对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

北黄海海底麻坑群形态的定量研究及控制因素

本文基于高分辨率多波束水深数据和反向散射强度数据,对北黄海海底麻坑群的形态参数进行定量研究,结合水深、地形坡度和后向散射强度的变化准确界定了麻坑的轮廓,识别出圆形、椭圆形、拉长型麻坑共282个,并在ArcGIS软件中对其形态参数进行了分析计算,麻坑的平均长轴1.36 km,短轴0.78 km,直径0.94 km,面积0.88 km2,平均周长3.82 km,长宽比1.83,深度0.3~2.5 m,平均面积密集度13%,麻坑的剖面形态有麻坑边缘凹陷、中部有明显凸起（W1型）,麻坑边缘凹陷、中部略凸起（W2型）,麻坑中部单纯凹陷（V型）,分别集中分布在麻坑群的北部、南部、西部。麻坑的平面规模大、深度小的原因与地层中形成麻坑的游离气体浓度较小有关,也可能受到了地震、海啸等外力的诱发。麻坑的长轴优势走向为ENE-WSW、NNE-SSW,底流对其形状的塑造起了较大作用,部分麻坑成串排列,形成串珠状的麻坑链,其排列方式受到海底古河道、古潟湖等沉积地层结构的控制。海底麻坑群发育区反向散射强度为-60~-71 dB,麻坑内部较麻坑外部平均高5 dB,可能为麻坑内部气体泄漏引起海底沉积物被剥蚀后残留下的粗颗粒物质或海底生物活动留下的遗迹导致的。     

Laboratory tests of vortex-induced vibrations of a long flexible riser pipe subjected to uniform flow

Laboratory tests have been conducted on vortex-induced vibration (VIV) of a long flexible riser towed horizontally in a wave basin. The riser model has an external diameter of 16 mm and a total length of 28.0 m giving an aspect ratio of about 1750. Reynolds numbers ranged from about 3000 to 10,000. Fiber optic grating strain gages are adopted to measure the dynamic response in both cross-flow and in-line directions. The cross-flow vibrations were observed to vibrate at modes up to 6 and the in-line reached up to 12. The fundamental response frequencies can be predicted by a Strouhal number of about 0.18. Multi-mode responses and the asymmetry of the bare pipe response in uniform flow were observed and analyzed. The experimental results confirmed that the riser pipe vibrated multi-modally despite it being subject to a uniform current profile and all of the excited modes vibrated at the Strouhal frequency. The asymmetrical distribution of displacement mainly resulted from the modal composition. Higher harmonics of the VIV response such as the third, fourth and fifth harmonics frequencies were found to be steady over the entire duration of the test even if they varied along the length of the riser pipe.     

Hydrodynamic characteristics and sediment transport of a tidal river under influence of wading engineering groups

By taking the Yong River for example in this paper, based on the multiple measured data during 1957 to 2009, the change process of runoff, tide feature, tidal wave, tidal influx and sediment transport are analyzed. Then a mathematical model is used to reveal the influence mechanism on hydrodynamic characteristics and sediment transport of the wading engineering groups such as a tide gate, a breakwater, reservoirs, bridges and wharves, which were built in different periods. The results showed the hydrodynamic characteristics and sediment transport of the Yong River changed obviously due to the wading engineering groups. The tide gate induced deformation of the tidal wave, obvious reduction of the tidal influx and weakness of the tidal dynamic, decrease of the sediment yield of flood and ebb tide and channel deposition. The breakwater blocked estuarine entrances, resulting in the change of the tidal current and the reduction of the tidal influx in the estuarine area. The large-scale reservoirs gradually made the decrease of the Yong River runoff. The bridge and wharf groups took up cross-section areas, the cumulative affection of which caused the increase of tidal level in the tidal river.     

西沙群岛生物碳酸盐的成因和沉积:来自活体仙掌藻的证据

A recent island survey reveals that the Xuande Atoll and the Yongle Atoll in the Xisha Islands can be classified into one of two systems: the depleted atoll system and growth atoll system; the survey also indicates that the decreased area of several shoals is an unbearable burden for the Xisha Islands, of which the largest island area is 2.13 km2 and the minimum elevation is 1.4 m. According to a survey on the ecological characteristics of Halimeda in the Laolongtou breaker zone of Shidao Island in the Xisha Islands, the green and white living Halimeda are collected,the isotopic ages of 14 C contained in the Halimeda are shown to be 27 years and 55 years, respectively, and carbonate mainly occurs in five types, i.e., luster, segment, sand, sand grain, and marl in the formation. The Halimeda segments mainly provide the carbonate sediments of long-term biogenic deposits in the reef environment and the annual productivity per area is 60–100 g/m2; the characteristics of the microstructure of the Halimeda are analyzed, the aragonite raphide carbonate is deposited and enriched in the cortexes, medullas and cysts, and the Halimeda generally contain major elements such as C, O, Ca, Cl, Mg, K, Na, S and Al, and are rich in trace elements such as tellurium(Te), rhodium(Rh) and strontium. It is believed that the Halimeda grow slowly,including the biotic community of reef corals in the reef areas, thus they possess an environmental remediation capacity, but it takes much time to remedy the environment, and it is necessary to make the law to protect the diversity and vulnerability of the Xisha marine ecology, the ecology of the reef community and the island environment in a scientific way. As indicated in the survey, under the background of global warming and sea-level rise, the discovery of large amounts of Halimeda in the Laolongtou sea area is significant for the natural increase of the depleted atoll system of the Xuande Atoll, while the Halimeda segments represent the primary form of the fossil Halimeda, of which the species can be identified and preserved in great numbers under geological conditions. The Miocene was discovered in large amounts in the Xichen-1 well, therefore the study on the characteristics and mechanism of Halimeda carbonate sediments plays a pivotal role in the formation and construction of organic reefs in the South China Sea as well as oil and gas exploration.     

