脊尾白虾(Exopalaemon carinicauda)组织蛋白酶D基因的克隆及其表达分析

采用RACE技术获得了总长为1853bp的脊尾白虾(Exopalaemon carinicauda)组织蛋白酶D基因全长cDNA序列。该基因5′和3′非编码区分别为120bp和572bp,开放阅读框为1161bp,推测编码386个氨基酸,预测分子量为42.36kDa,理论等电点为7.54,命名为EcCatD基因。同源性和系统进化分析表明,EcCatD基因与斑节对虾、美洲螯龙虾的相似性分别为86%和80%,与斑节对虾和美洲螯龙虾紧密聚为一支。荧光定量RT-PCR结果表明,EcCatD基因在血细胞中的相对表达量最高,其次为肝胰腺。该基因在鳗弧菌和WSSV感染后的脊尾白虾血细胞和肝胰腺中的表达量显著增加,并具有不同的时空表达趋势。结果表明EcCatD基因在脊尾白虾免疫反应中具有重要作用。     

Simulation of wind-induced near-inertial oscillations in a mixed layer near the east coast of Korea in the East/Japan Sea

Using a simple damped slab model,it was possible to show that a local wind induced 88%(15 of 17)of the near-inertial oscillations(NIO)observed in the mixed layer near the east coast of Korea from 1999 to 2004.The model,however,overestimated the energy level in about two-thirds of the simulated cases,because the slab model was forced with winds whose characteristic period was shorter than the damping time scale of the model at 1.5 d.At the observation site,due to typhoons and orographic effects,high-frequency wind forcing is quite common,as is the overestimation of the energy level in the slab model results.In short,a simple slab model with a damping time-scale of about 1.5 d would be enough to show that the local wind was the main energy source of the near-inertial energy in this area,but the model could not be used to accurately estimate the amount of the work done by the wind to the mixed layer.     

银鲳(Pampus argenteus)幼鱼饥饿前后侧囊及肝脏超微结构的变化

分别采用扫描和透射电镜, 观察了正常和饥饿5d银鲳(Pampus argenteus)幼鱼的食道侧囊及肝脏超微结构的变化。结果表明, 正常银鲳幼鱼侧囊内壁具有许多平均高度为800?m的乳突, 上有次级突起。乳突黏膜由复层扁平上皮构成, 上皮细胞表面有指纹状的微嵴, 有缓解上皮细胞的机械损伤作用; 银鲳肝脏具肝血窦、胆小管、狄氏窦等结构, 肝细胞排列较紧密, 内含丰富的细胞器和脂滴, 核仁大而明显, 线粒体数目多, 多分布在细胞核、内质网和狄氏窦周围。饥饿后, 侧囊乳突变低矮, 平均高度700?m, 乳突上黏膜变薄, 逐渐退缩, 次级突起顶端露出了尖刺状的角质刺; 肝细胞体积缩小, 核变小, 细胞器数目减少, 线粒体水肿, 粗面内质网数量显著降低, 脂滴数量降低, 这是银鲳幼鱼对饥饿的生理性防御。     

半滑舌鳎(Cynoglossus semilaevis)双链RNA依赖的蛋白激酶R(PKR)基因克隆和实时定量表达分析

采用RACE方法克隆了半滑舌鳎(Cynoglossus semilaevis)PKR基因(CsPKR), 获得了CsPKR全长cDNA序列为2907bp, 其中包含1959bp的开放阅读框, 100bp的5′非编码区和848bp的3′非编码区。保守结构域分析显示推导的CsPKR氨基酸在N端存在两个特异的dsRBD(dsRNA binding domain dsRBD)结构域, 在C端具有多个保守的激酶活性位点, 包括ATP结合位点和底物配体结合位点, 以及活性环结构A-loop。系统进化树分析显示鱼类、两栖类、鸟类及哺乳类的PKR具有共同的进化起源, CsPKR与牙鲆PKR的亲缘关系最为密切。荧光定量PCR结果显示, CsPKR基因在健康鱼多个组织中广泛表达, 在脑中的表达最高, 头肾中表达最低。经鳗弧菌和淋巴囊肿病毒分别感染后, CsPKR基因在免疫相关组织中呈现上调表达趋势, 其中感染鳗弧菌12h后在血液中表达量较对照组上升了9.28倍, 在感染淋巴囊肿病毒24h后, 在肝脏中的表达达到最大, 为对照组的9.97倍。以上结果暗示CsPKR基因在半滑舌鳎响应细菌和病毒免疫应答中起重要作用。     

The vertical structure of the atmospheric boundary layer over the central Arctic Ocean

