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61.
Hydroelastic effect of sloshing is studied through an experimental investigation. Different excitation frequencies are considered with low-fill-depth and large amplitude. Morlet wavelet transform is introduced to analyze the free surface elevations and sloshing pressures. It focuses on variations and distributions of the wavelet energy in elastic tanks. The evolutions of theoretical and experimental wavelet spectra are discussed and the corresponding Fourier spectrums are compared. Afterwards, average values of the wavelet spectra are extracted to do a quantitative study at various points. From the wavelet analysis, sloshing energies are mainly distributed around the external excitation frequency and expanded to high frequencies under violent condition. In resonance, experimental wavelet energy of the elevation in elastic tanks is obviously less than that in the rigid one; for sloshing pressures, the elastic wavelet energy close to the rigid one and conspicuous impulse is observed. It recommends engineers to concern the primary natural frequency and impulsive peak pressures.  相似文献   
62.
2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。  相似文献   
63.
This paper examines spatial and temporal variations of mesozooplankton abundance, biomass and community structure during three cruises of July 2002 (summer), January 2003 (winter), and April 2003 (spring) in the Pearl River estuary, China. Zooplankton abundance and biomass fluctuated widely and showed distinct heterogeneity in the Pearl River estuary. A total of 154 species were identified during three surveys. The number of zooplankton species richness was strongly linked to salinity. Hierarchical cluster analysis identified three zooplankton groups during this study. Estuarine, neritic and pelagic groups corresponded to the upper, middle and lower reaches in the Pearl River estuary. The difference among groups could be mainly ascribed to changes in the relative contributions of the dominant species. The fluctuations in the zooplankton abundance, biomass and community structure were determined by the interactive effects of freshwater inflow, tidal and coastal currents, chlorophyll a, salinity and temperature. Significant spatial variability in the distribution of zooplankton species, abundance and biomass can be ascribed to the virtual presence of a horizontal gradient in salinity.  相似文献   
64.
曹妃甸填海工程初期规划有明显缺陷,填海面积过大,尤其是阻断了浅滩潮道(纳潮河),对海洋环境、老龙沟港口潜力区有明显损害。作者的积极建议和相关论文的发表促成了2007年曹妃甸新填海规划的出台,其中保留浅滩潮道(即开通纳潮河)和在老龙沟上游修建大港池是新规划的主要改进内容。阻断浅滩潮道后曹妃甸区域的地形地貌及水动力变化证实了作者观点的正确性。纳潮河于2016年8月开通。纳潮河的开通,可大大提升曹妃甸景观,增加约40 km不淤积的优良海岸线,部分恢复原海洋潮流系统和保护海洋环境,促进海水的循环,并使曹妃甸的几个港池不再淤积或淤积变轻。  相似文献   
65.
Simplified techniques based on in situ testing methods are commonly used to predict liquefaction potential. Many of these simplified methods are based on finding the liquefaction boundary separating two categories (the occurrence or non-occurrence of liquefaction) through the analysis of liquefaction case histories. As the liquefaction classification problem is highly nonlinear in nature, it is difficult to develop a comprehensive model taking into account all the independent variables, such as the seismic and soil properties, using conventional modeling techniques. Hence, in many of the conventional methods that have been proposed, simplified assumptions have been made. In this study, an updated support vector machine (SVM) based on particle swarm optimization (PSO) is used to evaluate liquefaction potential in two separate case studies. One case is based on standard penetration test (SPT) data and the other is based on cone penetration test (CPT) data. The SVM model effectively explores the relationship between the independent and dependent variables without any assumptions about the relationship between the various variables. This study serves to demonstrate that the SVM can “discover” the intrinsic relationship between the seismic and soil parameters and the liquefaction potential. Comparisons indicate that the SVM models perform far better than the conventional methods in predicting the occurrence or non-occurrence of liquefaction.  相似文献   
66.
