哈维氏弧菌的生物学特性、流行病学及检测技术

哈维氏弧菌是1种革兰氏阴性、发光的海洋细菌,广泛分布于海洋环境中。它是最近10多年才被认识到的水产养殖动物的重要致病菌。文中综述哈维氏弧菌的生物学特性、流行病学、致病机制、密度感应系统、检测技术、疾病的防治及其应用。     

鱼类生长激素的异源表达、应用及安全性评价

鱼类生长激素对鱼的生长发育起着重要的调节作用,能促进鱼体快速生长,提高饵料转化率,在水产养殖业中具有重大应用价值。转生长激素基因鱼具有快速生长效应,可以缩短养殖周期,降低养殖成本和风险。鱼类生长激素基因异源表达体系的建立和发展,使获得大量廉价的鱼类生长激素产品成为可能,为鱼类养殖业新型饵料添加剂的研制开辟了新的途径。本文综述了转生长激素基因鱼和鱼类生长激素基因的异源表达两方面的研究及其安全性评价。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

陆面模式中土壤呼吸的研究概况

土壤呼吸是陆地植被吸收的CO2返回大气的基本途径,土壤呼吸速度轻微变化也会引起大气中CO2浓度的明显改变,进而影响气候变化。陆面过程模式中更好地描述土壤呼吸过程对于预测未来气候变化是至关重要的。对于土壤呼吸的模拟研究,介绍了经验模型和以过程为基础的机理模型,以及国内外陆面过程模式中土壤呼吸的研究情况,并讨论了土壤呼吸模式中需要改进的问题。       

天线罩疏水层老化对微波辐射计观测的影响分析

地基微波辐射计因天线罩疏水层老化,在雨天探测时天线罩上易形成积水从而造成探测性能的不稳定,是影响其在科研业务中广泛应用的关键问题之一。本研究利用2017—2018年北京地区6台微波辐射计降雨期间的观测数据,分析天线罩疏水层老化对其观测亮温的影响,结果显示随着天线罩使用时间的增长,尤其是使用3个月以后,降水时亮温增大的幅度明显变大,降水结束后亮温增大的持续时间也有增长趋势。同时,在北京南郊站开展的微波辐射计天线罩淋雨试验结果表明,天线罩使用40 d以后,随着天线罩污染老化,淋雨时观测亮温增大的幅度呈跳跃式增长,且淋雨结束后亮温仍然偏高,最长时间达2 h以上。因此,为提高微波辐射计观测数据的可用性,建议在实际使用过程中每季度更换一次天线罩,对于京津冀等重污染地区可根据实际情况适当提高更换频次。     

GPS高程测量在也门Block69中的应用分析

GPS高程测量代替常规水准测量的技术和方法已经成为这些年来许多大地测量学者们研究的热门课题之一,本文简单地介绍了GPS高程测量的模型及其适用范围,并通过在也门Block 69的应用分析,说明了在高程异常值变化平缓的平坦地区,只需测定少量（不少于3个）较均匀分布于测区的已知高程控制点,采用平面拟合法,拟合精度就能够达到测区测量精度的要求。     

基于GPS/CDMA集成的移动定位与服务技术

随着空间信息技术的发展,移动定位服务需要更高的定位精度。人们的活动范围越来越不确定,这给移动通信带来了无限商机和挑战,在此领域,日本处于世界领先地位,国内主要有Nokia、Ericsson、Motorola等。提出了一种基于GPS／CDMA集成的移动定位与服务技术,并分析了其中关键技术和实施的可行性。     

我们是怎样预报松潘7.2级地震的——纪念松潘7.2级地震30周年

回顾了1976年8月16日松潘7.2级地震中期、短期与临震预报,以及震后趋势判断的过程,简述了当年预报所依据的各类前兆异常。     

应用计算机进行固体矿产储量计算,提高地质工作水平

Windos XP版储量计算程序是作者90年代DOS版的全面升级。该程序按照目前地质工作普遍采用的公式和做法,符合现行规范规定和要求。程序对储量计算中矿体圈定等难点解决较好。该程序已经多个矿区应用检验,最近又为南非英铂公司采用推广。     

冀南綦村斑状石英二长岩角闪石环带结构及其地质意义

为阐释岩浆两阶段演化过程及其与铁成矿的关系,对邯邢铁矿区斑状石英二长岩中角闪石斑晶环带结构进行了成因矿物学研究.通过角闪石温度计、压力计、氧逸度计和湿度计的计算,发现岩浆在14km深处岩浆房内经过多次岩浆混合过程,其后演化的岩浆侵位到1.9 km的深度.岩浆房的温度为935～964℃,氧逸度为△NNO+0.27～△NN...