The tropopause height and the atmospheric boundarylayer （PBL） height as well as the variation of inversion layer above the floating ice surface are presented using GPS （global position system ） radiosonde sounding data and relevant data obtained by Chinas fourth arctic scientific expedition team over the central Arctic Ocean （86°-88°N, 144°-170°W） during the summer of 2010. The tropopause height is from 9.8 to 10.5 km, with a temperature range between -52.2 and -54.10C in the central Arctic Ocean. Two zones of maximum wind （over 12 m/s） are found in the wind profile, namely, low- and upper-level jets, located in the middle troposphere and the tropopause, respectively. The wind direction has a marked variation point in the two jets from the southeast to the southwest. The average PBL height determined by two methods is 341 and 453 m respectively. These two methods can both be used when the inversion layer is very low, but the results vary significantly when the inversion layer is very high. A significant logarithmic relationship exists between the PBL height and the inversion intensity, with a correlation coefficient of 0.66, indicating that the more intense the temperature inversion is, the lower the boundary layer will be. The observation results obviously differ from those of the third arctic expedition zone （800-85° N）. The PBL height and the inversion layer thickness are much lower than those at 870-88° N, but the inversion temperature is more intense, meaning a strong ice- atmosphere interaction in the sea near the North Pole. The PBL structure is related to the weather system and the sea ice concentration, which affects the observation station.     

基于外部形态特征的东南太平洋茎柔鱼种群结构研究

茎柔鱼是大洋性重要经济头足类,分布范围广泛,种群结构复杂。本文根据2008年5月～2009年10月对智利、秘鲁2个海区采集的茎柔鱼生物学样本,利用胴宽、鳍长、触腕长等11个外部形态指标与胴长之比,分析东南太平洋茎柔鱼种群结构组成及其差异,并建立相应的判别函数。研究认为,采集的渔获物样本中,初步可分为大型和小型2个种群。2个海区间大型群和小型群的外部形态特征差异显著,2个海区利用外部形态特征对种群判别正确率均在60%以上。研究认为,研究认为,外部形态特征可作为划分茎柔鱼种群结构的有效方法之一。     

黑石礁海域生物碳、氮稳定同位素组成的研究

稳定同位素技术是研究生态系统中物质循环与能量流动的有效技术。利用稳定同位素技术研究了黑石礁海域浮游植物和6种海洋生物的δ13 C和δ15组成、营养位置、空间分布和月份变化。结果显示:浮游植物的δ15 C远低于其他物种,不同物种的δ13 C和δ15 N存在明显的差异,且有交叉现象,表明他们同化了不同的碳源;物种的δ15 N值空间差异较大而δ13 C值空间差异不明显,且δ15 N值与距离排污口的远近有关系,越靠近排污口δ15 N越大,与人类活动营养物质的输入有关系,可以用δ15 N监测人类活动对海洋生物的影响;孔石莼的δ15 N月平均变化很大,浮游植物δ15 N值基本没有变化,其他物种没有发生很大的变化;孔石莼是适合短期监测人类活动对海洋影响的物种。     

太湖风浪场的计算与比较

首先探讨了浅水风浪数值模型—SWAN模型应用于模拟内陆湖泊风浪生成和传播变形时的特点。该模型存在不能有效地模拟近固壁边界处风浪场的缺点,以能正确地模拟湖区的风浪场和节约计算时间为原则,确定了计算范围。对太湖进行了风场和风浪场的现场观测。分别利用规范公式和SWAN模型两种方法、根据观测和预报的风场计算了湖区的有效波高,并将计算结果和现场观测值进行了详细比较。结果表明基于观测的风场,利用两种方法所计算的太湖风浪场的精度基本相当;在根据观测的风场、利用SWAN模型计算内陆湖泊的风浪场时,需要精心选择恰当的风场;在根据预报的风场预报湖区风浪场时,SWAN模型的精度要高于规范公式的精度。     

单一地层平台插桩地基土破坏模式及插桩深度有限元分析

自升式平台插桩是地基土在桩靴荷载作用下发生连续塑性破坏的过程,当地基极限承载力等于桩靴荷载时插桩完成。经典土力学极限承载力理论对土体潜在滑动面做了假设,无法有效分析土体内部的破坏过程。因而,研究采用有限元极限分析法对单一地层中插桩时地基土渐进破坏过程进行了模拟,并与Skempton、Terzaghi公式计算的极限承载力进行了对比,取得了较为一致的结果,同时对插桩深度进行了预测。     

Wave Height Transformation and Set-up Between a A Submerged Permeable Breakwater and A Seawall

In this study, we investigated wave transformation and wave set-up between a submerged permeable breakwater and a seawall. Modified time-dependent mild-slope equations, which involve parameters of the porous medium, were used to calculate the wave height transformation and the mean water level change around a submerged breakwater. The numerical solution is verified with experimental data. The simulated results show that modulations of the wave profile and wave set-up are clearly observed between the submerged breakwater and the seawall. In contrast to cases without a seawall, the node or pseudo-node of wave height evolution can be found between the submerged breakwater and the seawall. Higher wave set-up occurs if the nodal or pseudo-nodal point appears near the submerged breakwater. We also examined the influence of the porosity and friction factor of the submerged permeable breakwater on wave transformation and set-up.