加州鲈鱼的繁殖习性,早期胚胎发育以及孵化与水温的关系

作者对加州鲈鱼繁殖习性及早期胚胎发育进行了阐述,对受精卵孵化与水温的关系进行了研究。结果表明:加州鲈鱼2龄即达性成熟,3—6月繁殖产卵,水温20℃—24℃时达产卵盛期;雄鱼具有筑巢护卵及护苗等生殖习性;水温17.4℃—19.5℃,平均18.3℃时,受精卵孵化时数为74小时25分钟;在实验温度范围内,孵化时数与水温负相关,而与有效积温正相关。有效积温与平均水温负相关。     

中国东南沿海潮灾与防潮对策研究

中国东南沿海是中风暴潮灾害的最严得岸段，本文针对闽，粤，海南三省台风暴潮灾害概况，防潮现状及其存在问题，提出防潮减灾的对策和见解，以祈提醒人们居安思危，防患未然。     

浙江近海大黄鱼 Pseudosciaena crocea( Richardson )的性成熟特性

鱼类性成熟的开始是鱼类生命周期中转入繁衍后代的一个重要阶段。同一世代的个体开始性成熟的年龄、大小,以及延续期限与速度,是种和种内种群对水域生活条件的适应属性之一。 研究鱼类性成熟过程的特点,不仅在解决鱼类种群数量的预测与控制问题中,对补充过程的了解具有重大的意义,而且也为制订合理的渔业经营管理措施提供必要的生物学依据。 本文主要在于探讨浙江近海大黄鱼种群的性成熟特点及其影响因子;其次,讨论该特点在这一种群数量上的意义;并对捕捞规格(法定许可捕捞的鱼体大小和年龄等生物学指标)问题提出初步意见。 在这项工作中,单才根、滕文法和蓝志满等同志曾协助搜集资料、耳石制片和计算工作。在资料的收集中,得到洞头公社和温州地方水产部同的大力支持;本文承张孝威教授热情指导,井承成庆泰副教授等提出宝贵意见,均此谨表谢忱。     

Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

Experimental investigation on a two-part underwater towed system

An experimental set-up is developed and proved to be effective for laboratory study of an underwater towed system. The experimental technique gives a practical method for monitoring the kinematic and dynamic performance of an underwater towed system in a ship towing tank. Both the theoretical and experimental results in the investigation indicate that the hydrodynamic response of a towed vehicle to the wave induced motion of a towing ship can be significantly reduced by applying a two-part tow method. A comparison of the numerical and experimental results in the investigation demonstrates that the numerical simulation results are close to the experimental data, overall agreement between experimental and theoretical results is satisfactory. The results qualitatively verify the mathematical model of a two-part underwater towed system proposed by Wu and Chwang [Wu, J., Chwang, A.T., 2000. A hydrodynamic model of a two-part underwater towed system. Ocean Engineering 27 (5), 455–472].     

盐藻和β-胡萝卜素研究述评

养殖盐藻生产β-胡萝卜素是近十几年新兴起的研究领域，因其应用前途广内，现已成为微藻生物学的研究前沿和热点，预计本世纪末和下世纪初将有更大发展。鉴于我国在这方面文章不系统、不全面，本文从盐藻生物学、养殖学、β-胡萝卜素异构体、β-胡萝卜素形成生理等角度全面概述了该领域的研究现状，并提出存在的问题与研究方向。     

Outer wave number spectrum and statistical distribution of apparent wave number vector

Based on the ray theory and Longuet-Higgins’s linear model of sea waves, the joint distribution of wave envelope and apparent wave number vector is established. From the joint distribution, we define a new concept, namely the outer wave number spectrum, to describe the outer characteristics of ocean waves. The analytical form of the outer wave number spectrum, the probability distributions of the apparent wave number vector and its components are then derived. The outer wave number spectrum is compared with the inner wave number spectrum for the average status of wind-wave development corresponding to a peakness factor P = 3. Discussions on the similarity and difference between the outer wave number spectrum and inner one are also presented in the paper.     

Simple determination of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) using solid-phase microextraction and gas chromatography-mass spectrometry

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r ² > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.     

几种红藻和蓝藻的光合作用色素

分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。     

The effects of sediment from an industrial outfall area on a marine planktonic ecosystem

-Sediment from Xiamen industrial area was added to marine ecosystem enclosures to test the effects on the planktonic communities in Xiamen Bay, China, in April, 1985. Sediments were added at two concentrations, 11.2 and 112 ppm dry wt. respectively. The species of phytoplankton did not change as compared with the control, but the number of diatoms markedly decreased in the sediment treated enclosures. A suppression of photosynthesis was presumed to be due to light attenuation by the added sediment. Microflagellates, zooplankton and bacteria were resistant to sediment increase in water column.