The time-dependent feature of soft soils has gained intensive attraction in recent years. Due to the high water content and viscous property, the Hong Kong marine deposit (HKMD) frequently poses a challenge to geotechnical practice, particularly to the reclamation in Hong Kong. A key issue related to reclamation design, foundation construction and maintenance is excess settlement/deformation of the ground. Formation of HKMD typically goes through sedimentation and self-weight consolidation. A series of one-dimensional settling column consolidation tests, together with conventional oedometer tests were conducted on the HKMD. By normalizing parameter in settling stage, a unique global relationship of effective stress, σz′ and strain, ?z could be established. Results reveal that the settling curve and settling rate are significantly affected by the sediment concentration in self-weight consolidation. After the primary self-weight consolidation, the settling rate is reduced in the “secondary self-weight consolidation.”  相似文献   
67.
草鱼出血病病毒VP7蛋白基因的人工合成与原核表达   总被引:2,自引:0,他引:2  
根据GenBank已发表的GCRVVP7蛋白基因序列,设计合成2条特异性引物和19条搭桥引物,通过搭桥PCR人工合成带有3处变异碱基的GCRVVP7蛋白基因整个编码框,经过酶切、连接、PCR鉴定和测序鉴定克隆到原核表达载体pColdTF中,并转化大肠杆菌BL21(DE3),进行IPTG诱导表达和表达产物的SDS-PAGE、Western-blotting分析、纯化以及反应原性的iELISA检测。结果表明,成功人工合成了长831bp的GCRV VP7蛋白基因编码框和构建了VP7蛋白基因的重组质粒pCold TF-VP7,pColdTF-VP7转化菌经IPTG(1mmol/L)诱导3—4h即可获得特异性蛋白VP7重组蛋白的高效表达,VP7重组蛋白相对分子量大小约为73.9ku,与预期大小相符,且与鼠抗GCRV血清有良好反应原性。  相似文献   
68.
This article presents results from a series of Ko-consolidated compression and extension triaxial tests on specimens from undisturbed samples of Hong Kong Marine Deposits (HKMD). To investigate the strain-rate effects, a total of seven Ko-consolidated triaxial tests were conducted including four compression tests and three extension tests. After Ko-consolidation, the triaxial test specimens were sheared at step-changed axial strain rates under three different confining pressures of 50 kPa, 150 kPa, and 400 kPa, respectively. The step-changed strain rates were applied in the following order: +2%/h, +0.2%/h, +20%/h, -2%/h (unloading) and +2%/h (reloading) for the four compression tests and -2%/h, -0.2%/h, -20%/h, +2%/h (unloading) and -2%/h (reloading) for the three extension tests. The results are reported and analyzed in the paper. The results show that the strain rate effects, the stress-strain characteristics, and the effective stress paths of the specimens for tests in a compression state are different from those for tests in an extension stage. One order of magnitude increase in axial strain rate causes an average 8.6% increase in undrained shear strength for compression tests and a 12.1% increase for extension tests. It is also found that the failure mode of the specimens in compression is different from that in extension. The stress-strain behavior of specimens shows strain-softening and a clear shear band in compression tests, but strain-hardening without any clear shear band in extension tests for the same absolute value of axial strain.  相似文献   
69.
This article presents a case history of determination of effective depth of prefabricated vertical drains (PVDs) under embankment loading on a very soft clay deposit in central China, near Jiujiang, Jiangxi Province. The height of the embankment was 5.3 m and construction time was about one year. The PVDs were installed to a depth of 8.5 m at a spacing of 1.5 m in a triangular pattern. Field observations and the finite element method (FEM) were employed to analyze the performance of the soft deposit during embankment construction. The influential depth of the embankment loading was evaluated based on settlement, excess pore pressure, and stress increase in subsoil, both from the observed data and FEM analysis. The effective PVD depth was determined in the following ways: (1) the depth of 5% subsoil settlement of surface settlement; (2) vertical stress increase in subsoil of 25% in-situ stress; and (3) consolidation time/PVD depth relation by FEM. Based on the analysis, the effective depth of PVDs was determined to be between 10 and 12.8 m for this field case.  相似文献   
70.
贾朋群  张云荣  殷华梅 《气象》2000,26(8):53-56
试图提出一个由中美双方大气科学家参与的虚拟合作实验室的解决方案,实验室包括虚拟资料/信息系统、虚拟模拟系统、虚拟应用分析系统、虚拟培训学校、虚拟图书馆、虚拟可视化实验室和虚拟出版系统等。  相似文献   
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