Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…     

浙西北江山─绍兴断裂带构造演化特征

江山—绍兴断裂带是中国南方最重要的构造带之一。它是江南陆块与华夏陆块神功运动形成的碰撞拼贴带。它表现出明显的多期变形、构造叠加的特征：神功运动形成近东西向平卧褶皱；印支运动沿断裂带有向北西强烈的推覆挤压。断裂带不同时期的活动反映了不同特征的构造环境，形成不同的构造变形特征，并对两侧的地质历史具有明显的控制作用。     

阿吾拉勒山西段二叠纪火山岩组合与构造环境分析

阿吾拉勒山体西段发育了厚度巨大的下二叠统火山岩,这些火山岩均属碱性玄武岩系列,以钾质类型为主,钠质类型次之,岩浆演化呈现出跨越B型趋势。这种火山活动是在大陆地壳内部产生的,代表了陆内裂谷演化的早期阶段。在早二叠世末期,由于受到新源运动的影响,这种火山活动被终止了。     

AR（P）参数估计协方差方法与其改进型方法的对比研究

定性分析协方差方法及其改进型在ＡＲ（Ｐ）参数估计中的应用，提供了一种估计参数的计算机上机流程图，比较了协方差方法及其改进型谱估计结果。     

对地方电视台转播CCTV时延的测定

讲述了同步广播卫星电视时间信号的测量方法和测量结果，获得了CCTV1、CCTV2通过同一颗卫星（亚太－1A）转播的时延差值为16333μs，测量精度在10ns以内；CCTV2、CCTV4分别经两颗卫星（亚太－1A、亚洲－Ⅱ）转播，在陕西天文台卫星地面接收站时延差值为1644．20μs，精度为50μs，并分析了影响时延差值和精度的原因。同时测量了地方电视台转播亚太－1A的CCTV信号与直接接收亚太－1A的CCTV信号的时延差值，其测量精度为0．1μs。这些结果为利用同步广播卫星的电视信号进行高精度的时间服务提供了参考依据。     

CARBON DYNAMICS OF WETLAND IN THE SANJIANG PLAIN

1INTRODUCTIONWetlandsplayanimportant roleintheprocessofcar-bonstorage.Thetotalcarbonstoredindifferentkindsofwetlandsisabout15%-35%ofthetotalcarboninthegloballandsoils(POSTetal.,1982;GORHAM,1991).Inaddition,wetlandsaresignificantnaturalsources fortheatmospheric CH4 (MOORE,1994).It isestimatedthatabout110×1012gCH4 originates fromanaerobicdecompositioninthenaturalwetlands,CH4 emission fromthenaturalwetlandsis15%-30%oftheglobalCH4 emission andtheCH4 emission from thepeat land at hi…     

A Type IV Radio Burst Associated with Type III Burst,Pulsation and Spike Emissions

A type IV radio burst accompanied by several normal- and reverse-drifting type III bursts, multiple long-term quasi-periodic pulsations and spikes was observed with the radio spectrometers (1.0–2.0 and 2.6–3.8 GHz) at National Astronomical Observatories of China (NAOC) on 23 September 1998. In combination with the images of Siberian Solar Radio Telescope (SSRT) of Russia, the complex and multiple magnetic structures inferred from the radio bursts reveal the existence of both large-scale and small-scale magnetic structures. This event suggests that the geometries of coronal magnetic fields contain multiple discrete electron acceleration/injection sites at different heights, and extended open and closed magnetic field lines. It can be shown that the energetic electrons gain access to open, diverging and closed field lines thus producing different types of radio bursts. From the characteristics of position, polarization, dispersion and displacement of the sources, the model of the type IV event is supported, which involves synchrotron emission from the electrons confined by the rapid scattering through the interaction of hydromagnetic wave with particles.     

