GEOCHEMICAL CHARACTERISTICS OF INTERSTITIAL WATER OF THE BOHAI GULF

This paper discusses the geochemical characteristics of the interstitial water of the Bohai Gulf, where the main salt contents (K+, Na+, Ca++ Mg+ + , C1-, HCO3-, SO4-) in 55 sedimentary layers of 13 cores (3-4 m in length) and those of bottom seawater have been determined and the exchange capacity and exchange cations (K+, Na+, Ca++, Mg++) of the layers analysed (see Fig. 1)Fig. J. The stations of core samplings in the Bohai Gult.     

AN ECOLOGICAL STUDY OF BENTHIC ORGANISMS IN THE NEARSHORE WATERS TO THE WEST OF TAIWAN STRAIT

In seven cruises on benthic organisms in the nearshore waters(>50 m) to the west of the Taiwan Strait(23°20''-25°54''N,117°11''-119°58''E) in 1961-1964,altogether 189 stations of quantitative grabbing and 33 stations of qualitative trawling were made.
Altogether 392 species of benthic organisms have been identified.The composition was dominated by nearshore shallow water species,with tropical and subtropical species absolutely dominant.The total average of biomass was 38.0 g/m² and average density,116.0 ind./m².The communities of benthic organisms in the area can be classified into five communities namely,(1) Amphiura sp.-Acaudina molpadioides Community;(2) Protankyra asymmetrica-Arnphioplus pracstans-Murex trapa Community;(3) Modiolus meicalfei-Phyllophorus liuwutiensis-Armandia letocirris-Nephtys sinensis Community;(4) Branchiostoma belcheri-Solenr oseomaculatus-Marphysa sinensis- Onuphis eremita Community;(5) Iconomtra japonica-Barbatia decussata Community.     

AN ECOLOGICAL STUDY OF FOULING ORGANISMS IN XISHA ISLANDS

Monthly and seasonal panel experiments were carried out in the Yongxing Island from September 1979 to August 1981. Altogether 119 species of fouling organisms are collected, of which, Hydroides elegans, Serpula vermicular is, Scrupocellaria longispinosa, Amathia distant, Enteromorpha spp. and Ostrea spp. are dominant species. Fouling of organisms may occur all the year round with evident seasonal variations, Species succession in the process of community formation is very evident and the climax has arrived after two years. Great variation exists between fouling organisms in different years and at different stations.     

鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。     

斜坡提砌石护面厚度的计算

介绍了斜坡式防波堤砌石护面中的插砌条石和干砌块石在波浪作用下的力学图式及作用机理 ,重点给出考虑摩擦力作用时的砌石块体稳定厚度的计算公式 ,并结合工程实例验证了公式的适用性     

东营城区高精度三维地震采集方法研究

根据东营城区内建筑物较多，环境干扰较大，地下构造复杂，断裂非常发育，目的层埋藏深，资料信噪比较低的特点，以地震老资料和钻井资料为基础，建立地震地质模型，利用射线追踪技术选取合适的观测系统参数。充分利用卫星数字地图，没计了灵活多变的观测系统，采用可控震源与炸药震源配合使用方法，及时对城区内地下面元的覆盖次数、方位角和炮检距的分布进行质量监控，利用现场处理系统对资料进行及时分析，提高了地震资料品质。所获得的东营城区高精度三维地震采集资料显示，其浅中层分辨率和中深层信噪比都有了明显改善，取得了良好的地质效果。     

Study on copper complexing ligand concentrations in several China’s coastal waters

Copper complexing ligand concentrations in the Daya Bay, Qingdao coast, Jiaozhou Bay, South China Sea and Huanghe Estuary waters were determined by the anodic stripping voltammetry technique. The distribution regularity and the relationship with other parameters were discussed. The results were as follows: Copper complexing ligand concentrations of the South China Sea were a little higher than those of other sea areas, and they were apparently higher than those of the ocean. Compared with the subsurface layer (SSL) in the sea surface microlayer copper complexing ligand concentrations showed an enrichment phenomenon, of which the mechanism is similar to dissolved organic matter. The metal complexing ligand concentration profiles of the South China Sea showed that the value in the sea surface was the highest, then it decreased with depth accruing, and a higher value appeared at the bottom. Copper complexing ligand concentrations were higher than those of cadmium and lead. Ligands in each sea area exhibited a complicated property. In short, the distribution regularity of copper complexing ligand concentrations in China' s coastal waters was consistent with that of other regions in the world. Meanwhile, the positive relationship between the copper complexing ligand concentrations and biological oxygen demand, chemical oxygen demand, dissolved organic carbon, and viscosity were found clearly.     

假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因克隆与鉴定

采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp．cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明，该结构基因长819bp，与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61．5％，氨基酸同源性为56．2％。该基因已输入GenBank数据库，收录号AF348165。将该基因转化大肠杆菌，受体菌在含NaCl 1．0mol／L的培养基中甘油含量升高2．9倍，最终菌浓度提高3．6倍。可见这是一个与生物耐盐性相关的主基因，以其转化、培育耐盐农作物的前景十分光明。     

锯缘青蟹NAGase的催化机理的判断

以p-硝基苯酚-β-D-氨基葡萄糖苷（pNP-NAG）为底物，研究产物类似物：p-羧基苯酚和苯酚对青蟹N-乙酰-β-D-氨基葡萄糖苷酶（NAGase，Ec3．2．1．52）活力的影响．结果表明：p-羧基苯酚和苯酚对该酶有抑制的作用，IC50分别为20．0和100．0mmol／dm^3．p-羧基苯酚和苯酚对酶的抑制均表现为竞争性类型，其抑制常数K1分别为5．03和25．67mmol／dm^3．结合产物N-乙酰-β-D-氨基葡萄糖（NAG）抑制作用表现为反竞争性类型，判断NAGase水解pNP-NAG反应为有序双双反应（Ordered Bi Bi）．     

围垦的社会经济价值及其对海洋环境影响的评估

从围垦滩涂与保护湿地的关系着手,针对浙江省的滩涂围垦现状,讨论了沿海滩涂实施围垦工程所创造的社会经济价值,分析了湿地生态的环境特征及其对自然界的贡献,指出并探讨了海岸带资源开发与海岸工程建设所带来的问题,从湿地保护和经济可持续发展的角度提出了相关的建议。