Occurrence of two distinct types of tissue inhibitors of metallo-proteinases-2 in Fugu rubripes

In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fish Fugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAs are composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteine residues, which might form six disulfide bonds as in other animals’ TIMP-2s. Reverse-transcribed polymerase chain reaction analysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expression patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.     

Numerical analysis of equatorial enhancement of geomagnetic sudden commencement

Equatorial behaviour of a polar-originating ionospheric current is examined by solving numerically the continuity equation on a two-dimensional spherical shell with appropriate assumptions for the ionospheric conductivity and the field-aligned source currents. The results show a clear daytime equatorial enhancement of the ionospheric currents in spite of much reduced electric field due to shielding effects of the enhanced Cowling conductivity there. The results are used for interpretation of the preliminary impulse of the geomagnetic sudden commencement.     

Global structure of geomagnetic sudden commencements

The geomagnetic sudden commencements (SSC's) are analyzed by the use of rapid-run magnetograms from eight American zone stations (Koror, Guam, Honolulu, Tucson, Fredericksburg, Sitka, College and Pt. Barrow). The occurrence rate of the equatorial SC¹ reaches the maximum value of 60–70% in the early afternoon. The type (SC¹ or pure SC without a preliminary reverse impulse) of an SSC at the dayside equator coincides well with that in the afternoon side high latitudes. When a pure SC is observed at Koror in the early afternoon, the other seven stations also observe pure SC's, and their onset is almost simultaneous at all the stations. The dayside equatorial SC¹ usually corresponds to SC¹'s in afternoon side high latitudes and pure SC's in middle latitudes (Honolulu and/or Tucson). In this case the onset of the pure SC's in middle latitudes is delayed by several tens of seconds from the SC¹'s which appear almost simultaneously in both the dayside equatorial and the afternoon side high latitude region. In rare cases where SC¹'s are observed at all the stations including Honolulu, their onset time is almost simultaneous. The results are interpreted as showing the polar origin of the equatorial preliminary reverse impulse and the existence of two types of the interaction between the magnetosphere and the shock or discontinuity in the solar wind during SSC. Some discussions are given for the global distribution of the onset time and the waveform of SSC, the equatorial enhancement of the preliminary reverse impulse and the main impulse, and a physical decomposition of the SSC disturbance field.     

Timescale of material circulation in subduction zone: U–Pb zircon and K–Ar phengite double‐dating of the Sanbagawa metamorphic complex in the Ikeda district,central Shikoku,southwest Japan

We have estimated the timescale of material circulation in the Sanbagawa subduction zone based on U–Pb zircon and K–Ar phengite dating in the Ikeda district, central Shikoku. The Minawa and Koboke units are major constituents of the high‐P Sanbagawa metamorphic complex in Shikoku, southwest Japan. For the Minawa unit, ages of 92–81 Ma for the trench‐fill sediments, are indicated, whereas the age of ductile deformation and metamorphism of garnet and chlorite zones are 74–72 Ma and 65 Ma, respectively. Our results and occurrence of c. 150 Ma Besshi‐type deposits formed at mid‐ocean ridge suggest that the 60‐Myr‐old Izanagi Plate was subducted beneath the Eurasian Plate at c. 90 Ma, and this observation is consistent with recent plate reconstructions. For the Koboke unit, the depositional ages of the trench‐fill sediments and the dates for the termination of ductile deformation and metamorphism are estimated at c. 76–74 and 64–62 Ma, respectively. In the Ikeda district, the depositional ages generally become younger towards lower structural levels in the Sanbagawa metamorphic complex. Our results of U–Pb and K–Ar dating show that the circulation of material from the deposition of the Minawa and Koboke units at the trench through an active high‐P metamorphic domain to the final exhumation from the domain occurred continuously throughout c. 30 Myr (from c. 90 to 60 Ma).     

Spatial distribution of the apatite fission‐track ages in the Toki granite,central Japan: Exhumation rate of a Cretaceous pluton emplaced in the East Asian continental margin

