Encystment and excystment of <Emphasis Type="Italic">Gyrodinium instriatum</Emphasis> Freudenthal et Lee

In the present study, we have investigated the conditions influencing encystment and excystment in the dinoflagellate Gyrodinium instriatum under laboratory conditions. We incubated G. instriatum in modified whole SWM-3 culture medium and in versions of modified SWM-3 from which NO₃ ⁻, PO₄ ³⁻, NO₃ ⁻ + PO₄ ³⁻, or Si had been omitted and observed encystment. Percentage encystment was high in the media without N and without P, while the percentage encystment in the medium lacking both N and P was highest. Moreover, to investigate N or P concentration which induced the encystment, Gyrodinium instriatum was also incubated in media with different concentrations of inorganic N and P; the concentrations of NO₂ ⁻ + NO₃ ⁻ and PO₄ ³⁻ were measured over time. The precursors of cysts appeared within 2 or 3 days of a decrease in NO₂ ⁻ + NO₃ ⁻ or PO₄ ³⁻ concentration to values lower than 1 μM or 0.2 μM, respectively. When cysts produced in the laboratory were incubated, we observed excystment after 8–37 days, without a mandatory period of darkness or low temperature. We incubated cysts collected from nature at different temperatures or in the dark or light and observed excystments. Natural cysts excysted at temperatures from 10 to 30°C, in both light and dark, but excystment was delayed at low temperatures. These studies indicate that G. instriatum encysts in low N or P concentration and excysts over a wide temperature range, regardless of light conditions, after short dormancy periods.     

Reynolds-Number Dependence of Gas Dispersion Over a Wavy Wall

Direct numerical simulations of an Ekman layer are performed to study flow evolution during the response of an initially neutral boundary layer to stable stratification. The Obukhov length, L, is varied among cases by imposing a range of stable buoyancy fluxes at the surface to mimic ground cooling. The imposition of constant surface buoyancy flux , i.e. constant-flux stability, leads to a buoyancy difference between the ground and background that tends to increase with time, unlike the constant-temperature stability case where a constant surface temperature is imposed. The initial collapse of turbulence in the surface layer owing to surface cooling that occurs over a time scale proportional to \(L/u_*\), where \(u_*\) is the friction velocity, is followed by turbulence recovery. The flow accelerates, and a “low-level jet” (LLJ) with inertial oscillations forms during the turbulence collapse. Turbulence statistics and budgets are examined to understand the recovery of turbulence. Vertical turbulence exchange, primarily by pressure transport, is found to initiate fluctuations in the surface layer and there is rebirth of turbulence through enhanced turbulence production as the LLJ shear increases. The turbulence recovery is not monotonic and exhibits temporal intermittency with several collapse/rebirth episodes. The boundary layer adjusts to an increase in the surface buoyancy flux by increased super-geostrophic velocity and surface stress such that the Obukhov length becomes similar among the cases and sufficiently large to allow fluctuations with sustained momentum and heat fluxes. The eventual state of fluctuations, achieved after about two inertial periods (\(ft \approx 4\pi \)), corresponds to global intermittency with turbulent patches in an otherwise quiescent background. Our simplified configuration is sufficient to identify turbulence collapse and rebirth, global and temporal intermittency, as well as formation of low-level jets, as in observations of the stratified atmospheric boundary layer.     

利用ASTER数据估算2002年4月阿克苏地表特征和植被参数

利用ASTER卫星可见光和短波红外波段数据,估算了2002年4月12日阿克苏地区地表特征参数(地表温度T、地表反射率α)和植被参数(归一化植被指数NDVI、修正的土壤调整植被指数MSA-VI、植被覆盖度Pv和叶面积指数LAI等)。结果表明,各种植被参数在沙漠地区较小,而在绿洲中的值较大。同时本文认为ASTER遥感数据有较高的地面分辨率,利用它可以更好地反映地表的植被参数及地表特征参数状况;还指出了ASTER卫星数据在沙漠绿洲中的适用性。     

Effect of electron donors on the fractionation of sulfur isotopes by a marine Desulfovibrio sp.

