<Emphasis Type="Italic">Dracograllus trukensis</Emphasis> sp. nov. (Draconematidae: Nematoda) from a seagrass bed (<Emphasis Type="Italic">Zostera</Emphasis> spp.) in Chuuk Islands,Micronesia, Central Western Pacific Ocean

A new species of free-living marine draconematid nematode, Dracograllus trukensis sp. nov., is described based on the specimens collected from the sediments of a intertidal seagrass bed from Chuuk Islands, Micronesia. Dracograllus trukensis sp. nov. differs from other species of the genus by the combination of the following characteristics: the presence of numerous minute spiny ornamented body cuticular annules in both sexes, eight cephalic adhesion tubes inserted on the head capsule in both sexes, the presence of stiff posteriorly directed setae anterior to posterior adhesion tubes in both sexes, the shape (large, elongated, open loop-shaped in male and large, elongated, closed loop-shaped in female) and position (longer ventral arm extending to the first body annule in male) of amphideal fovea, shorter spicule length (34–42 μm), the presence of sexual dimorphism in shape and length of the non-annulated tail terminus, and number of posterior sublateral adhesion tubes (10 in male and 13–15 in female) and posterior subventral adhesion tubes (8–10 in male and 9–11 in female). A comparative table on the biogeographical and ecological characteristics of the species of Dracograllus is presented. This is the first taxonomic report on the genus Dracograllus from Chuuk Islands, Micronesia, central western Pacific Ocean.     

Acetylthiocholine (ATC)--cleaving cholinesterase (ChE) activity as a potential biomarker of pesticide exposure in the Manila clam, Ruditapes philippinarum, of Korea

The acetylthiocholine (ATC) - cleaving cholinesterase (ChE) activity in Manila clam, which is widely distributed throughout the coastal environment of Korea, was assayed as a potentially useful biomarker of organophosphorous pesticides (OPs). A clear dose-response relationship was determined between inhibited ChE in adductor muscle of clams and four OPs (methidathion, chlorpyrifos, diazinon, IBP) which are heavily used OPs in Korea. The measured EC(50)-24?h values of methidathion, chlorpyrifos, diazinon, and IBP for Ruditapes philippinarum were 7.16?μg?l(-1), 0.34?mg?l(-1), 3.01?mg?l(-1), and 3.41?mg?l(-1), respectively. In field studies, ChE activity in Manila clams collected from 23 stations in the mid-western coastal region demonstrated spatial variation with statistical differences. These results suggest that ChE activity in R. philippinarum is a potential biomarker for assessing organophosphorous pesticide contamination in coastal environments.     

Practical considerations for the segmented-flow analysis of nitrate and ammonium in seawater and the avoidance of matrix effects

In this study we describe measures taken in our laboratory to improve the long-term precision of nitrate and ammonia analysis in seawater using a microflow segmented-flow analyzer. To improve the nitrate reduction efficiency using a flow-through open tube cadmium reactor (OTCR), we compared alternative buffer formulations and regeneration procedures for an OTCR. We improved long-term stability for nitrate with a modified flow scheme and color reagent formulation and for ammonia by isolating samples from the ambient air and purifying the air used for bubble segmentation. We demonstrate the importance of taking into consideration the residual nutrient content of the artificial seawater used for the preparation of calibration standards. We describe how an operating procedure to eliminate errors from that source as well as from the refractive index of the matrix itself can be modified to include the minimization of dynamic refractive index effects resulting from differences between the matrix of the samples, the calibrants, and the wash solution. We compare the data for long-term measurements of certified reference material under two different conditions, using ultrapure water (UPW) and artificial seawater (ASW) for the sampler wash.     

Tensile strength equation for HSS bracing members having slotted end connections

In the previous study, the authors investigated the effect of w/t ratios on the behaviour of bracing members under symmetric cyclic loading in compression and tension. In this study, 11 bracing members with slotted end sections made of cold‐formed square hollow structural sections (HSS) were tested. The w/t ratios ranged from 8 to 28. Unlike the test results of other former studies obtained under compression‐oriented cyclic loading, the results of this study showed that bracing members having a smaller w/t ratio (<14) had less deformation and less energy dissipation capacity, and a shorter fracture life compared with other specimens. Such inferior behaviour resulted from early fracture at the slotted end section. This study compares tensile strength obtained from the design equations in the AISC LRFD manual and Eurocode 3 using the actual strengths of the tested specimens. This study found that for preventing early fracture in HSS bracing members, design fracture strength should be larger than design yield strength. Design strength equations are proposed for bracing members in special concentrically braced frames (SCBF). The proposed design equations are verified by experimental tests conducted under symmetric cyclic loading in tension and compression using two HSS bracing members designed according to the proposed equation. Copyright © 2007 John Wiley & Sons, Ltd.     

