Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

Nutrient maximums related to low oxygen concentrations in the southern Canada Basin

The phenomenon of nutrient maximums at 70～200 m occurred only in the region of the Canada Basin among the world oceans. The prevailing hypothesis was that the direct injection of the low-temperature high-nutrient brines from the Chukchi Sea shelf (＜50 m) in winter provided the nutrient maximums. However, we found that there are five problems in the direct injection process. Formerly Jin et al. considered that the formation of nutrient maximums can be a process of locally long-term regeneration. Here we propose a regeneration-mixture process. Data of temperature, salinity, oxygen and nutrients were collected at three stations in the southern Canada Basin during the summer 1999 cruise. We identified the cores of the surface, near-surface, potential temperature maximum waters and Arctic Bottom Water by the diagrams and vertical profiles of salinity, potential temperature, oxygen and nutrients. The historical 129Ⅰ data indicated that the surface and near-surface waters were Pacific-origin, but the waters below the potential temperature maximum core depth was Atlantic-origin. Along with the correlation of nutrient maximums and very low oxygen contents in the near-surface water, we hypothesize that, the putative organic matter was decomposed to inorganic nutrients; and the Pacific water was mixed with the Atlantic water in the transition zone. The idea of the regeneration-mixture process agrees with the historical observations of no apparent seasonal changes, the smooth nutrient profiles, the lowest saturation of CaCO3 above 400 m, low rate of CFC-11 ventilation and 3H-3He ages of 8～18 a around the nutrient maximum depths.     

酚羟基从乙酰基保护到苄基保护的一锅法转换

在Lemieux方法的基础上,将应用于糖类化合物的方法首次应用到酚类化合物上,报道了乙酰基保护的酚类化合物,在苄溴、氢氧化钾作用下于四氢呋喃中回流通过一锅转化法转换得到苄基保护化合物;在有多个乙酰基同时存在条件下,通过调节氢氧化钾的用量来控制苄基取代的程度。该方法操作简便,收率较高。     

产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究

从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验．实验结果证明,在以0．5％可溶性淀粉为碳源,以0．5％豆饼粉为氮 源,无机盐:0．424％NH4H2PO4、0．075％K2HPO4、0．02％MgSO4、0．5％CaCO3,起始 pH值8．5,接种36h种龄的种子10％,20℃、250r／min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高．最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22．4U／cm3和30．9U／cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景．     

3D Numerical Simulation of Overbank Flow in Non-Orthogonal Curvilinear Coordinates

1.Introduction Riverflowsinacompoundchannelofteninundatetheadjacentplainsathighdischarges.This generatesacomplicatedflowstructurebetweenthemainchannelflowandthefloodplainflow.In straightchannels,thevelocitydifferencebetweentheflowinthemainchannelandthatontheflood plainisoneofthemostdistinctivecharacteristics.Thisintroducesseveralphysicalandphenomenologi calfeaturesaroundthejunctionbetweenthemainchannelandthefloodplain,namely,theformation ofahighhorizontalshearlayerandstreamwiseandverticalvorti…     

粤港澳大湾区海洋经济创新发展研究

海洋经济创新发展对粤港澳大湾区创新驱动、打造具有国际竞争力的科技创新中心具有重要意义。文章介绍粤港澳大湾区当前的海洋经济创新发展情况及其优势和不足,从构建大湾区海洋创新发展产业链、驱动陆海科技创新一体化、完善科技创新驱动体制机制和推动科技金融服务蓝色经济发展等方面,提出粤港澳大湾区海洋经济创新发展的突破要点和路径。     

莺歌海盆地莺东斜坡带隐蔽油气藏类型及成藏主控因素

莺东斜坡带具有“两脊一洼”分段式展布之特殊区域地质构造格局,且自北而南“两脊一洼”三段式分布具有明显不同的构造及断裂活动特征与油气运聚规律,故不同区域区带隐蔽油气藏类型及成藏主控因素差异明显,其中,北段(即北延伸脊)成藏主控因素主要取决于烃源及油气运聚输导系统;中段(一洼即岭头断阶区)成藏主控因素则受控于烃源与岩性圈闭的有效性;南段(南延伸脊)成藏主控因素主要取决于地层岩性复合圈闭的有效性。根据不同类型隐蔽油气藏的成藏主控因素及分布规律,即可采取不同的研究方法及勘探技术对策,以期获得油气勘探的突破。     

天然热释光技术在南海东部表层沉积物油气潜力勘查中的应用

应用热释光技术对南海东部海域(13.00°~22.00°N、117.00°~119°30′E)42个站位的表层沉积物样品进行分析测量,结合气烃组合指标C1/(C2+C3)及甲烷碳同位素判别地表气烃的成因类型,并有效指示了海底深部油气藏散失引起的放射性异常,研究还发现热释光在不同沉积粒径中的测试剂量不同,结果为黏土质粉砂大于砂质粉砂;热释光也与部分化学元素含量有关,与稀土金属Nb、金属元素Zr呈显著正相关,与微量元素S、Ba呈负相关;天然热释光还与不同区块的沉积物酸解气烃甲烷、丙烷、异丁烷呈强烈正相关。数理统计分析结果表明沉积物的热释光和气烃的异常值完全吻合。     

三角帆蚌对水体悬浮物和叶绿素a消除量的研究

本研究在不同温度条件下分别测定了一龄(△1)和三龄(△3)三角帆蚌对水体中悬浮物和叶绿素a的消除量;研究了三角帆蚌对叶绿素消除效果与水体中藻类生物量之间的关系,并在池塘中挂养1200只三角帆蚌四个月。结果表明:三角帆蚌对水体中悬浮物和叶绿素a的消除率均随水温升高而提高,其中消除效果在总重量相同时△3>△1,较高水温条件下三角帆蚌在悬浮物中优先利用藻类;同一温度下,水体中藻类量增大时,三角帆蚌对叶绿素a的消除量增大而消除率却降低;在池塘挂养测试中与对照池塘比较,四个月后三角帆蚌因大量消除了池塘水中的悬浮物和叶绿素a,使池塘水体透明度提高从26cm提高到了80cm。     

盐田水体遥感分类方法研究

以连云港台北盐场为研究区，介绍了监督分类法和神经网络分类法及其在盐田水体遥感分类中的具体应用。研究结果表明，用神经网络分类法进行遥感影像自动分类，其分类精度高，显示了其在遥感领域较为广阔的应用前景。