In aquatic environments extracellular enzymes are bound to microbial cells or exist in a free and adsorbed state. Various
filters have been used to fractionate these enzymatic activities, but enzymes may be readily adsorbed onto some materials,
and such adsorption can induce errors in the estimation of enzymatic activity. In this study we examined three filters to
determine the most suitable filter for fractionation when estimating proteolytic enzyme activity in seawater. We found that
the polycarbonate Nuclepore membrane, widely used for size fractionation because of its pore-size accuracy, was the most favorable
for this purpose, even though it adsorbed slightly more enzymes than the low-protein-binding polyethersulfone membrane. We
also found that trypsin-and chymotrypsin-type enzymes were more easily adsorbed than aminopeptidases. 相似文献
Lower Cretaceous lacustrine oil shales are widely distributed in southeastern Mongolia. Due to the high organic carbon content of oil shale, many geochemical studies and petroleum exploration have been conducted. Although most of the oil shales are considered to be Early Cretaceous in age, a recent study reveals that some were deposited in the Middle Jurassic. The present study aims at establishing depositional ages and characteristics of the Jurassic and Cretaceous lacustrine deposits in Mongolia. The Lower Cretaceous Shinekhudag Formation is about 250 m thick and composed of alternating beds of shale and dolomite. The Middle Jurassic Eedemt Formation is about 150 m thick and composed of alternating beds of shale, dolomitic marl, and siltstone. The alternations of shale and dolomite in both formations were formed by lake level changes, reflecting precipitation changes. Shales were deposited in the center of a deep lake during highstand, while dolomites were formed by primary precipitation during lowstand. Based on the radiometric age dating, the Shinekhudag Formation was deposited between 123.8 ±2.0 Ma and 118.5 ±0.9 Ma of the early Aptian. The Eedemt Formation was deposited at around 165–158 Ma of Callovian–Oxfordian. The calculated sedimentation rate of the Shinekhudag Formation is between 4.7 ±2.6 cm/ky and 10.0 ±7.6 cm/ky. Shales in the Shinekhudag Formation show micrometer‐scale lamination, consisting of algal organic matter and detrital clay mineral couplets. Given the average thickness of micro‐laminae and calculated sedimentation rate, the micro‐lamination is most likely of varve origin. Both Middle–Upper Jurassic and Lower Cretaceous lacustrine oil shales were deposited in intracontinental basins in the paleo‐Asian continent. Tectonic processes and basin evolution basically controlled the deposition of these oil shales. In addition, enhanced precipitation under humid climate during the early Aptian and the Callovian–Oxfordian was another key factor inducing the widespread oil shale deposition in Mongolia. 相似文献
Diffusion coefficients of Cr and Al in chromite spinel have been determined at pressures ranging from 3 to 7 GPa and temperatures
ranging from 1,400 to 1,700°C by using the diffusion couple of natural single crystals of MgAl2O4 spinel and chromite. The interdiffusion coefficient of Cr–Al as a function of Cr# (=Cr/(Cr + Al)) was determined as DCr–Al = D0 exp {−(Q′ + PV*)/RT}, where D0 = exp{(10.3 ± 0.08) × Cr#0.54±0.02} + (1170 ± 31.2) cm2/s, Q′ = 520 ± 81 kJ/mol at 3 GPa, and V* = 1.36 ± 0.25 cm3/mol at 1,600°C, which is applicable up to Cr# = 0.8. The estimation of the self-diffusion coefficients of Cr and Al from
Cr–Al interdiffusion shows that the diffusivity of Cr is more than one order of magnitude smaller than that of Al. These results
are in agreement with patterns of multipolar Cr–Al zoning observed in natural chromite spinel samples deformed by diffusion
creep. 相似文献
To determine for how long a landslide affects sediment discharge, the sediment yields of 15 check-dam basins were compared with the time series of landslide distributions in a mountain basin in the Tanzawa region, central Japan. The distribution of sediment yield was quantitatively estimated from deposition in the sediment pools of check dams. The relationship between the landslide history and sediment discharge in the Nakagawa River basin was examined for an approximately 80-year period. Two major landslide events occurred during this period: the 1923 Kanto Earthquake and the 1972 disaster caused by heavy rainfall. The resulting trend in sediment discharge of the whole basin, estimated using reservoir sedimentation in the Miho Dam at its base, was nearly constant, with high sediment discharge (2897 m3 km− 2 yr− 1) in the intervening quarter-century, despite the recovery of vegetation on landslide areas in this period. Comparisons of the landslide distributions resulting from the two disasters, the sediment yields of check-dam basins, and the sediment discharge of the whole basin indicate that recent sediment discharge contains landslide debris that was originated by the Kanto Earthquake that occurred over 80 years ago. Thus, to understand high sediment discharge, it is essential to investigate not only the current basin condition and recent events, but also the landslide history of the basin for at least the previous 100 years. 相似文献
We examined seven ultramafic xenoliths from 1~3 Ma alkali olivine basalt reefs near the Eurasian continent and one sample of the host alkali basalt to identify the mantle wedge material and to constrain the origin and evolution of mantle beneath SW Japan. Six xenoliths are from Kurose and one xenolith is from Takashima, northern part of the Kyushu islands, SW Japan. The Sr and Nd isotopic ratios vary from 0.70416 to 0.70773 and from 0.51228 to 0.51283, respectively. The Kurose and Takashima xenoliths have higher Sr isotopic ratios and lower Nd isotopic ratios than those of the peridotite xenoliths from the other arc settings such as Simcoe and NE Japan.
