一种用磷酸钙法高效转染HEK 293T细胞方法的建立

利用磷酸钙转染法将lacZ-pShuttle质粒导入HEK 293T细胞中,实验证明,pH值、DNA纯度、转染后培养时间和甘油休克时间是影响转染效率的重要因素.转染后培养6h进行甘油休克效果最佳,比培养0h直接进行休克转染效率升高584.4%;甘油休克时间为4.5min时转染效果最佳,比不用甘油休克转染效率升高130.8%.通过对影响转染的几个条件进行优化,建立了1种可以高效转染HEK 293T细胞的方法,且简便易行.     

海洋岛海域水温异常波动与养殖栉孔扇贝死亡的关系

本文对海洋岛海域水温异常波动,与该海域养殖栉孔扇贝大批死亡的关系,进行了研究。首先,总结了几年来海洋岛海域养殖栉孔扇贝死亡的特征。其次,结合水温观测数据分析了水温异常波动与栉孔扇贝大批死亡的关系。得出的结论是:大幅度、跳跃型水温异常波动是导致扇贝死亡主要原因。最后,通过生物试验证实了上述观点。     

南疆博斯腾湖近12.OkaB.P.以来古气候与古水文状况的变化

对南疆博斯腾湖湖相沉积物多手段的综合研究，揭示了近12.0kaB.P.以来古气候与古水文状况的变化过程与特征。结果表明，其气候的演化特征具有明显的西风型环境演变特征。研究还发现气候于12.0ka、10.0ka、4.0ka和1.5kaB.P.存在显著的阶梯式增暖变干特征，其中暖峰主要出现于11.5—11.0ka、9.4ka、7.5—7.0ka、6.5—6.0ka、3.0ka和2.0kaB.P.，气候特征以干暖为主，湖泊水文特征总体表现为盐度增加、水位下降、湖面缩小；而冷峰主要出现于11.0—10.0ka、8.8ka、5.0—4.5ka、3.3ka和1.5kaB.P.，此时湖泊膨胀淡化，湖面上升。在7.0—5.0kaB.P.期间出现的相对暖湿环境特征可能是全新世大暖期最盛期暖湿气候特征的反映，可能与此时西南夏季风势力增强有关，湖泊水文状况明显变好。     

副渔获物对渔业资源的影响及其减少方法的探讨

由于渔具不同的选择性和多鱼种鱼类共栖 ,人们在对目标种类进行捕捞时 ,不可避免地产生副渔获物及其抛弃。由于渔业资源的下降和人们对海洋环境保护意识的加强 ,副渔获物及其抛弃问题日益成为人们关注的焦点。本文对世界范围内的渔业活动所产生的副渔获物进行分析 ,根据副渔获物的种类、被抛弃情况和原因以及对渔业资源的影响 ,提出了减少副渔获物和抛弃的方法。     

Hydrodynamic Forces on Smooth Inclined Cyh''''nders in Oscillatory Flow

-The hydrodynamic forces on a smooth inclined circular cylinder exposed to oscillating flow were experimentally investigated at Reynolds number (Re) in the range 40000-200000 and Keulegan-Capenter number (KC) in the interval from 5-40. In the test, Re number and KC number were varied systematically. The inertia force coefficient (Cu) and the drag force coefficient (CD) in Morison equation were determined from the measured loads and the water particle kinematics. In this analysis a modified form of Morison equation was used since it uses the normal velocity and acceleration. Thus, the applicability of the Cross Flow Principle was assumed. This principle, simply stated, is as follows: the force acting in the direction normal to the axis of a cylinder placed at some oblique angle with the direction of flow is expressed in terms of the normal component of flow only, and the axial component is disregarded. Both the total in-line force coefficient (CF) and transverse force (lift) coefficient (Cf) were analyzed     

渤海南部表层沉积的相特征

通过海上调查及实验室分析,查明渤海南部表层沉积物在结构类型、矿物组合、生物组分及沉积构造等相特征方面具有明显的分区性。渤海南部存在五种沉积相:黄河水下三角洲相、陆架盆地相、海湾相、潮流浅滩相及古滨岸相。黄河水下三角洲相从河口延至水深15—20米处,沉积速率很高,沉积物富含碎屑云母及CaCo_3。陆架盆地相的沉积为粉砂质粘土,具有生物扰动构造。     

Theory and Practice of Soft Clay Engineering

-The shear strength and deformation properties of soft clay are discussed first. Then some methods for predicting the performance of soft clay foundation are proposed. Finally, case histories are presented to illustrate some discussed aspects of soft clay.     

Improved form of wind wave frequency spectrum

The lower frequency part of the theoretical wind wave spectrum proposed by the authors (Wen et al. , 1988a, b,c) has been improved and the form of spectrum is appreciably simplified. In addition to the field data collected in the Bohai Sea region and used in the previous papers, those obtained in the Huanghai Sea, the East China Sea and the South China Sea have been employed so that the improved spectra can be verified on a more extensive observational basis. Computed results agree with the observations well. Further comparisons have been made between the proposed spectra and the JONSWAP spectrum. Though the two types of spectrum are close to each other in form, the former shows, as a whole, better agreement with the observation than the latter. By introducing an improved relation between the peak-ness factor and significant wave steepness, the spectrum contains only significant wave height and period as parameters. For spectra given in this form, the computed peak frequencies coincide approximately wit     

涠洲岛珊瑚礁生态系中浮游动植物与环境因子关系的初步探讨

本文根据1990年4月、10月间的理化分析数据，结合2002年9月涠洲岛珊瑚礁生态环境的调查状况，首次从珊瑚礁生态系的角度初步探讨该岛区浮游动植物与环境因子的关系。结果表明：该岛区浮游动植物的分布具有明显的区域性和季节性，既体现了珊瑚礁生态系具有较高的初级生产力特征，也体现了浮游动植物具有显著的季节交替现象。相关分析显示，水温、盐度、N，P，Si营养盐对浮游植物的种类组成及数量变动均有明显影响，但对浮游动物的影响不大；N是该生态系中浮游植物的限制因子，P限制状况只有在珊瑚礁生长带较深的海域出现，Si呈相对富足状态。