Sulfur isotope effects produced by microbial dissimilatory sulfate reduction are used to reconstruct the coupled cycling of carbon and sulfur through geologic time, to constrain the evolution of sulfur-based metabolisms, and to track the oxygenation of Earth’s surface. In this study, we investigate how the coupling of carbon and sulfur metabolisms in batch and continuous cultures of a recently isolated marine sulfate reducing bacterium DMSS-1, a Desulfovibrio sp., influences the fractionation of sulfur isotopes.DMSS-1 grown in batch culture on seven different electron donors (ethanol, glycerol, fructose, glucose, lactate, malate and pyruvate) fractionates ³⁴S/³²S ratio from 6‰ to 44‰, demonstrating that the fractionations by an actively growing culture of a single incomplete oxidizing sulfate reducing microbe can span almost the entire range of previously reported values in defined cultures. The magnitude of isotope effect correlates well with cell specific sulfate reduction rates (from 0.7 to 26.1 fmol/cell/day). DMSS-1 grown on lactate in continuous culture produces a larger isotope effect (21-37‰) than the lactate-grown batch culture (6‰), indicating that the isotope effect also depends on the supply rate of the electron donor and microbial growth rate. The largest isotope effect in continuous culture is accompanied by measurable changes in cell length and cellular yield that suggest starvation. The use of multiple sulfur isotopes in the model of metabolic fluxes of sulfur shows that the loss of sulfate from the cell and the intracellular reoxidation of reduced sulfur species contribute to the increase in isotope effects in a correlated manner. Isotope fractionations produced during sulfate reduction in the pure culture of DMSS-1 expand the previously reported range of triple sulfur isotope effects (³²S, ³³S, and ³⁴S) by marine sulfate reducing bacteria, implying that microbial sulfur disproportionation may have a smaller ³³S isotopic fingerprint than previously thought.     

Design and adoption of household tornado shelters for Bangladesh

Death tolls from tornadoes in Bangladesh are the highest in the world due to lack of storm warnings, poor communication, weak housing, and lack of shelters from strong winds in tornadoes and nor’westers. Based on surveys of housing types and designs in the Tangail district, a household tornado shelter is proposed to be placed in the elevated storage platform that is common in houses. The shelter is 2 m tall, 1.2 m wide, and 2–4 m long (4.8–9.6 m³ in volume) with the floor of the shelter placed one meter below the floor of the house. Walls are 7–10 cm thick and made of concrete or an earthen wall stabilized with cement or strengthened with bamboo or bricks. A survey of 200 residents of the region found nearly universal acceptance for the shelter design, and residents were eager for installation of the household shelters. The shelter cost is 2,500–10,000 taka (US50 to50 to 200) depending on local material and labor costs but residents were willing to pay an average of only 1,071 taka (US$21) toward the cost of the shelter. Families with greater income and land holdings and families in villages with recent tornado experience were willing to spend more for a shelter. A pilot project to install household tornado shelters in selected villages and monitor their use, along with continued efforts to issue storm warnings, communicate the warnings, and improve education about storm hazards, will prevent injuries and save lives in Bangladesh and reduce the descent into poverty that results from losses in severe local storms.     

Open boundary value problems in ocean dynamics by finite elements

This paper presents recent results of application of the finite element models to wave overtopping and wave run-up problems in ocean dynamics. Open boundaries are prescribed as natural boundary condition obtained from the continuity equation of the Galerkin finite element formulation. The numerical results are, in general, reasonably good agreements with the histrical field data.     

