Effects of seawater acidification on hydrolytic enzyme activities

We have investigated the effects of seawater acidification on the activities of leucine aminopeptidase (LAPase), β-glucosidase (BGase), phosphatase (P-ase), α-glucosidase (AGase), and lipase (L-ase), which are important promoters of degradation of marine organic matter, including proteins, carbohydrates, organic phosphorus compounds, and lipids. Seawater samples were collected from a eutrophic coastal area, from Tokyo Bay, and from oligotrophic pelagic waters outside the Kuroshio Current. Enzyme activities were measured using fluorogenic substrates added to the seawater samples, which were acidified from pH 8.2 to 5.6 by a chemical buffer. Spontaneous hydrolysis of the substrates was shown to be negligible in heat-inactivated control samples, thus validating our results. LAPase was the most sensitive to acidification; enzyme activity rapidly decreased from pH 8.2 to 7.8, corresponding to a realistic scenario of ocean acidification. L-ase activity also decreased with acidification. Activities of P-ase and BGase were relatively constant across the pH levels examined, suggesting that their activity is not appreciably influenced by acidification. The effect of acidification on P-ase activities differed between the coastal and semipelagic samples, and this was likely due to freshwater influence at the nearshore station. Because of the low activity of AGase in the sample, the effect of acidification on this enzyme could not be examined. The effects of acidification on enzyme activity appear to vary depending on enzyme type and location, but we conclude that acidification will cause changes in the cycling of organic matter in marine ecosystems, in particular to proteinous and lipid substances.     

Relationships between Kuroko volcanogenic massive sulfide (VMS) deposits, felsic volcanism, and island arc development in the northeast Honshu arc, Japan

The northeast (NE) Honshu arc was formed by three major volcano-tectonic events resulting from Late Cenozoic orogenic movement: continental margin volcanism (before 21?Ma), seafloor basaltic lava flows and subsequent bimodal volcanism accompanied by back-arc rifting (21 to 14?Ma), and felsic volcanism related to island arc uplift (12 to 2?Ma). Eight petrotectonic domains, parallel to the NE Honshu arc, were formed as a result of the eastward migration of volcanic activity with time. Major Kuroko volcanogenic massive sulfide (VMS) deposits are located within the eastern marginal rift zone (Kuroko rift) that formed in the final period of back-arc rifting (16 to 14?Ma). Volcanic activity in the NE Honshu arc is divided into six volcanic stages. The eruption volumes of volcanic rocks have gradually decreased from 4,600?km³ (per 1?my for a 200-km-long section along the arc) of basaltic lava flows in the back-arc spreading stage to 1,000?C2,000?km³ of bimodal hyaloclastites in the back-arc rift stage, and about 200?km³ of felsic pumice eruptions in the island arc stage. The Kuroko VMS deposits were formed at the time of abrupt decrease in the eruption volume and change in the mode of occurrence of the volcanic rocks during the final period of back-arc rifting. In the area of the Kuroko rift, felsic volcanism changed from aphyric or weakly plagioclase phyric (before 14?Ma), to quartz and plagioclase phyric with minor clinopyroxene (12 to 8?Ma), to hornblende phyric (after 8?Ma), and hornblende and biotite phyric (after 4?Ma). The Kuroko VMS deposits are closely related to the aphyric rhyolitic activity before 14?Ma. The rhyolite was generated at a relatively high temperature from a highly differentiated part of felsic magma seated at a relatively great depth and contains higher Nb, Ce, and Y contents than the post-Kuroko felsic volcanism. The Kuroko VMS deposits were formed within a specific tectonic setting, at a specific period, and associated with a particular volcanism of the arc evolution process. Therefore, detailed study of the evolutional process from rift opening to island arc tectonics is very important for the exploration of Kuroko-type VMS deposits.     

Throughfall partitioning by trees

Although we know that rainfall interception (the rain caught, stored, and evaporated from aboveground vegetative surfaces and ground litter) is affected by rain and throughfall drop size, what was unknown until now is the relative proportion of each throughfall type (free throughfall, splash throughfall, canopy drip) beneath coniferous and broadleaved trees. Based on a multinational data set of >120 million throughfall drops, we found that the type, number, and volume of throughfall drops are different between coniferous and broadleaved tree species, leaf states, and timing within rain events. Compared with leafed broadleaved trees, conifers had a lower percentage of canopy drip (51% vs. 69% with respect to total throughfall volume) and slightly smaller diameter splash throughfall and canopy drip. Canopy drip from leafless broadleaved trees consisted of fewer and smaller diameter drops (D_{50_DR}, 50th cumulative drop volume percentile for canopy drip, of 2.24 mm) than leafed broadleaved trees (D_{50_DR} of 4.32 mm). Canopy drip was much larger in diameter under woody drip points (D_{50_DR} of 5.92 mm) than leafed broadleaved trees. Based on throughfall volume, the percentage of canopy drip was significantly different between conifers, leafed broadleaved trees, leafless broadleaved trees, and woody surface drip points (p ranged from <0.001 to 0.005). These findings are partly attributable to differences in canopy structure and plant surface characteristics between plant functional types and canopy state (leaf, leafless), among other factors. Hence, our results demonstrating the importance of drop‐size‐dependent partitioning between coniferous and broadleaved tree species could be useful to those requiring more detailed information on throughfall fluxes to the forest floor.     