Tensile stiffness analysis on ocean dynamic power umbilical

Tensile stiffness of ocean dynamic power umbilical is an important design parameter for functional implementation and structural safety. A column with radial stiffness which is wound by helical steel wires is constructed to predict the tensile stiffness value of umbilicals in the paper. The relationship between the tension and axial deformation is expressed analytically so the radial contraction of the column is achieved in the relationship by use of a simple finite element method. With an agreement between the theoretical prediction and the tension test results, the method is proved to be simple and efficient for the estimation of tensile stiffness of the ocean dynamic power umbilical.     

内核及外围尺度对热带气旋强度影响的数值试验

采用WRF数值模式模拟并对热带气旋尺度与强度关系进行了探讨,且初步诊断分析了内核及外围尺度对热带气旋强度影响的可能机制,结果表明:(1)内核区较大时的缩放引起角速度变化是其影响热带气旋强度的机制之一;(2)内核区较小时的进一步收缩引起的眼区次级环流破坏是其影响热带气旋强度的一种机制;(3)外围尺度变化造成低层上升至高层的水汽总量变化,是其影响热带气旋强度的一种机制;另一方面,外围尺度发生变化使得低层气流向热带气旋内的辐合减少,亦是其影响热带气旋强度的机制之一。     

天然叶蜡石及其热处理产物的电子顺磁共振（EPR）研究

利用EPR研究了18个天然叶蜡石样及其4个热处理产物。发现天然叶蜡石有三个EPR吸收带：A（g有效≈11．4），B（g有效≈4．3）和C（g有效≈2．0），其中A是叶蜡石的特征谱。A在＞400℃热处理时与B在强度上呈消长关系；＜400℃热处理时A和B均随温度升高而加强；900℃时A消失，B的强度远远大于原样中的A；C附有超精细结构谱（g″=2.04,g┴=1.96,A″≈A┴=19.5×10^-4T），C在热处理时呈现复杂的变化。结果表明，A吸收带属于叶蜡石八面体AI（Fe)-O4(OH)2中的Fe^3 ，B吸收带归于八面体AI（Fe)-O2(OH)4中的Fe^3 ，C吸收带是无机自由基（如AI－O^-Si)和有机自由基的叠加谱；900℃热处理后八面体层中配位发生了变化：AI（Fe)-O4(OH2)→AI（Fe)-O5 H2O↑，AI(Fe)-O2(OH)4→AI(Fe)-O4 H2O↑；类质同象置换AI^3 的不仅有Fe^3 ,而且有Fe^2 离子。     

国内外钼资源供需形势分析

全面、系统地建立钼矿石、钼矿粉分析方法,对钼元素研究开发和保障钼矿工业发展具有重要意义。目前多采用酸溶或碱熔样品后进行分析,其不足是测定钼含量的范围窄,消耗样品量大,还需使用大量酸碱,且受仪器限制,分析高含量钼时多需对样品溶液进行数次稀释,使分析步骤更加繁琐。发射光谱法则可避免上述问题,但用之准确分析高含量钼矿石钼矿粉尚有测试方法的瓶颈需要突破。本文研究通过内标元素、分析线对、缓冲剂配比、电流程序等环节的实验分析,建立了固体进样-交流电弧发射光谱法测定钼矿石中高含量钼的分析方法：优化样品与光谱缓冲剂质量比至1∶2,优化分析线对,截取曝光时间35s,采用以国家一级合成硅酸盐光谱分析标准物质和国家一级矿石标准物质组成的自研标准系列制作标准曲线,由全谱交直流电弧发射光谱仪自动扣除分析线和内标线背景后以对数坐标二次曲线拟合计算,使测定范围扩展为500～500800μg/g,检出限为27.38μg/g,相对标准偏差（RSD）为3.28%～8.30%,相对误差为-0.43%～0.73%。结果表明,本文方法在实现绿色分析的同时,在检出限相当、精密度合格的条件下,一次性分析高含量钼的上限从5%提高至50%。

     

南海周边相对海平面变化特征及2004年苏门答腊地震影响分析

利用南海周边1989-2014年的潮汐资料和GPS长期观测资料,分析了南海周边相对海平面变化特征,以及2004年苏门答腊地震对该区域相对海平面变化的影响。研究结果表明,南海周边的相对海平面变化以上升为主,平均上升速率（4.53±0.20） mm/a,高于全球平均速率,且2004年后上升趋势加剧;南海周边相对海平面呈现6类较典型的变化特征,并存在与板块构造相对应的分区聚集现象,形成了中国东南和越南沿海、马来半岛、加里曼丹岛北部、菲律宾群岛等4个变化特征区。受2004年苏门答腊大地震的影响,马来半岛、南沙和西沙海域的地壳形变由上升趋势转为下沉,加剧了相对海平面的上升;中国东南沿海和菲律宾群岛受地震影响较小;越南沿海和加里曼丹岛北部区域的地震影响还有待进一步研究。