The Kurose xenoliths have less radiogenic Os isotopic ratios (187Os/188Os = 0.123–0.129) than the primitive upper mantle (PUM) estimate and limited variation compared to the other arc xenoliths. Their Os isotope compositions are rather similar to the ultramafic xenoliths from NE and east China. In addition, the samples of the Kurose and Takashima xenoliths plot along a mixing line between ultramafic xenoliths from SE and NE China and a slab component in Sr–Nd–Os isotopic space. Our results suggest that fragments of continental lithospheric mantle from the China craton may exist beneath Kurose and Takashima after the Sea of Japan expansion when the Japanese islands were rifted away from the Eurasian continent during Miocene. Later magmatism due to subduction of the Philippine Sea Plate beneath the SW Japan arc around 15 Ma ago may have introduced fluids or melts derived from slab component, interpreted to be oceanic sediments rather than altered oceanic crust, that possibly modified the original composition of the lithospheric mantle sampled by the peridotite xenoliths from Kurose and Takashima. 相似文献
We characterized the community composition of phytoplankton in the western subarctic Pacific from the pre-bloom to the decline phase of the spring bloom with special reference to decreases in the silicic acid concentration in surface waters as an index for diatom bloom development. Furthermore, responses of heterotrophic bacteria and viruses to the spring bloom were also concomitantly investigated. Under pre-bloom conditions when nutrients were abundant but the surface mixed layer depth was relatively deep, chlorophyll (Chl) a concentrations were consistently low and green algae (chlorophytes and prasinophytes), cryptophytes, and diatoms were predominant in the phytoplankton assemblages as estimated by algal pigment signatures. Together with the shallowing of the mixed layer depth and the decrease in silicic acid concentration, diatoms bloomed remarkably in the Oyashio region, though the magnitude of the bloom in the Kuroshio-Oyashio transition (hereafter Transition) region was relatively small. A total of 77 diatom species were identified, with the bloom-forming diatoms mainly consisting of Thalassiosira, Chaetoceros, and Fragilariopsis species. It has become evident that the carotenoid fucoxanthin can serve as a strong indicator of the diatom carbon biomass during the spring diatom bloom. Differences in the species richness of diatoms among stations generally enabled us to separate the Oyashio bloom stations from the Transition and the Oyashio pre-bloom stations. Relatively high values of the Shannon-Wiener index for the diatom species were also maintained during the Oyashio bloom, indicating that a wide variety of species then shared dominance. In the decline phase of the Oyashio bloom when surface nutrient concentrations decreased, senescent diatom cells increased, as inferred from the levels of chlorophyllide a. Although the cell density of heterotrophic bacteria changed little with the development of the diatom bloom, viral abundance increased toward the end of the bloom, suggesting an increased likelihood of mortality among organisms including diatoms resulting from viral infection. This is the first report on the microbial trophodynamics, including viruses, during the spring diatom bloom in the western subarctic Pacific. 相似文献
Concentrations of Re and Os, and the isotopic composition of Os have been measured in the Japan Sea sediments to assess the response of the Japan Sea to glacial–interglacial climate change and associated weathering fluxes. The osmium concentrations in the sediment samples analyzed vary from 59 to 371 pg/g, and 187Os/188Os from 0.935 to 1.042. Only 187Os/188Os of sediment samples from dark laminations deposited under suboxic to anoxic conditions and having elevated concentrations of Re and Os, and with ≥ 80% hydrogenous Os are explained in terms of seawater composition. Lower 187Os/188Os were observed for sediments deposited during the last glacial maximum (LGM) when planktonic foraminifera from the Japan Sea recorded lighter oxygen isotopic composition. Decrease in dissolved Os fluxes from continents and/or change in the composition of the dissolved load to the Japan Sea are suggested as the driving mechanisms for the observed lower LGM 187Os/188Os. The results of this study, coupled with lower 187Os/188Os during the last glacial observed at other sites from ocean basins with different lithology and contrasting sediment accumulation rates, suggest that this trend is characteristic of the global oceans.
