Growth and succession patterns of major phylogenetic groups of marine bacteria during a mesocosm diatom bloom

Our objective was to track microbial processes associated with serial degradation of organic matter derived from algal blooms. To do this, we analyzed population fluctuations and growth responses of major phylogenetic groups of free-living marine bacteria. We used bromodeoxyuridine immunocytochemistry–fluorescence in situ hybridization methodology to examine marine bacterial community development during and after a diatom bloom in a mesocosm. We revealed that the Roseobacter/Rhodobacter, SAR11, Alteromonas, and Bacteroidetes groups were clearly major phylotypes responsible for most free-living bacterial biomass and production throughout the experiment. The clearest bacterial response was a proliferation of the Alteromonas group (cells with large volumes) during development of the bloom (up to 30?% of actively growing cells). Populations of these bacteria declined sharply thereafter, likely due to grazing. Alteromonas group responses suggest that these bacteria strongly influenced the flux of organic matter at an early bloom stage. The growth potential of Bacteroidetes was relatively large as the bloom peaked; this early development probably contributed to the initial stage of bloom decomposition. In contrast, the contribution of Roseobacter/Rhodobacter to total bacterial production increased at a late stage of decomposing of the bloom. The contributions of Betaproteobacteria, SAR11, and SAR86 groups to total bacterial abundance and production were relatively minor throughout the experiment. These results imply that the ability to utilize organic matter derived from diatoms varies among bacterial phylotypes, and, frequently, less abundant but ecological specialist taxa such as Alteromonas may play major roles in the flux of organic matter during diatom blooms.     

Sound velocity measurements in dhcp-FeH up to 70 GPa with inelastic X-ray scattering: Implications for the composition of the Earth's core

We have determined the density evolution of the sound velocity of dhcp-FeH_x (x ≈ 1) up to 70 GPa at room temperature, by inelastic X-ray scattering and by X-ray diffraction. We find that the variation of V_P with density is different for the ferromagnetic and nonmagnetic dhcp-FeH_x, and that only nonmagnetic dhcp-FeH_x follows Birch's law. Combining our results with Birch's law for iron and assuming an ideal two-component mixing model, we obtain an upper bound of the hydrogen content in the Earth's inner core, 0.23(6) wt.% H, corresponding to FeH_0.13(3). The iron alloy with 0.23(6) wt.% H can satisfy the density, and compressional and shear sound velocities of the PREM inner core, assuming that there are no other light elements in the inner core.     

Short-term flow fluctuations in Onagawa Bay,Japan, as revealed by long-term mooring observation

Short-term flow fluctuations in the southern central part of Onagawa Bay were examined using long-term mooring and hydrographic data observed during the period from May 2013 to April 2014. The short-term flow fluctuations were dominant in the periodic bands of 15–27 days and shorter than 10 days. The principal and minor components of these flow fluctuations were respectively along and across the local isobaths, which are almost parallel in the north direction. The northward flow fluctuations along the local isobaths were correlated with the northeastward wind fluctuations in both periodic bands, and these correlations were more evident from fall to winter. On the basis of these results, the northward flows are regarded as wind-induced barotropic coastal jets. On other hand, the eastward flow fluctuations across the local isobaths were related to inflow and outflow via the bay mouth or the Izushima Channel. Inflow and outflow with reverse flow at lower depths formed in summer, but those with vertically uniform flow tended to form in winter. The main summertime inflow and outflow were driven by horizontal gradients in density. These permit us to regard the main summertime inflow and outflow as gravitational circulation. Also, the summertime inflow can be intermittently caused by internal waves.     

