Isolation and characterisation of pristane metabolites formed in vivo by rainbow trout

Pristane (2,6,10,14 -tetramethylpentadecane) occurs ubiquitously in the marine environment. This hydrocarbon may be of biogenic or petrogenic origin.¹ Recently it has been shown that residual amounts of this branched alkane increased in marine organisms after an oil spill.^2,3 The lack of data on the fate of pristane in fish, added with the fact that this compound was considered by some authors as a non-metabolisable substance in vertebrates, including man,⁴ led us to investigate the capability of fish to metabolise pristane. In this study, urinary and fecal excretion, tissue distribution and metabolism of ³H-pristane were analysed in Salmo gairdneri R. after a single intragastric dose. In addition to unchanged hydrocarbon, various labelled compounds have been isolated and identified in liver, bile, faeces, urine and surrounding water, demonstrating that pristane was first oxidised to alcohols (pristanol and pristane-diol) and to acid (pristanic acid). The elimination of these compounds occurred in the form of conjugated products (primarily glucuronides) as well as free metabolites.     

JGOFS studies in the equatorial Pacific

This special issue is the third and final volume containing results from the JGOFS Process Study in the equatorial Pacific. Most of the Study in the equational Pacific. Most of the contributions evolved either from the US JGOFS workshop in 1994 on the equatorial Pacific in Scottsdale, AZ or from the NATO Advanced Research Workshop on the Carbon Cycle of the Equatorial Pacific in 1995 in Noumea, New Caledonia.     

Gravity Anomaly and Tectonics in the Northern Part of the North Fiji Basin - Preliminary Result of the 1994 NOFI Cruise under the NEW STARMER Programme

A surface ship gravity survey was carried out in the northern part of the North Fiji Basin during the NOFI cruise by the R/V l'Atalante in August-September, 1994. The two ridges inside the study area, the South Pandora Ridge and the Tripartite Ridge, present different structures and states of isostatic equilibrium in terms of gravity anomaly and its tectonic implications. The former is supported by a restoring force of an imaginary elastic plate in the crust and the latter by the Airy type isostasy. These characteristics can be derived from the difference in magmatic activity, as influenced by the difference in lithospheric structure. The latter is characterised by greater active magmatism and hydrothermalism underneath the ridge than the former. Such a difference in the magmatic activity and the horizontal scale of the shallow subsurface structure is derived from the difference in the stiffness or viscosity of the lithosphere beneath the two ridges.     

Automatic Registration of TOBI Side-Scan Sonar and Multi-Beam Bathymetry Images for Improved Data Fusion

Deep towed side-scan sonar vehicles such as TOBI acquire high quality imagery of the seafloor with very high spatial resolution but poor locational accuracy. Fusion of the side-scan sonar data with bathymetry data from an independent source is often desirable to reduce ambiguity in geological interpretations, to aid in slant-range correction and to enhance seafloor representation. The main obstacle to fusion is accurate registration of the two datasets.The application of hierarchical chamfer matching to the registration of TOBI side-scan sonar images and multi-beam swath bathymetry is described. This matches low level features such as edges in the TOBI image, with corresponding features in a synthetic TOBI image created by simulating the flight of the TOBI vehicle through the bathymetry. The method is completely automatic, relatively fast and robust, and much easier than manual registration. It allows accurate positioning of the TOBI vehicle, enhancing its usefulness as a research tool. The method is illustrated by automatic registration of TOBI and multi-beam bathymetry data from the Mid-Atlantic Ridge.     

Open sea berths for liquid bulk carriers

The transfer of liquid bulk through flexible transfer lines can be accomplished despite relatively large ship motions. For this reason, open sea berth terminals have replaced conventional harbors in many places. The thorough understanding of moored ships dynamics when subjected to waves, wind and currents, combined with the use of flexible dolphins, flexible fenders, asymmetric constant-tension winches and nylon tails permit us to design open sea berth terminals for relatively exposed areas. The design of an integrated system of marine sensors to continuously monitor all the critical elements-ship motion and mooring and fender forces-will enhance the operational procedure whilst insuring safe operations. A berth code can then be established for insuring the maximum efficiency of the terminal on the basis of the measurement of these critical elements.     