Stable C and N isotopic composition of sinking particles and zooplankton over the southeastern Bering Sea shelf

Stable carbon and nitrogen isotopic composition of zooplankton, suspended particulate organic matter (SPOM), and sinking particles collected using sediment traps were measured for samples obtained from the southeastern Bering Sea middle and outer shelf during 1997–1999. The quantity of material collected by the middle shelf sediment trap was greater in both spring and late summer and fall than in early and mid-summer. The δ¹⁵N of SPOM, sinking material and zooplankton showed greater inter-annual variability at the middle shelf site (M2) than at the outer shelf site (M3). Zooplankton and sinking organic matter collected by M2 sediment traps became more depleted in ¹⁵N from 1997 through 1999, associated with a change from unusually warm to unusually cold conditions. Suspended and sinking organic matter and zooplankton collected from M3 decreased only slightly in δ¹⁵N from 1998 to 1999. SPOM, zooplankton, and sediment trap samples collected at M2 were usually enriched in δ¹⁵N and δ¹³C over those from M3. However, in 1999 sediment trap samples from the middle shelf were enriched in ¹³C over M3 material, but the δ¹⁵N of samples from the two sites was similar. The geographic pattern could be explained greater productivity over the middle shelf, associated with either isotopically heavy nitrogen being regenerated from sediments, or with utilization of a greater fraction of the available inorganic nitrogen pool during most years.     

Update of the precision geoid determination in Korea

From the late 1990s, many studies on local geoid construction have been made in South Korea. However, the precision of the previous geoid has remained about 15 cm due to distribution and quality problems of gravity and GPS/levelling data. Since 2007, new land gravity data and GPS/levelling data have been obtained through many projects such as the Korean Land Spatilaization, Unified Control Point and Gravity survey on the Benchmark. The newly obtained data are regularly distributed to a certain degree and show much better improvement in their quality. In addition, an airborne gravity survey was conducted in 2008 to cover the Korean peninsula (South Korea only). Therefore, it is expected that the precision of the geoid could be improved. In this study, the new South Korean gravimetric geoid and hybrid geoid are presented based on land, airborne, ship‐borne, altimeter gravity data, geopotential model and topographic data. As for the methodology, the general remove‐restore approach was applied with the best chosen parameters in order to produce a precise local geoid. The global geopotential model EGM08 was used to remove the low‐frequency components using degree and order up to 360 and the short wavelength part of the gravity signal was dealt with by using the Shuttle Radar Topography Mission data. The parameters determined empirically in this study include for Stokes’ integral 0.5° and for Wong‐Gore kernel 110–120°, respectively and 10 km for both the Bjerhammar sphere depth and attenuation factor. The final gravimetric geoid in South Korea ranges from 20–31 m with a precision of 5.45 cm overall compared to 1096 GPS/levelling data. In addition, the South Korean hybrid geoid produces 3.46 cm and 3.92 cm for degrees of fitness and precision, respectively and a better statistic of 2.37 cm for plain and urban areas was achieved. The gravimetric and hybrid geoids are expected to improve further when the refined land gravity data are included in the near future.     

Hydraulic Fracturing In Situ Stress Estimations in a Potential Geothermal Site,Seokmo Island,South Korea

We conducted hydraulic fracturing (HF) in situ stress measurements in Seokmo Island, South Korea, to understand the stress state necessary to characterize a potential geothermal reservoir. The minimum horizontal principal stress was determined from shut-in pressures. In order to calculate the maximum horizontal principal stress (S _Hmax) using the classical Hubbert–Willis equation, we carried out hollow cylinder tensile strength tests and Brazilian tests in recovered cores at depths of HF tests. Both tests show a strong pressure rate dependency in tensile strengths, from which we derived a general empirical equation that can be used to convert laboratory determined tensile strength to that suitable for in situ. The determined stress regime (reverse-faulting) and S _Hmax direction (ENE–WSW) at depths below ~300 m agrees with the first order tectonic stress. However the stress direction above ~300 m (NE–SW) appears to be interfered by topography effect due to a nearby ridge. The state of stress in Seokmo Island is in frictional equilibrium constrained by optimally oriented natural fractures and faults. However, a severe fluctuation in determined S _Hmax values suggests that natural fractures with different frictional coefficients seem to control stress condition quite locally, such that S _Hmax is relatively low at depths where natural fractures with low frictional coefficients are abundant, while S _Hmax is relatively high at depths where natural fractures with low frictional coefficients are scarce.     

Multivariate statistical analysis to identify the major factors governing groundwater quality in the coastal area of Kimje,South Korea

The main purpose of this study is to identify the major factors affecting groundwater quality by means of multivariate statistical analysis of the physico‐chemical compositions. Cluster analysis results show that the groundwater in the study area is classified into four groups (A, B, C and D), and factor analysis indicates that groundwater composition, 81·9% of the total variance of 17 variables, is mainly affected by three factors: seawater intrusion, microbial activity and chemical fertilizers. These results might be related to the geographical characteristics of the study area. The main influence on groundwater in groups B, C and D, which are close to the Yellow Sea and contain reclaimed areas, is the seawater intrusion by the present seawater, the trapped seawater, and microbial activity. Group A, however, has been used for agriculture for a long time, and thus groundwater in this group has been largely affected by chemical fertilizers. As groundwater flows from group A to group D according to its path, the governing factor of the groundwater quality gradually changes from chemical fertilizers to microbial activity and seawater intrusion. Copyright © 2004 John Wiley & Sons, Ltd.