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191.
The change in the zonal sea surface temperature gradient (ZSSTG) across the equatorial Pacific plays an important role in the global climate system. However, there has not yet been a consensual conclusion about the changing ZSSTG at either a short-term (from 20 to 90 years) or a long-term time scale (longer than 90 years) in the literature. In this study, the uncertainty of the trend in ZSSTG for different sub-periods since 1881 was examined using four interpolated datasets and four un-interpolated datasets. It was found that the trend in ZSSTG on the short-term time scale could be significantly influenced by internal variability such as the El Niño–Southern Oscillation and the Pacific Decadal Oscillation. On the long-term time scale, the sign of the ZSSTG trend depends on the dataset used. In particular, it was not possible to draw a uniform conclusion about the secular trends in ZSSTG in recent history, given the high sensitivity of the ZSSTG trends to the period, dataset, and regions used to calculate the trends. Our results imply that it may not be possible to detect the response of ZSSTG to global warming until a longer data record becomes available in the future. 相似文献
192.
对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备 总被引:2,自引:0,他引:2
本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础. 相似文献
193.
??LAMBDA????????GNSS????????????????????????????????????????????????????????????????????????Ratio??????????????????Ratio?????????????????????L1??L2?????λ????????????????????÷????????????????????????????????????????????????????????? 相似文献
194.
We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future. 相似文献
195.
唐九安 《大地测量与地球动力学》2000,20(4):64-70
介绍了超导重力仪 GWR- TT70 ( 1986- 0 1~ 1993- 12 )在武昌站的观测数据及潮汐参数的计算结果。采用了非数字滤波调和分析法 ,计算出该仪器观测重力值对气压的响应系数和长周期波潮汐参数 ,得到潮汐因子在 8年中的稳定度为 0 .5%以内。最后分析了仪器漂移特征和武昌站重力长期变化的可能量级。 相似文献
196.
197.
藏、汉对比视角下的城市空间环境认知研究——以中国西藏日喀则市为例 总被引:2,自引:0,他引:2
以分布式认知理论为基础,采用质性研究法、数量分析法以及GIS空间分析技术,探讨日喀则市藏族、汉族居民对城市空间环境认知的异同性。研究表明,藏族对城市空间环境的认知得分高于汉族对城市空间环境的认知得分。藏族、汉族对城市空间环境的认知水平可分为高、中、低3类。认知空间呈现圈层结构与混合结构模式,可分为藏汉一致性、藏族独立以及汉族独立3种认知空间类型。影响居民对城市空间环境的认知因素有共性因素和差异性因素。景观走廊质量、宜居性、基础设施两项是藏族居民关注的主要影响因素,汉族居民更关注公共服务设施的服务能力以及对当地文化的融入感。 相似文献
198.
The community dynamics and potential carbon ?xation of intertidal macroalgae were investigated monthly from April 2014 to April 2015 in the northwest coast of Yellow Sea. Seasonal variations in biomass and carbon ?xation were presented and showed close relationship with community structure.The carbon ?xation rate ranged from 0.48±0.13 mg C/(g FW ·d) to 4.35±0.12 mg C/(g FW ·d). Sargassum thunbergii, Chondrus ocellatus and Ulva intestinalis were three most in?uential species which contributed27%, 21.9% and 18.5% variation of carbon ?xation rate, respectively. Standing carbon stocks ranged from7.52 g C/m~2 to 41.31 g C/m~2, and estimated carbon stocks varied from 11.77 g C/m 2 to 96.49 g C/m~2. The larger dif ference between estimated and standing carbon stocks implied that more ?xed carbon was exported from the community in summer and autumn than in winter. This study suggested that intertidal macroalgal community could provide a potential function in carbon ?xation of coastal ecosystem. 相似文献
199.
Due to the specific dynamics, the probes located at the halo orbits or Lissajous orbits around the Earth-Moon collinear libration point L1 or L2 are always studied in the synodic system to understand their trajectories. In fact, they are also orbiting the Earth in a distant Keplerian ellipse. Because of their intrinsic orbital instability, in the orbit prediction the initial errors propagate more prominently than those of the normal orbiting satellites, this requires special attention in the orbit design, maneuver, and control. Despite of all this, they are similar to the normal orbiting satellites in orbit determination and hardly require other special attentions. In this paper, the quantitative results of error propagation under the unstable dynamics, together with the theoretical analysis are presented. The results of precise orbit determination and short-arc orbit predictions are also shown, and compared with the results from the Beijing Aerospace Control Center. 相似文献
200.
疫情期间全球各地一次排放大幅削减,而臭氧等二次污染的响应则存在着区域间差异.结合地面和卫星观测发现,同在氮氧化物大幅下降的情况下,臭氧在东亚和欧洲呈现出可达14ppb的上升信号,而北美则下降为主(约2-4ppb).我们结合气象分析和臭氧敏感性进一步讨论了臭氧响应差异性的原因,一方面受臭氧与前体物间关系的影响;另一方面来... 相似文献