产新琼四糖的海洋微泡菌属AG1热稳定性β-琼胶酶的过量表达与鉴定

从微泡菌属AG1（Microbulbifer sp. AG1）克隆得到1302 bp大小的琼胶酶基因,该基因编码产物为一个成熟蛋白（413个氨基酸残基）外加一个信号肽（20个残基）。将不含信号肽片段的琼胶酶在E. coli BL21 （DE3）中进行了异源表达和纯化。使用琼脂糖作为底物,该重组琼胶酶的最适反应温度和pH分别为60℃和7.5。该重组酶表现出优良的热稳定性,在50℃和60℃下处理1 h,重组琼胶酶仍能分别保持67%和19%的残余酶活力。除了SDS,重组琼胶酶对于其他测试的抑制剂、去垢剂和尿素变性剂有着较好的抗性。利用薄层色谱和以对硝基苯-α/β-D-吡喃半乳糖苷为底物的酶活力分析结果表明,该重组琼胶酶为β型琼胶酶,它水解琼脂糖的主要终产物为新琼四糖,而且不同聚合度的酶解产物具有抗氧化活性。     

Modeling of the eddy viscosity by breaking waves

Breaking wave induced nearsurface turbulence has important consequences for many physical and biochemical processes including water column and nutrients mixing,heat and gases exchange across air-sea interface.The energy loss from wave breaking and the bubble plume penetration depth are estimated.As a consequence,the vertical distribution of the turbulent kinetic energy(TKE),the TKE dissipation rate and the eddy viscosity induced by wave breaking are also provided.It is indicated that model results are found to be consistent with the observational evidence that most TKE generated by wave breaking is lost within a depth of a few meters near the sea surface.High turbulence level with intensities of eddy viscosity induced by breaking is nearly four orders larger than υwl(=κuwz),the value predicted for the wall layer scaling close to the surface,where uw is the friction velocity in water,κ with 0.4 is the von Kármán constant,and z is the water depth,and the strength of the eddy viscosity depends both on wind speed and sea state,and decays rapidly through the depth.This leads to the conclusion that the breaking wave induced vertical mixing is mainly limited to the near surface layer,well above the classical values expected from the similarity theory.Deeper down,however,the effects of wave breaking on the vertical mixing become less important.     

钱塘江河口盐度数值模拟

强潮河口盐水入侵对饮用水源地危害极大。基于平面二维水动力盐度模型, 对典型强潮河口—钱塘江的水动力及盐水入侵过程进行了数值模拟研究。结果表明枯水径流时盐度变化与潮位过程曲线类似, 潮差对盐度大小影响显著, 径流量的增加将逐渐减小其相似程度。当流量增加到一定程度后, 继续增加的一定径流量所产生的抑咸效果减弱, 水资源有效利用率降低, 此时允许水源地盐度超标并改从蓄淡避咸水库取水可有效节约水资源。盐度平面分布显示, 盐水入侵在强潮河口弯道处受涨潮流主流线影响明显, 靠近主流线一岸的盐度大于对岸, 单从盐水入侵角度考虑, 强潮河口弯道段的取水口应设置在远离涨潮流主流线一岸。钱塘江河口盐度数值模拟对于研究减轻盐水入侵对水源地危害的措施具有指导意义。     

黄东海表层沉积物中磷的赋存形态和分布特征及其对生态系统的潜在影响

For better understanding the phosphorus(P) cycle and its impacts on one of the most important fishing grounds and pressures on the marine ecosystem in the Yellow Sea(YS) and East China Sea(ECS), it is essential to distinguish the contents of different P speciation in sediments and have the knowledge of its distribution and bioavailability. In this study, the modified SEDEX procedure was employed to quantify the different forms of P in sediments. The contents of phosphorus fractions in surface sediments were 0.20–0.89 μmol/g for exchangeable-P(Exch-P), 0.37–2.86 μmol/g for Fe-bound P(Fe-P), 0.61–3.07 μmol/g for authigenic Ca-P(ACa-P), 6.39–13.73μmol/g for detrital-P(DAP) and 0.54–10.06 μmol/g for organic P(OP). The distribution of Exch-P, Fe-P and OP seemed to be similar. The concentrations of Exch-P, Fe-P and OP were slightly higher in the Yellow Sea than that in the East China Sea, and low concentrations could be observed in the middle part of the ECS and southwest off Cheju Island. The distribution of ACa-P was different from those of Exch-P, Fe-P and OP. DAP was the major fraction of sedimentary P in the research region. The sum of Exch-P, Fe-P and OP may be thought to be potentially bioavailable P in the research region. The percentage of bioavailable P in TP ranged from 13% to 61%. Bioavailable P burial flux that appeared regional differences was affected by sedimentation rates, porosity and bioavailable P content, and the distribution of bioavailable P burial flux were almost the same as that of TP burial flux.     