Spiral mode of standing accretion shock instability in core-collapse supernovae

The study of standing accretion shock instability (SASI) in core-collapse supernova cores has been done with three-dimensional (3D) computer simulations. Rotations with various perturbations were introduced from outer boundary of an initial steady accreting flow. We found that one or two armed spiral accreting flow onto the proto-neutron star (PNS) is formed inside the shock wave depending on perturbations. The linear growth of spiral modes are clearly diagnosed by the mode analysis of the shock surface, and the lower m modes grow quickly in the linear regime.     

Evaluation of plastic energy dissipation capacity of steel beams suffering ductile fracture under various loading histories

The energy dissipation capacity of a structure is a very important index that indicates the structural performance in energy‐based seismic design. This index depends greatly on the structural components that form the whole system. Owing to the wide use of the strong‐column weak‐beam strength hierarchy where steel beams dissipate the majority of earthquake input energy to the structures, it is necessary to evaluate the energy dissipation capacity of the beams. Under cyclic loadings such as seismic effects, the damage of the beams accumulates. Therefore, loading history is known to be the most pivotal factor influencing the deformation capacity and energy dissipation capacity of the beams. Seismic loadings with significantly different characteristics are applied to structural beams during different types of earthquakes and there is no unique appropriate loading protocol that can represent all types of seismic loadings. This paper focuses on the effects of various loading histories on the deformation capacity and energy dissipation capacity of the beams. Cyclic loading tests of steel beams were performed. In addition, some experimental results from published tests were also collected to form a database. This database was used to evaluate the energy dissipation capacity of steel beams suffering from ductile fracture under various loading histories. Copyright © 2011 John Wiley & Sons, Ltd.     

A case study of the application of a microcomputer-based favorability evaluation system: Uranium exploration in the Alligator Rivers Uranium Field, Northern Territory, Australia

We have attempted to develop an expert system to evaluate the favorability of uranium exploration projects using the Data-directed Numerical Method (DdM). We developed the system for the Macintosh personal computer, so as to facilitate the iterative processes of trial and error inherent in the DdM. In a detailed case study, we use the above system to evaluate the favorability of the Alligator Rivers Uranium Field, Northern Territory, Australia. The favorability of the target area is evaluated by considering several exploration parameters related to the geological factors controlling ore formation. The selection of appropriate exploration parameters is important to the success of the method. We then selected 15 model cells to validate each exploration parameter and also to design the exploration model by the application of the chi-square test. The result of the favorability evaluation, as displayed by the developed system, indicates that our designed exploration model is acceptable. By employing the above processes we conclude that the system using the DdM is applicable as an exploration tool and is useful in establishing an exploration model.     

Seismic-response-controlled structure with active mass driver system. Part 1: Design

An Active Mass Driver (AMD) system is proposed to suppress actively the response of a building to irregular external excitations such as earthquakes and typhoons. This system has been introduced to an actual ten-storey office building for the first time in the world. The system controls the motions of a structure by means of an external energy supply. It consists of an auxiliary mass installed in a building and an actuator that operates the mass and produces a control force which counters disturbances to the building. The design method of the AMD system, including the location of the installation and the capacity and stability of the system, is proposed. Simplification of the control algorithm is also described.     

Analysis of the geographical accessibility of neurosurgical emergency hospitals in Sapporo city using GIS and AHP

To improve the geographical accessibility of neurosurgical emergency hospitals for elderly people, we developed several alternative site plans for a new neurosurgical emergency hospital in Sapporo, Japan. Hospitals, population data, routes, and the numerical information for the Analytic Hierarchy Process computations were input into a Geographical Information System. Pairwise comparison revealed the following weights which were assigned to each of the four criteria: 0.674 for availability of hospital beds; 0.169 for the maximum road distance of the shortest routes; 0.101 for the elderly population within a 3‐km radius; and 0.056 for the median road distance of the shortest routes. The alternative proposed could cover 4000 more elderly people in the 3‐km radius of the hospitals. The integration of Geographical Information Systems and the Analytic Hierarchy Process constitutes a powerful tool for analysing traffic conditions in mid‐sized cities and for suggesting city planning to improve prognosis of stroke.