Simultaneous measurements of compressional wave and shear wave velocities, Poisson's ratio, and Vp/Vs under deep crustal pressure and temperature conditions: Example of silicified pelitic schist from Ryoke Belt, Southwest Japan

The ultrasonic technique for measuring travel times of compressional and shear waves using dual-mode transducers was adapted to a piston cylinder apparatus, allowing simultaneous measurements of travel times of compressional and shear waves of island arc samples under the high pressure and temperature conditions of island arcs. This method enables us to determine elastic properties and their pressure and temperature derivatives simultaneously. Furthermore, Vp/Vs can be directly determined from travel times of compressional and shear waves independently of length change due to compression or thermal expansion of rock samples under deep crustal conditions, providing more accurate Vp/Vs values than those determined from individual measurements of travel times of both elastic wave types using single-mode transducers. Experimental techniques and results are demonstrated using data on silicified pelitic schist from the Ryoke Belt to 0.6 GPa. The simultaneous measurement gives Vp  = 5.60 km/s, ∂ Vp /∂ P  = 0.090 (km/s)/GPa, Vs  = 3.37 km/s, ∂ Vs /∂ P  = 0.05 (km/s)/GPa, σ  = 0.216, and Vp / Vs  = 1.66 at ambient conditions. The temperature derivatives were constrained from fitting using linear functions of temperature, yielding ∂ Vp /∂ T  = −0.518 × 10⁻³ (km/s)/K and ∂ Vs /∂ T  = −0.182 × 10⁻³ (km/s)/K. Performing simultaneous measurements of travel times of compressional and shear waves using dual-mode transducers, it is possible to accurately determine Vp / Vs and Poisson's ratio of crustal minerals and rocks at deep crustal conditions to study the composition of the crustal interior, e.g. rock types and fluids below the hypocentral region of earthquakes or around bright spots.     

Sound velocity measurement in liquid water up to 25 GPa and 900 K: Implications for densities of water at lower mantle conditions

We extended the pressure range of sound velocity measurements for liquid water to 25 GPa and 900 K along the melting curve using a laser heated diamond anvil cell with a combined system of Brillouin scattering and synchrotron X-ray diffraction. Experimental pressure and temperature were obtained by solving simultaneous equations: the melting curve of ice and the equation of state for gold. The sound velocities obtained in liquid water at high pressures and melting temperatures were converted to density using Murnaghan's equation of state by fitting a parameter of the pressure derivative of bulk modulus at 1 GPa. The results are in good agreement with the values predicted by a previously reported equation of state for water based on sound velocity measurements. The equation of state for water obtained in this study could be applicable to water released by dehydration reactions of dense hydrous magnesium silicate phases in cold subducting slabs at lower mantle conditions, although the validity of Murnaghan's equation of state for water should be evaluated in a wider pressure and temperature ranges. The present velocity data provides the basis for future improvement of the accurate thermodynamic model for water at high pressures.     

Crustal structure beneath Aso Caldera,Southwest Japan,as derived from receiver function analysis

Aso Volcano experienced a huge pyroclastic eruption 90 thousand years ago, and formed a large caldera (18 km × 25 km). In order to test the hypothesis of a magma body in the mid and lower crust that has been suggested geophysically and geochemically, we investigated seismic velocity discontinuities and velocity structure beneath Aso Caldera using receiver functions and a genetic algorithm inversion. We confirm the existence of the Moho at depths between 30 km and 35 km and a large velocity anomaly should exist in the deep portion of the crust beneath Aso Caldera, from imaging of receiver functions observed only at stations outside the caldera. As a result of a more detailed examination with GA inversion, a low velocity layer is detected at depths between 10 km and 24 km beneath the western part of the caldera. S-wave velocity of the layer is estimated to be 2.0–2.4 km/s. We estimate that the low velocity layer contains at most 15% melt or 30% aqueous fluid. The layer exists near the Conrad and at the same depths as the swarm of the low frequency earthquakes and a compressional and dilatational deformation source which are expected to be caused by fluid movement beneath the middle-eastern part of the caldera. Fluid contained in the layer might be related with huge pyroclastic eruptions of Aso Volcano.     