Tornado shelter-seeking behavior and tornado shelter options among mobile home residents in the United States

Residents of 401 mobile homes in Georgia, Mississippi, Illinois, and Oklahoma were surveyed after they heard a tornado warning. Most residents (69%) did not seek shelter during the warning. Half of those who sought shelter went to the frame house of a friend, neighbor, or relative, and 25% of those sought shelter in a basement or underground shelter. Some of the places where residents sought shelter were of dubious quality, such as their own mobile home, another mobile home, or in an out-building. Twenty-one percent of mobile home residents believed that they had a basement or underground shelter available as shelter during a tornado warning, and about half of those said they would drive to the shelter. Residents said they would drive if the shelter was more than 200 m away. Fifteen percent actually had a basement or underground shelter suitable as shelter within 200 m of their mobile home, but only 43% of the residents would use those shelters. The most common reason cited for not using the shelters was that they did not know the people who lived there. Likewise, a frame house or other sturdy building was within 200 m of 58% of the mobile homes, but only 35% of the residents stated they would use those houses for shelter. Thirty-one percent of mobile home residents had a ditch that was at least 0.5 m deep within 200 m of the mobile home. However, 44% of these ditches had utility lines overhead, 23% had water in them, and 20% had trees overhead. The limited tornado shelter options among mobile home residents in the United States needs to be incorporated into safety instructions so that residents without nearby shelter are allowed to drive to safer shelter.     

Observation of Mercury's sodium exosphere during the transit on November 9, 2006

A rare, but normal, astronomical event occurred on November 9th 2006 (JST) as Mercury passed in front of the Sun from the perspective of the Earth. The abundance of the sodium vapor above the planet limb was observed by detecting an excess absorption in the solar sodium line D₁ during this event. The observation was performed with a 10-m spectrograph of Czerny-Turnar system at Domeless Solar Tower Telescope at the Hida Observatory in Japan. The excess absorption was red-shifted by 10 pm relative to the solar line, and was measured at the dawnside (eastside) and duskside (westside) of Mercury. Between the dawn and dusksides, an asymmetry of total sodium abundance was clearly identified. At the dawnside, the total sodium column density was 6.1×10¹⁰ Na atoms/cm², while it was 4.1×10¹⁰ Na atoms/cm² at the duskside. The investigation of dawn-dusk asymmetry of the sodium exosphere of Mercury is a clue to understand the release mechanism of sodium from the surface rock. Our result suggests that a thermal desorption is a main source process for sodium vapor in the vicinity of Mercury.     

Antifouling herbicides in the coastal waters of western Japan

Residue analyses of some antifouling herbicides (Diuron, Irgarol 1051 and the latter's degradation product M1, which is also known as GS26575), were conducted in waters collected along the coast of western Japan. In total, 142 water samples were collected from fishery harbours (99 sites), marinas (27 sites), and small ports (16 sites) around the Seto Inland Sea, the Kii Peninsula, and Lake Biwa, in August 1999. A urea-based herbicide, Diuron, was positively identified for the first time in Japanese aquatic environments. Diuron was detected in 121 samples (86%) up to a highest concentration of 3.05 microg/l, and was found in 86% of samples from fishery harbours, 89% from marinas, and 75% from ports. Four freshwater samples out of 11 collected at Lake Biwa contained Diuron. Neither Irgarol 1051 nor M1 was found in the lake waters, but both were found in many coastal waters. Irgarol 1051 was found in 84 samples (60%) at a highest concentration of 0.262 microg/l. The concentrations detected were of similar magnitude to those in our previous surveys, taken in 1997 and 1998. M1 was found in 40 samples (28%) up to a highest concentration of 0.080 microg/l. The concentrations detected were generally lower than those found in our previous surveys. The detection frequency among fishery harbours, marinas, and ports was 57-70% for Irgarol 1051 and 25-30% for M1. Ninety-five per cent of the coastal waters in which M1 was detected also contained Irgarol 1051, and 93% of the samples in which Irgarol 1051 was detected also contained Diuron. These results clearly suggest that commercial ship-bottom paints containing both Diuron and Irgarol 1051 are used extensively in the survey area.