The Cretaceous Toki granitic pluton of the Tono district, central Japan was emplaced in the East Asian continental margin at about 70 Ma. The Toki granite has apatite fission‐track (AFT) ages ranging from 52.1 ±2.8 Ma to 37.1 ±3.6 Ma (number of measurements, n = 33); this indicates the three‐dimensional thermal evolution during the pluton's low‐temperature history (temperature in the AFT partial annealing zone: 60–120 °C). The majority of the Toki granite has a spatial distribution of older ages in the shallower parts and younger ages in the deeper parts, representing that the shallower regions arrived (were exhumed) at the AFT closure depth earlier than the deeper regions. Such a cooling pattern was predominantly constrained by the exhumation of the Toki granitic pluton and was related to the regional denudation of the Tono district. The age–elevation relationships (AERs) of the Toki granite indicate a fast exhumation rate of about 0.16 ±0.04 mm/year between 50 Ma and 40 Ma. The AFT inverse calculation using HeFTy program gives time‐temperature paths (t–T paths), suggesting that the pluton experienced continuous slow cooling without massive reheating since about 40 Ma until the present day. A combination of the AERs and AFT inverse calculations represents the following exhumation history of the Toki granite: (i) the fast exhumation at a rate of 0.16 ±0.04 mm/year between 50 Ma and 40 Ma; (ii) slow exhumation at less than 0.16 ±0.04 mm/year after 40 Ma; and (iii) exposure at the surface prior to 30–20 Ma. The Tono district, which contains the Toki granite, underwent slow denudation at a rate of less than 0.16 ±0.04 mm/year within the East Asian continental margin before the Japan Sea opening at 25–15 Ma and then within the Southwest Japan Arc after the Japan Sea opening, which is in good agreement with representative denudation rates obtained in low‐relief hill and plain fields.     

Sediment routing analyses based on chronological changes in hillslope and riverbed morphologies

Recent studies of sediment delivery and budgets in the United States indicate that upland erosion rates at a given time may not explain contemporaneous sediment yields from a drainage basin. This suggests temporal discontinuities in sediment delivery associated with hillslope and channel storage processes. Integration of sediment production, storage and transport is essential to understand sediment routing in basins. We analysed each process chronologically using aerial photographs, monitoring data of sediment movement and annual tree-rings, and then compared estimated temporal changes in sediment production from hillslopes, floodplain disturbance areas and sediment transport in river channels. Toeslopes, floodplains and alluvial fans together contained 59 per cent of sediment eroded from uplands over the last 30 years. Monitoring results of riverbed changes showed that the volume of stored sediment on floodplains decreased exponentially with succeeding floods. The age distribution of floodplain deposits reflected the disturbance history of a river channel, and followed an exponential decrease with age. The results of this study may have important implications for sediment control plans for watersheds in steep regions.     

Monitoring system for environmental risk management of a landfill disposal site

A landfill disposal site can be described as “an artificial vessel” and must satisfy the following three demands (1) storage and disposal of waste; (2) protection of the environment; and (3) benefit for nearby residents by taking advantage of consequential effects. The concept of risk was effectively considered to emphasize the importance of monitoring systems in landfill disposal sites, especially to protect the environment. Because a part of the R&D was involved in creating a monitoring system for a landfill disposal site, the present development and future potential of a system which can detect the leakage of watershield sheets, was discussed.     

U–Pb ages of zircons from metamorphic rocks in the upper sequence of the Hidaka Metamorphic Belt,Hokkaido, Japan: Identification of two metamorphic events and implications for regional tectonics

The Hidaka Metamorphic Belt is a well-known example of island-arc crustal section, in which metamorphic grade increases westwards from unmetamorphosed sediment up to granulite facies. It is divided into lower (granulite to amphibolite facies) and upper (amphibolite to greenschist facies) metamorphic sequences. The metamorphic age of the belt was considered to be ~55 Ma, based on Rb – Sr whole-rock isochron ages for granulites and related S-type tonalities. However, zircons from the granulites in the lower sequence yield U – Pb ages of ~21 – 19 Ma, and a preliminary report on zircons from pelitic gneiss in the upper sequence gives a U – Pb age of ~40 Ma. In this paper we provide new zircon U – Pb ages from two pelitic gneisses in the upper sequence to assess the metamorphic age and also the maximum depositional age of the sedimentary protolith. The weighted mean ²⁰⁶Pb/²³⁸U ages from a biotite gneiss in the central area of the belt yield 39.6 ± 0.9 Ma for newly grown metamorphic rims and 53.1 ± 0.9 Ma for the youngest detrital cores. The ages of zircons from a cordierite–biotite gneiss in the southern area are 35.9 ± 0.7 Ma for metamorphic rims and 46.5 ± 2.8 Ma for the youngest detrital cores. These results indicate that metamorphism of the upper sequence took place at ~40 – 36 Ma, and that the sedimentary protolith was deposited after ~53 – 47 Ma. These metamorphic ages are consistent with the reported ages of ~37–36 Ma plutonic rocks in the upper sequence, but contrast with the ~21–19 Ma ages of metamorphic and plutonic rocks in the lower sequence. Therefore, we conclude that the upper and lower metamorphic sequences developed independently but coupled with each other before ~19 Ma as a result of dextral reverse tectonic movement.     

Occurrence of Two Distinct Types of Tissue Inhibitors of Metallo-proteinases-2 in Fugu rubripes

In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 （TIMP-2） from Japanese puffer fish Fugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAs are composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteine residues, which might form six disulfide bonds as in other animals＇ TIMP-2s. Reverse-transcribed polymerase chain reaction analysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expression patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.