东海黑潮及琉球群岛以东海流研究进展

东海是西太平洋的一个边缘海,它西部有广阔的大陆架,东面以九州岛、琉球群岛(由奄美诸岛、冲绳诸岛、先岛诸岛组成)和台湾岛连线为界,具有较深的海槽。黑潮是一支高温、高盐、高流速的西边界流,它起源于菲律宾以东海域、流经台湾东岸进入东海,沿东海大陆架外缘向东北向流动，穿越吐噶喇海峡返回太平洋。通常的把自台湾东北端起至吐噶喇海峡一段称为东海黑潮。此外,在琉球群岛以东海域也常年存在一支稳定的西边界流,称为琉球海流。 对于东海黑潮和琉球海流的硏究,主要起步于20世纪80年代,通过进行中日联合调查研究、中日副热带环流调查研究等,取得了许多重要成果,苏纪兰(1994)、袁耀初(2000，Yuan,2006)、管秉贤(2002)等人都做过评述。本文基于以上研究评述,主要叙述200年以来对东海黑潮及琉球海流的硏究新进展。     

Active spreading and hydrothermalism in North Fiji Basin (SW Pacific). Results of Japanese French Cruise Kaiyo 87

The aim of the Japanese-French Kaiyo 87 cruise was the study of the spreading axis in the North Fiji Basin (SW Pacific). A Seabeam and geophysical survey allowed us to define the detailed structure of the active NS spreading axis between 16° and 22° S and its relationships with the left lateral motion of the North Fiji Fracture Zone. Between 21° S and 18°10′ S, the spreading axis trends NS. From 18°10 S to 16°40 S the orientation of the spreading axis changes from NS to 015°. North of 16°40′ S the spreading axis trends 160°. These two 015° and 160° branches converge with the left lateral North Fiji fracture zone around 16°40′ S to define an RRFZ triple junction. Water sampling, dredging and photo TV deep towing give new information concerning the hydrothermal activity along the spreading axis. The discovery of hydrothermal deposits associated with living communities confirms this activity.     

Authigenic carbonates from methane seeps of the Congo deep-sea fan

Submersible investigations with the ROV Victor 6000 of some pockmark structures on the seafloor of the Congo deep-sea fan have shown that they are active venting sites of methane-rich fluids, associated with abundant fauna and carbonate crusts. Moreover, methane hydrates have been observed both outcropping and deep in the sediments in the centre of the “Regab” giant pockmark. Authigenic carbonates, mostly calcite sometimes mixed with aragonite, are cementing the sedimentary matrix components and fauna; diatoms are abundant but only as moulds, indicating that biogenic silica dissolution occurred in situ synchronous with carbonate precipitation. The occurrence of diagenetic barite and pyrite in some carbonate crusts demonstrates that they can be formed either within the sulphate/methane transition zone or deeper in sulphate-depleted sediments. The oxygen isotopic compositions of the diagenetic carbonates (3.17–6.01‰ V-PDB) indicate that precipitation occurred with bottom seawater mixed with a variable contribution of water from gas hydrate decomposition. The very low carbon isotopic compositions of the diagenetic carbonates (−57.1 to −27.75‰ V-PDB) demonstrate that carbon derives mostly from the microbial oxidation of methane.     

Experimental survey of the hydrodynamic performance of a small spar buoy

The objective in this experimental work is to evaluate the capability of several cylindrical buoys to follow and measure waves. Eleven configurations have been studied, eight among which were furnished with disks of different diameters at the waterline. The experiments took place in the test basin at ISITV. On the one hand, we have obtained a set of transfer coefficients for each frequency and each configuration in regular waves. On the other hand, we have determined the corresponding transfer functions, then we have used the latter to measure irregular waves. The time signals as measured by the buoys with and without corrections have been compared with wave gage measurements and subjected to a wave-by-wave analysis. The results allow the conclusion that the buoy with a medium size disk constitutes the best compromise. In fact, it permits the buoy to follow the free surface while minimizing parasitic motions.