Insulin-like growth factors (IGFs) are key regulators of development and growth. Here, we characterized the igf2 gene from olive flounder (Paralichthys olivaceus) and determined its temporal and spatial expression. We set up an in-vitro protein expression system in eukaryotic human embryonic kidney (HEK293T) cells and explored its effects on cell proliferation. The flounder igf2 cDNA contained a 648-bp open reading frame (ORF) encoding a protein of 215 amino acids (aa), which spanned the complete signal peptide (47 aa), mature peptide (70 aa), and E domain (98 aa). In adult flounder, igf2 mRNA was detected in all selected tissues. In early development, igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos. In-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region, floor plate, hypochord, otic vesicle, and pectoral fin during embryogenesis. Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells. In addition, the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma (HeLa) and HEK293T cells. These results provide new information about the structural and functional conservation, expression patterns, and biological activity of the igf2 in teleosts.
Acta Geochimica - Mercury (Hg) is a global pollutant and can be accumulated in the food chain, posing exposure risks to humans. In this study, rice plants and corresponding rhizosphere soil samples... 相似文献
Previous studies on iron isotope compositions of subduction zone magmas have revealed significant and complex variations that have great bearings on petrogenetic processes in the mantle wedge, e.g., partial melting, fluid metasomatism and redox state. However, interpretations for the fractionations are highly debatable and lack direct constraints from mantle wedge peridotites. This study presents iron isotope compositions for whole rocks and mineral separates in fresh forearc peridotites from the Yushigou ophiolite, North Qilian orogen in northern Tibet. Major and trace element compositions of whole rock and mineral indicate that the peridotites are highly depleted forearc peridotites overprinted by melt metasomatism, in contrast to the long‐holding opinion that the peridotites are derived from mid‐oceanic ridges. The minerals fall on a line with a slope of ~1 on the plot of δ56Fe vs. δ56Fe, indicating isotope equilibrium between minerals. δ56Fe fractionation between olivine and orthopyroxene is within the range of 0~0.05, while fractionation between olivine and spinel is about 0.05~0.10. The fractionation trend between olivine and spinel is opposite to previous theoretical and experimental constraints, which may be due to substantial Cr substitution into the spinel. This indicates that negative correlations between spinel Cr#, fO2 and spinel δ56Fe in previous studies are probably a reflection of gradual Cr enrichment in spinel during melt extraction, and spinel δ56Fe values are not a proxy for oxygen fugacity. Whole rock δ56Fe values are well correlated with mineral δ56Fe values, varying from overlapping with depleted mantle to slightly lower than depleted mantle. Therefore, variations in iron isotope compositions of subduction zone magmas are probably due to combined effect of source heterogeneity and partial melting fractionation. 相似文献