Data from this study show that the Japan Sea recorded higher 187Os/188Os during the current interglacial coinciding with excursions of oxygen isotopic compositions of planktonic foraminifera to heavier values. This is explained in terms of preferential release of 187Os during deglacial weathering and/or higher continental Os flux driven by warm and wet climate. This study demonstrates that Os isotopic composition of reducing margin sediments has immense potential to track variations in the seawater composition. In addition, 187Os/188Os of reducing sediments may be used to draw inferences about local paleoceanographic processes in semi-enclosed basins such as the Japan Sea. 相似文献
This article focuses on the dynamics of using numbers to construct an image of social reality in disaster areas. Numbers are
neither objective nor value-neutral but are rather generated, transmitted and shared with social signification. In other words,
numbers can be thought of as simply socially constructed information. Statistics and other numbers usually work in positive
ways. However, it is also possible that using numbers in the media can lead to unintended messages that could produce negative
consequences. We conducted field studies in disaster-stricken areas of the 2008 Wenchuan earthquake in China and compared
findings to the case of the 1995 Kobe earthquake in Japan in order to examine how numbers—in terms of the amount of donations,
the timeline of reconstruction projects and casualty figures—construct social reality and cause a variety of social dysfunctions. 相似文献
In this study, we focused on the difference in appearances of the convectively coupled equatorial waves (CCEWs) in a simulation with the CCSR/NIES/FRCGC AGCM, between two experiments, one with and the other without implementation of the convective suppression scheme (CSS) in the prognostic Arakawa–Schubert cumulus parameterization. Realistic CCEW modes, i.e., Kelvin, Rossby, mixed Rossby-gravity (MRG), and n = 0 eastward inertio-gravity (EIG) wave modes, were reproduced in the with-CSS experiment, while only Rossby-wave-like signals appeared in the without-CSS experiment.By comparing the structures of the Kelvin wave mode and the Rossby wave mode in two runs, it was suggested that the structural difference between these two modes in conjunction with the difference in the controlling factor of cumulus convection determines the CCEW features. The CSS implemented here is such that cumulus convection is suppressed until the cloud-layer-averaged relative humidity exceeds the threshold of 80%. In the without-CSS model, only Rossby wave modes are coupled with the convection. This is because CAPE controls cumulus convection in this model, and the larger frictional convergence of Rossby wave mode prepares CAPE to generate favorable condition for cumulus convection. In the case of the with-CSS model, on the other hand, cumulus convection is largely controlled by the humidity in the free atmosphere. The convergence associated with the equatorial waves can produce the moisture anomaly to overcome the relative humidity threshold, and maintains the favorable condition for cumulus convection once it starts. In this case, not only Rossby waves but also Kelvin, MRG, and n = 0 EIG waves are reproduced more realistically. It is suggested that inclusion of some kind of mechanism connecting the free tropospheric moisture with the convection under the condition of abundant convective available potential energy could be a key factor for realistic coupling between large-scale atmospheric waves and convection. 相似文献
New radiolarian ages show that the island arc-related Acoje block of the Zambales Ophiolite Complex is possibly of Late Jurassic to Early Cretaceous age.Radiometric dating of its plutonic and volcanichypabyssal rocks yielded middle Eocene ages.On the other hand,the paleontological dating of the sedimentary carapace of the transitional mid-ocean ridge-island arc affiliated Coto block of the ophiolite complex,together with isotopic age datings of its dikes and mafic cumulate rocks,also yielded Eocene ages.This offers the possibility that the Zambales Ophiolite Complex could have:(1)evolved from a Mesozoic arc(Acoje block)that split to form a Cenozoic back-arc basin(Coto block),(2)through faulting,structurally juxtaposed a Mesozoic oceanic crust with a younger Cenozoic lithospheric fragment or(3)through the interplay of slab rollback,slab break-off and,at a later time,collision with a microcontinent fragment,caused the formation of an island arc-related ophiolite block(Acoje)that migrated trench-ward resulting into the generation of a back-arc basin(Coto block)with a limited subduction signature.This Meso-Cenozoic ophiolite complex is compared with the other oceanic lithosphere fragments along the western seaboard of the Philippines in the context of their evolution in terms of their recognized environments of generation. 相似文献