致洪暴雨过程的形成机理进行分析。结果发现:1)地面辐合线的形成和维持是本次过程降水强度大和降水集中的主要原因。中尺度雨团和地面中尺度辐合线相对应,地面中尺度辐合线的形成和冷空气扩散补充相对应。第一次冷空气扩散,促使长武站附近地面中尺度辐合线的形成和维持;第二次冷空气补充,促使北洛河流域、无定河流域的地面中尺度辐合线形成并维持。2)暴雨过程发生前,其上空存在干暖盖的结构特征,是能量积累及位势不稳定层结结构建立的关键;暴雨发生过程中干空气侵入对中尺度对流云团起激发作用。3)暴雨、大暴雨发生过程中水汽的垂直输送明显,暴雨区上空1000-100 h Pa相对湿度均在90%以上。     

青藏高原热力异常与华北汛期降水关系的研究

利用1980～1994年NCEP/NCAR再分析资料,以及我国336个测站1956～1994年月降水量资料,通过诊断分析和数值实验,研究了夏季高原上热力异常与华北汛期降水的关系.结果表明:华北汛期干旱年,青藏高压及西太平洋副热带高压偏南、偏东,华北汛期降水偏多年则相反;华北汛期旱年时,高原上升、高原东侧邻近地区下沉的垂直环流明显加强,而降水偏多年时,垂直环流减弱,华北地区为上升气流控制;夏季高原为热源和水汽汇区,它们的异常对华北地区降水有很大影响,当热源和水汽汇增强(减弱)时,华北地区降水偏少(偏多).数值试验表明,高原上潜热加热异常引起青藏高压、西太平洋副热带高压、亚洲季风以及欧亚中高纬地区环流的变化,进而影响到华北地区的降水.     

西宁和贵德盆地新生代沉积物重矿物变化对构造活动的响应

位于青藏高原东北缘的西宁、贵德盆地的新生代沉积序列较完整的记录了盆地周围物源区构造变形过程。重矿物是碎屑物质的重要组成部分,是最直观、有效揭示源区母岩、构造-沉积过程的重要手段。通过重矿物的系统分析,结合沉积-构造变形,揭示出始新世-上新世末西宁-贵得盆地及其源区经历了几个构造活动阶段:古新世-始新世早期的隆升阶段、始新世中期-渐新世晚期的构造稳定阶段、渐新世末-中新世初的构造隆升阶段、中中新世构造稳定阶段和晚中新世以来的强烈隆升阶段。并结合特征矿物（绿泥石）及古水流分析,推断古近纪西宁-贵德盆地是东昆仑山前一个统一盆地。中新世早期青藏高原的扩张导致了拉脊山开始隆起,使原型盆地解体;约8.5 Ma以来拉脊山强烈隆升,两侧盆地逐渐转变为山间盆地。这为正确理解青藏高原东北缘盆山格局的形成和演化提供了重要依据。     

四川九寨沟诺日朗瀑布钙华大坝放射性及沉积环境意义

以九寨沟诺日朗瀑布钙华大坝为研究对象,采用便携式手持伽马仪对大坝坝体进行放射性强度测量,同时对钙华大坝取样,进行主量、微量元素分析,以研究大坝形成的沉积环境.结果表明:大坝表层伽马辐射量平均值为27.57 Ur(当量铀),变异系数为18.73％;钙华主微量元素分析显示钙华颜色与本身所含的色素元素和矿物有关;大坝钙华(L...