大头鳕和半滑舌鳎RAPD分析条件的优化

大头鳕和半滑舌鳎是我国重要的两种经济鱼类,本论文以大头鳕和半滑舌鳎基因组DNA为模板,对RAPD分析中的DNA模板浓度进行了优化,确定最适浓度。并分别筛选出20条和18条扩增结果稳定性好、多态性强的随机引物用于大头鳕以及半滑舌鳎的RAPD遗传变异分析。     

Downward flux of zooplankton fecal pellets in the upper 2,000 m water column of the Izu Trench,western North Pacific

Sinking particulate matter were obtained from twelve depths using free-drifting sediment trap arrays which were deployed in the upper 2,000 m water column of the Izu Trench, northwest Pacific Ocean. The largest flux of 146 mgC m^–2 day^–1 was observed at 150 m depth. The flux generally decreased with depth below the maximum, however, minor flux peaks occurred at 1,000 and 1,250 m depth (>30 mgC m^–2 day^–1). Sinking large particles (>100 µm) were composed of fecal pellets typical of crustaceans, macroscopic aggregates, and planktonic organisms and their fragments. Three major components constituted 19%, 20% and 29%, respectively, of the total carbon flux (averaged from the fluxes at five depths; 50, 100, 150, 1,000 and 2,000 m). Among them, fecal pellet flux and large organism flux were well correlated with the total flux. The close correspondence between the fecal flux and the total carbon flux suggests that the latter is derived from a group of variables including other biogenic matter, among which fecal pellet is one of the leading factors controlling total flux, though the latter is only a minor covariable in quantity. Vertical flux profiles of fecal pellets and their internal constituents revealed some new inputs of feces occurring through the water column. This phenomenon implies that downward transportation of organic material is characterized by feeding and egestion activities of zooplankton, including overlapping processes of sinking and dispersion of large fecal particles and repackaging of dispersed small particles.     

Feeding Habits of the Mesopelagic Fish Gonostoma gracile in the Northwestern North Pacific

The diet of Gonostoma gracile, a numerically abundant mesopelagic fish in the Subtropical Region and the Transition Domain of the northwestern North Pacific, was examined using 520 specimens collected during June–July 1988, June 1995 and November 1995. The prey included mainly copepods, ostracods, amphipods and euphausiids. Copepods and ostracods were the most abundant, comprising approximately 70% of the total diet. There was little evidence of an ontogenetic dietary shift; Pleuromamma copepods were the most abundant prey for all size classes of fish ranging from 19 to 116 mm in standard length. The size range of prey increased with growth, but all fish sizes examined fed mainly on 1–4 mm long prey. Luminescent copepods and ostracods were the most abundant prey, suggesting that G. gracile detects its prey visually. This revised version was published online in August 2006 with corrections to the Cover Date.     

Geochronological and petrological investigations of Miocene felsic igneous rocks in the Amakusa Islands,southwest Japan: Possible extension of the Setouchi Volcanic Belt

The opening of the Japan Sea led to the separation of southwest Japan from the Eurasian continent. Subsequent to this event, a diverse range of igneous activities occurred in southwest Japan. On the back-arc side of the region, igneous activity commenced at approximately 22 Ma and persisted for an extended period. In the trench-proximal region of southwest Japan, magmatism initiated around 15.6 Ma, immediately following the cessation of the Japan Sea opening, in correlation with the subduction of the Philippine Sea plate beneath southwest Japan. The Amakusa Islands in western Kyushu host felsic to intermediate igneous rocks with Miocene radiometric ages. There has been a debate regarding the attribution of the igneous rocks in Amakusa Island among the Miocene igneous rocks in southwest Japan. To address this issue, we conducted zircon U–Pb dating and analyzed the major- and trace-element compositions of felsic igneous rocks in the Amakusa Islands to elucidate their characteristics. The obtained U–Pb ages range from 14.5 to 14.8 Ma, suggesting contemporaneity between magmatism in the Amakusa Islands and the Setouchi Volcanic Rocks in the trench-proximal region of southwest Japan. The major and trace element compositions of the felsic igneous rocks exhibit similarities to the dacites of the Setouchi Volcanic Rocks. These findings support previous suggestions that the magmatism in the Amakusa Islands can be correlated with the Setouchi Volcanic Rocks, based on the discovery of a high-Mg andesite dike and paleo-stress analysis utilizing the direction of dikes and sills. Therefore, the Setouchi Volcanic Belt is proposed to extend further west than the previously identified Ohno volcanic rocks in eastern Kyushu. The subduction of the Shikoku Basin of the Philippine Sea plate toward western Kyushu supports the hypothesis that the Kyushu-Palau Ridge was positioned west of Kyushu at ~15 Ma.     

Does heavy oil pollution induce bacterial diseases in Japanese flounder Paralichthys olivaceus?

As basic research for the effect of heavy oil on the fish immune system, in this study, the number of leukocyte was counted in Japanese flounder Paralichthys olivaceus, after exposure to heavy oil at a concentration of 30 g/8L for 3 days. To compare the numbers of bacteria in the skin mucus between oil-exposed and control fish, viable bacteria were enumerated by counting colony forming unit (CFU). Compared with 5.79+/-1.88 x 10(7)leukocytes/mL in the controls, the exposed fish demonstrated higher counts, averaging 1.45+/-0.45 x 10(8)cells/mL. The bacterial numbers of control fish were 4.27+/-3.68 x 10(4)CFU/g, whereas they were 4.58+/-1.63 x 10(5)CFU/g in the exposed fish. The results suggest that immune suppression of the fish occurred due to heavy oil stressor, and bacteria could invade in the mucus, resulting in the increasing leukocyte number to prevent infectious disease.     