Bond rolling resistance and its effect on yielding of bonded granulates by DEM analyses

A discrete element modelling of bonded granulates and investigation on the bond effect on their behaviour are very important to geomechanics. This paper presents a two‐dimensional (2‐D) discrete element theory for bonded granulates with bond rolling resistance and provides a numerical investigation into the effect of bond rolling resistance on the yielding of bonded granulates. The model consists of mechanical contact models and equations governing the motion of bonded particles. The key point of the theory is that the assumption in the original bond contact model previously proposed by the authors (55th CSCE‐ASCE Conference, Hamilton, Ont., Canada, 2002; 313–320; J. Eng. Mech. (ASCE) 2005; 131 (11):1209–1213) that bonded particles are in contact at discrete points, is here replaced by a more reliable assumption that bonded particles are in contact over a width. By making the idealization that the bond contact width is continuously distributed with the normal/tangential basic elements (BE) (each BE is composed of spring, dashpot, bond, slider or divider), we establish a bond rolling contact model together with bond normal/tangential contact models, and also relate the governing equations to local equilibrium. Only one physical parameter β needs to be introduced in the theory in comparison to the original bond discrete element model. The model has been implemented into a 2‐D distinct element method code, NS2D. Using the NS2D, a total of 86 1‐D, constant stress ratio, and biaxial compressions tests have been carried out on the bonded granular samples of different densities, bonding strengths and rolling resistances. The numerical results show that: (i) the new theory predicts a larger internal friction angle, a larger yielding stress, more brittle behaviour and larger final broken contact ratio than the original bond model; (ii) the yielding stress increases nonlinearly with the increasing value of β, and (iii) the first‐yield curve (initiation of bond breakage), which define a zone of none bond breakage and which shape and size are affected by the material density, is amplified by the bond rolling resistance in analogous to that predicted by the original bond model. Copyright © 2006 John Wiley & Sons, Ltd.     

Parameters of the intense X-ray and gamma-ray radiation from the solar flare of May 20, 2002, as observed from the Coronas-F spacecraft

We consider temporal, spectral, and polarization parameters of the hard X-ray and gamma-ray radiation observed during the solar flare of May 20, 2002, in the course of experiments with the SONG and SPR-N instruments onboard the Coronas-F spacecraft. This flare is one of the most intense gamma-ray events among all of the bursts of solar hard electromagnetic radiation detected since the beginning of the Coronas-F operation (since July 31, 2001) and one of the few gamma-ray events observed during solar cycle 23. A simultaneous analysis of the Coronas-F and GOES data on solar thermal X-ray radiation suggests that, apart from heating due to currents of matter in the the flare region, impulsive heating due to the injection of energetic electrons took place during the near-limb flare S21E65 of May 20, 2002. These electrons produced intense hard X-ray and gamma-ray radiation. The spectrum of this radiation extends up to energies ≥7 MeV. Intense gamma-ray lines are virtually unobservable against the background of the nonthermal continuum. The polarization of the hard X-ray (20–100 keV) radiation was estimated to be ≤15–20%. No significant increase in the flux of energetic protons from the flare under consideration was found. At the same time, according to ACE data, the fluxes of energetic electrons in interplanetary space increased shortly (～25 min) after the flare.     

STUDY ON THE INTERACTION BETWEEN NDVI PROFILE AND THE GROWING STATUS OF CROPS

Daily and ten-day Normalized Difference Vegetation Index(NDVI) of crops were retrieved from meteorological statellite NOAA AVHRR images ,The temporal variations of the NDVI were analyzed during the whole growing season,and thus the principle of the interaction between NDIV profile and the growing status of crops was discussed,As a case in point,the relationship between integral NDVI and winter wheat yield of Henan Province in 1999 had been analyzed.By putting integral NDVI values of 60 sample counties into the winter wheat yield-integral NDVI coordination,scattering map was plotted. It demonstrated that integral NDVI had a close relation with winter wheat yield.These relation could be described with linear,cubic polynomial ,and exponential regression,and the cubic polynomial regression was the best way,In general ,NDVI reflects growing status of green vegetation ,so crop monitoring and crop yield estimation could be realized by using remote sensing technique on the basis of time serial NDVI data together with agriculture calendars.