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31.
目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法:将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组,各组予不同药物孵育,采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率,筛选出抑制率相对较高且生长状态良好的细胞;应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变,采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用,并呈现出良好的浓度-效应相关性,与对照组比较,随着浓度的增加,细胞的增殖抑制率上升(P<0.01);Hoechst染色结果显示,与对照组相比,共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象,证明有大量的细胞出现凋亡;Western Blot结果显示,与对照组相比,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖,并诱导其凋亡,其作用机制可能与上调Bax、下调Bcl-2有关。 相似文献
32.
饱和软土压缩试验时,经常出现压缩曲线“反常”、压缩系数“倒大”的现象。它是土的原始结构发生破坏前后的不同压缩性的客观反映。饱和软土的结构力很微弱,唯质量好的原状土才能见到这种“反常”。应从成孔、取样、测试及资料整理等多个环节保证其工程意义。 相似文献
33.
本文主要对加密和身份认证技术进行了简要分析,并就如何利用证书解决WEB应用中 的信息对传输的保密性、完整性和不可否认性以及用户身份认证问题提出了安全解决 方案。 相似文献
34.
贵州雾的时空分布特征 总被引:2,自引:0,他引:2
采用贵州84个测站累计44a的雾资料,分别从各站多年平均雾日、各站累年出现雾日最多的月份及日数、各站雾最长持续时间、白天雾与夜间雾的概率分布等要素入手,分析贵州雾的时空分布气候特征. 相似文献
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Fuzzy neural network models for liquefaction prediction 总被引:1,自引:0,他引:1
Integrated fuzzy neural network models are developed for the assessment of liquefaction potential of a site. The models are trained with large databases of liquefaction case histories. A two-stage training algorithm is used to develop a fuzzy neural network model. In the preliminary training stage, the training case histories are used to determine initial network parameters. In the final training stage, the training case histories are processed one by one to develop membership functions for the network parameters. During the testing phase, input variables are described in linguistic terms such as ‘high’ and ‘low’. The prediction is made in terms of a liquefaction index representing the degree of liquefaction described in fuzzy terms such as ‘highly likely’, ‘likely’, or ‘unlikely’. The results from the model are compared with actual field observations and misclassified cases are identified. The models are found to have good predictive ability and are expected to be very useful for a preliminary evaluation of liquefaction potential of a site for which the input parameters are not well defined. 相似文献
37.
Samples of dune sands, surveys of the morphology and field measurements of wind velocity and direction of a simple linear dune in Taklimakan Sand Sea show that the airflow and sand flux vary with the change of wind direction on the dune surface. Decrease of the airflow stress on the lee flank does not result in much decrease of the sand flux because of the low threshold shear velocities and the airflow conditions. There are no significant relations between the sand flux on the lee flank and the angle of incidence of the airflow. The low threshold shear velocities and the maintenance of the sand flux at the lee flank are the main mechanisms keeping the linear shape of the dunes. Measurements of the sand flux shows that it reaches a maximum on the crest of the dune. The grain size of the transported sands has some differences compared to that of the dune surface. The sands transported are finer than that on the dune surface, but better sorted under the influence of the medium to low wind activity. The field experiment results exhibit that it is possible for the dunes to be shaped as linear dunes during the processes of accumulation and elongation. 相似文献
38.
We use 23298 Pn arrival-time data from Chinese national and provincial earthquake bulletins to invert fine structure of Pn velocity and anisotropy at the top of the mantle beneath the Sichuan-Yunnan and its adjacent region. The results suggest that the Pn velocity in this region shows significant lateral variation; the Pn velocity varies from 7.7 to 8.3 km/s. The Pn-velocity variation correlates well with the tectonic activity and heat flow of the region. Low Pn velocity is observed in southwest Yunnan, Tengchong volcano area, and the Panxi tectonic area. These areas have very active seismicity and tectonic activity with high surface heat flow. On the other hand, high Pn velocity is observed in some stable regions, such as the central region of the Yangtze Platform; the most pronounced high velocity area is located in the Sichuan Basin, south of Chengdu. Pn anisotropy shows a complex pattern of regional deformation. The Pn fast direction shows a prominent clockwise rotation pattern from east of the Tibetan block to the Sichuan-Yunnan diamond block to southwest Yunnan, which may be related to southeastward escape of the Tibetan Plateau material due to the collision of the Indian Plate to the Eurasia Plate. Thus there appears to be strong correlation between the crustal deformation and the upper mantle structure in the region. The delay times of events and stations show that the crust thickness decreases from the Tibetan Plateau to eastern China, which is consistent with the results from deep seismic sounding. 相似文献
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