Determination of cation distribution in (Co,Ni,Zn)2SiO4 olivine by synchrotron X-ray diffraction

 The cation distribution of Co, Ni, and Zn between the M1 and M2 sites of a synthetic olivine was determined with a single-crystal diffraction method. The crystal data are (Co_0.377Ni_0.396Zn_0.227)₂SiO₄, M _r  = 212.692, orthorhombic, Pbnm, a = 475.64(3), b = 1022.83(8), and c = 596.96(6) pm, V = 0.2904(1) nm³, Z = 4, D _x  = 4.864 g cm⁻³, and F(0 0 0) = 408.62. Lattice, positional, and thermal parameters were determined with MoKα radiation; R = 0.025 for 1487 symmetry-independent reflections with F > 4σ(F). The site occupancies of Co, Ni, and Zn were determined with synchrotron radiation employing the anomalous dispersion effect of Co and Ni. The synchrotron radiation data include two sets of intensity data collected at 161.57 and 149.81 pm, which are about 1 pm longer than Co and Ni absorption edges, respectively. The R value was 0.022 for Co K edge data with 174 independent reflections, and 0.034 for Ni K edge data with 169 reflections. The occupancies are 0.334Co + 0.539Ni + 0.127Zn in the M1 sites, and 0.420Co + 0.253Ni + 0.327Zn in the M2 sites. The compilation of the cation distributions in olivines shows that the distributions depend on ionic radii and electronegativities of constituent cations, and that the partition coefficient can be estimated from the equation: ln [(A/B)_M1/(A/B)_M2] = −0.272 (IR _A -IR _B ) + 3.65 (EN _A −EN _B ), where IR (pm) and EN are ionic radius and electronegativity, respectively. Received: 8 April 1999 / Revised, accepted: 7 September 1999     

银离子和含银不锈钢的抗菌性研究

基于银因其具有良好的广谱抗茵活性和低毒性而在医学方面有着较为广泛的应用,对银离子(硝酸银)和含银不锈钢的抗茵活性进行了初步研究。实验结果表明,在10^6CFu／mL的初始接种浓度下,银离子对枯草杆菌、大肠杆菌和假单胞茵的最小抑茵质量浓度和最小杀菌质量浓度分别为2,6,8mg／L和2,8,10mg／L。大肠杆菌经8mg／L的银离子处理2h后,有99．9％以上的细菌被破坏。含银不锈钢也表现了较强的抗菌性能。在普通的、银合金和银涂层的sus304不锈钢表面附着的单层生物膜浸泡在PBS溶液中3d后,经CFDA-DAPI双染色和荧光显微镜观察计数获得的假单胞菌的平均存活率为98．0％,38．5％和15．1％。     

Regionality and seasonality of submesoscale and mesoscale turbulence in the North Pacific Ocean

The kinetic energy (KE) seasonality has been revealed by satellite altimeters in many oceanic regions. Question about the mechanisms that trigger this seasonality is still challenging. We address this question through the comparison of two numerical simulations. The first one, with a 1/10° horizontal grid spacing, 54 vertical levels, represents dynamics of physical scales larger than 50 km. The second one, with a 1/30° grid spacing, 100 vertical levels, takes into account the dynamics of physical scales down to 16 km. Comparison clearly emphasizes in the whole North Pacific Ocean, not only a significant KE increase by a factor up to three, but also the emergence of seasonal variability when the scale range 16–50 km (called submesoscales in this study) is taken into account. But the mechanisms explaining these KE changes display strong regional contrasts. In high KE regions, such the Kuroshio Extension and the western and eastern subtropics, frontal mixed-layer instabilities appear to be the main mechanism for the emergence of submesoscales in winter. Subsequent inverse kinetic energy cascade leads to the KE seasonality of larger scales. In other regions, in particular in subarctic regions, results suggest that the KE seasonality is principally produced by larger-scale instabilities with typical scales of 100 km and not so much by smaller-scale mixed-layer instabilities. Using arguments from geostrophic turbulence, the submesoscale impact in these regions is assumed to strengthen mesoscale eddies that become more coherent and not quickly dissipated, leading to a KE increase.     

Scarifying and fingering surfaces of plunging jets

The local surface deformation resulting from the oblique impact of a columnar water jet has been computed, using a three-dimensional large eddy simulation, as a model of the overturning jet of a breaking wave. The emergence of the secondary jet from the front face of the initial jet has been examined and the organisation of the vortices within the jet characterised. As the secondary jet emerges, the vorticity field becomes unstable under the action of the strong shear beneath the jet surface and pairs of longitudinal counter-rotating vortices stretched along the direction of the jet projection are formed. The presence of these longitudinal vortex pairs creates convergent surface flows, resulting in the formation of longitudinal scars on the rear face of the projecting jet. Following significant growth of the scars on both its upper and lower surfaces, the jet decouples into fingers. The lateral widths of the longitudinal vortices provide a minimum measure of the finger size. A horizontal Froude number Fr_h, representing a measure of strength of horizontal shear in a gravity-dominated impacting flow is defined, which characterises the organisation of the longitudinal vortices occurring in the shear flow, and the resultant formation of scars and fingers. For higher Fr_h, stronger longitudinal vortices and deeper scars are formed at longer lateral intervals, enhancing the fingering process during the splashing event. Fundamental features of material transport in the vicinity of the surface of jets (e.g. gas transfer across a sea surface) are related to the entrainment of surface fluid by the longitudinal vortices, and is thus also characterised by Fr_h.