Zinc contamination in river water and sediments at Taisyu Zn–Pb mine area,Tsushima Island,Japan

Tsushima Island is one of the oldest zinc-lead mining areas in Japan. River water and sediment samples were collected mainly from Taishu area to determine the contamination level of Zn and to clarify its behaviour in the natural system. Among the water samples analysed, 64% exceeded the standard environmental limit of 0.03 µg ml^− 1 for Zn. In most cases, Zn concentration in sediment samples also exceeded the standard value, and the concentration varied from 86.75–7490.07 µg g^− 1. The mineralogical constituents in sediments were almost similar and quartz had the strongest peak, but the interior part of the ores had many minerals, with galena having the highest proportion. Considering the enrichment factor values (EFc), 12 samples have values of more than 50, indicating a high pollution load for Zn. This study revealed that the sulphide ores, and contaminated sediments, are the possible contamination sources of Shiine River, and Zn dissolution occurred by reactions, such as desorption and ion exchange.     

Miocene provenance change in Himalayan foreland basin and Bengal Fan sediments,with special reference to detrital garnet chemistry

Bengal Fan Miocene sediments were collected during International Ocean Discovery Program Expedition 354 and investigated using petrographic and detrital garnet chemistry analyses. The Miocene Siwalik Group, which is composed of sediments deposited in the Himalayan foreland basin, was also analyzed for comparison with the Bengal Fan data for the provenance change during the Miocene. Our petrographic analyses revealed that the Miocene sediments of the Bengal Fan and Siwalik Group consist predominantly of Higher Himalayan Crystalline (HHC)-derived detritus such as chloritoid, staurolite, sillimanite, and/or kyanite, which appear among the accessory minerals. The chemistry of the detrital garnet varies across the stratigraphy; most of the garnet is rich in almandine and poor in spessartine and pyrope. However, pyrope-rich garnet, which is considered to originate from the HHC core (granulite facies), was found in the lower to upper Miocene deposits. The deposition of HHC-derived detrital garnet began before the Middle Miocene (15 Ma) and before the Late Miocene (10–9 Ma) in the Siwalik Group. The Bengal Fan data, by contrast, indicated that pyrope-rich garnet appeared in the Early Miocene (17.3 Ma) and Late Miocene (8.5–6.5 Ma). We conclude that the Bengal Fan sediments record the erosion of the HHC zone since the Early Miocene that appears in the Siwalik sediments. Furthermore, we found that the HHC-derived inputs decreased from the late Middle Miocene (12 Ma) to the early Middle Miocene (10 Ma) in both the Nepal Himalaya foreland basin and the Bengal Fan. The disappearance of the HHC-derived detritus is probably the result of dilution by Lesser Himalayan detritus, which suggests that the Lesser Himalayan zone, which is composed of metamorphosed and unmetamorphosed sedimentary rocks, was uplifted.     

Estimation of groundwater residence time using the 36Cl bomb pulse

We propose a methodology for estimating the residence time of groundwater based on bomb-produced (36)Cl. Water samples were collected from 28 springs and 2 flowing wells located around Mt. Fuji, Central Japan. (36)Cl/Cl ratios in the water samples, determined by accelerator mass spectrometry (AMS), were between 43 × 10(-15) and 412 × 10(-15). A reference time series of the above-background (i.e., bomb-derived) (36)Cl concentration was constructed by linearly scaling the background-corrected Dye-3 data according to the estimated total bomb-produced (36)Cl fallout in the Mt. Fuji area. Assuming piston flow transport, estimates of residence time were obtained by comparing the measured bomb-derived (36)Cl concentrations in spring water with the reference curve. The distribution of (36)Cl-based residence times is basically consistent with that of tritium-based estimates calculated from data presented in previous studies, although the estimated residence times differ between the two tracers. This discrepancy may reflect chlorine recycling via vegetation or the relatively small change in fallout rate, approximately since 1975, which would give rise to large uncertainties in (36)Cl-based estimates of recharge for the period, approximately since 1975. Given the estimated ages for groundwater from flowing wells, dating based on a (36)Cl bomb pulse may be more reliable and sensitive for groundwater recharged before 1975, back as far as the mid-1950s.