Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis

Fructose-1,6-bisphosphatase(FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends(RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5′ untranslated region(UTR) of 92 bp, a 3′?UTR of 69 bp, and an open reading frame(ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.     

湖南娄邵冷城市群结构优化研究

运用分形理论等方法对娄邵冷城市群的结构进行了初步分析,结果表明:娄邵冷城市群等级规模结构尚处于低水平;职能结构仅处于工业化初级向中高级阶段转变的过渡时期,区域产业结构水平不高;湘黔铁路沿线和320、207国道沿线城镇已初步具有城市和经济发展的空间集聚特征,但城镇分布分散,集聚优势微弱,空间网络框架尚不成熟.针对其当前存在的问题,提出了娄邵冷城市群结构优化的基本思路.     

Geochemistry of some major chemical composition in marine sediments of the Haitan Island

INTRODUCTIONTheHaitanIslandislocatedalongthecoastinthemiddlesectionofFujianProvince.ItfacestheTaiwanStraitoneast,bordersontheHaitanStraitonwest,andholdsthestrategicpassageoftheFuqingBay.ThegeographicalPOsitionoftheislandissituatedbetween25'33,--25"40'Nand119o41,--119"52'E.Coveringanareaof251.4km2,itisamongthelargestislandsinFujianProvince.BasedondataofacomprehensiveinvestigationintheseaareaoftheHaitanIslandduringMay1990andJune1993,thegeochemicalcharacteristicsofCo,,,N,P,Fe,Al,T…     

Miscellaneous information 1989 (Addendum)

Miscellaneous information 1989 (Addendum)     

APN技术在地震前兆观测系统中的应用

介绍基于GPRS的APN无线网络通讯技术,并应用于地震前兆观测系统。实际运行结果表明,应用该技术系统可提高数据传输的稳定性、可靠性、安全性和实时性,是地震前兆观测数据传输的有效通讯手段。     

Numerical analysis of the sea (lake) breeze dynamics and initialization of the model

In this paper, analysed are the effects of synoptic wind, earth-rotation inertia and land surface roughness on sea (lake) breeze process, on the basis of the calculated results of a 2-D primitive equation model with turbulent energy closure. The results show that a moderate background wind field acts as a trigger for sea (lake) breeze onset, and presents prominent effect on the breeze intensity, inland advance rate and structure feature. The effects of Coriolis force not only make the breeze veering round, but also damp the development of the breeze component normal to shore.The paper also discussed the dynamic method of wind field initialization based on one-site radiosonde. Three approaches of initialization have been tested. In comparison, it was found that the approach of one-dimensional dynamic initialization with nudging term was preferable to the others.     

莱州三山岛黄金海岸沙滩形成过程及沙滩养护建议

利用2010 2012年两年4季莱州三山岛黄金海岸沙滩综合勘测结果,分析上述沙滩形成过程,并进行了沙滩近期变化特征探讨.结果表明,莱州三山岛黄金海岸沙滩年际及年内季节性变化规律均表现出带状冲刷特征,黄金海岸沙滩及其近海海域表现为普遍冲刷特征,冲刷厚度平均＜1.2 m/a.基于莱州三山岛黄金海岸沙滩近期变化特征,建议尽早开展以抛沙养滩为主,并适当辅以硬工程的沙滩养护方案,注重海滩养护工程前期调查,修复和人工再造三阶段的工作,特别应根据设计方案向沙滩开展大量抛沙、保沙和稳沙工作,使受蚀海滩逐步恢复.     

Breaking Criteria for Regular Waves on Gentle Slopes

Nelson pointed out that the wave breaking criterion(H/d)_b for gentle slopes(i<1/100),inwhich H is the wave height and d is the water depth at the breaking point,is smaller than that for beachslopes of i>1/100),i.e.,the value of(H/d)_b for gentle slopes may be smaller than 0.6.Goda indicatedthat the wave breaking criterion given by himself is a result based on theoretical study,so it should be alsocorrect and can be used for gentle beaches,i.e.,the value of(H/d)_b for gentle slopes may be still largerthan 0.7.By use of high order nonlinear wave theory,this problem is analyzed in this study and the reasonwhy there is a large difference between different studies is explained.Moreover,the bottom energy loss dur-ing wave propagation is considered and the critical slope for the absence of wave breaking is also analyzed.