Evaluation ofAntitumor, Immunomodulatory and Free Radical Scavenging Effects of A New Herbal Prescription Seaweed Complex Preparation

Seaweed Complex Preparation （SCP） is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs, and it has been used for treatment of lung cancer, liver cancer and digestive cancer. However, little infor- mation is available about the pharmacodynamic basis. The antitumor, immunomodulatory and free radical scavenging effects of SCP were evaluated in this study. Transplanted tumor in vivo method was used to determine the antitumor effect. The effects on spleno- cyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell （CRBC） method respectively. The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated. It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22, and promote proliferation of splenocytes and phagocytosis of macrophages. SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals. The antiturnor effects of SCP might be achieved by im- proving immune system and scavenging free radicals, which is in accordance with the viewpoint of traditional Chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.     

3维地理信息系统技术综述

文中对3维地理信息系统技术进行综述性介绍,扼要地讨论了国内外有关3维地球空间信息的获取、管理、分析与可视化等技术的进展与动态,并分别探讨了它们的优势和局限。     

一种基于信息网格的空间数据分解算法

针对空间数据库数据海量且分布的特点,为了能从这些空间数据中更加有效的收集信息和发现知识,介绍了基于信息网格的空间数据挖掘计算模型,在分析空间数据划分一般原则和方法的基础上,针对该计算模型给出了一个数据划分的初步算法,并通过模拟实验对算法的时间性能进行了评价与分析。     

基于地表校正和河道烧录方法的河网提取

 针对现有的数字高程模型(DEMs)在自动提取虚拟河网过程中易受洼地、平原地区影响,产生不连续的或平行的错误河网的问题,本文基于Arc Hydro Tools,阐述了地表校正(Agree算法)和河道烧录(Stream burning)方法提取虚拟河网的关键步骤和基本思路,并以环渤海地区为例,分析了有(无)河网辅助信息下,河网提取结果的准确度。研究结果表明,增加辅助信息更有助于提高虚拟河网的精度和准确性,且河道烧录方法在虚拟河网的提取上比Agree算法更有优势。由于环渤海研究区平原面积广大,对提取结果有较大的影响,提取结果的精确度不高。针对这一问题,本文综合河道烧录和地表校正(Agree 算法)的特点,提出一种河网提取的改进思路,并选取滦河流域作为验证区。通过对比,改进后的河网提取结果与中国实际河网分布较为吻合,说明该改进思路是较为合理、可信的。     

山东海阳姜家白垩纪水南组化石的发现及保护

姜家位于山东省烟台海阳市北部。在进行烟台地区古生物化石调查的过程中,发现了姜家这一白垩纪水南组良好的露头剖面,在其页岩中赋存丰富的动植物化石,计有玉门延吉叶肢介、长壳延吉叶肢介、中华狼鳍鱼、松柏纲和银杏纲植物化石及昆虫化石。根据前人化石保护评价方法,结合化石的赋存层位、赋存条件、化石类型及保护现状,计算了海阳姜家质量综合指数,认为其属于重点保护区级别;最后根据当地自然情况及工程影响,圈定了保护区范围,并制定了相应的保护措施。     

基于ROMS模式利用集合最优插值同化沿轨海表面高度异常数据的研究

The ensemble optimal interpolation （EnOI） is applied to the regional ocean modeling system （ROMS） with the ability to assimilate the along-track sea level anomaly （TSLA）. This system is tested with an eddy-resolving system of the South China Sea （SCS）. Background errors are derived from a running seasonal ensemble to account for the seasonal variability within the SCS. A fifth-order localization function with a 250 km localization radius is chosen to reduce the negative effects of sampling errors. The data assimilation system is tested from January 2004 to December 2006. The results show that the root mean square deviation （RMSD） of the sea level anomaly decreased from 10.57 to 6.70 cm, which represents a 36.6% reduction of error. The data assimilation reduces error for temperature within the upper 800 m and for salinity within the upper 200 m, although error degrades slightly at deeper depths. Surface currents are in better agreement with trajectories of surface drifters after data assimilation. The variance of sea level improves significantly in terms of both the amplitude and position of the strong and weak variance regions after assimilating TSLA. Results with AGE error （AGE） perform better than no AGE error （NoAGE） when considering the improvements of the temperature and the salinity. Furthermore, reasons for the extremely strong variability in the northern SCS in high resolution models are investigated. The results demonstrate that the strong variability of sea level in the high resolution model is caused by an extremely strong Kuroshio intrusion. Therefore, it is demonstrated that it is necessary to assimilate the TSLA in order to better simulate the SCS with high resolution models.