Experimental research on reservoir sensitivity to stress and impacts on productivity in Kela 2 Gas Field

Kela 2 Gas Field, with high formation pressure (74.35MPa), high pressure coefficient (2.022) and difficulty of potential test and evaluation, is the largest integrated proved dry gas reservoir in China so far and the principal source for West-East Gas Development Project. In order to correctly evaluate the elastic-plastic deformation of rocks caused by the pressure decline during production, some researches, as the experiment on reservoir sensitivity to stress of gas filed with abnormal high pressure, are made. By testing the rock mechanic properties, porosities and permeabilities at different temperature and pressure of 342 core samples from 5 wells in this area, the variations of petro-physical properties at changing pressure are analyzed, and the applicable inspection relationship is concluded. The average productivity curve with the reservoir sensitivity to stress is plotted on the basis of the research, integrated with the field-wide productivity equation. The knowledge lays a foundation for the gas well productivity evaluation in the field and the gas field development plan, and provides effective techniques and measures for basic research on the development of similar gas fields.     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

Hoek—Brown准则在主应力平面表示形式的讨论

Hoed-Brown准则是国际上有名的岩体经验强度准则，考虑了应力状态对岩体破坏特性的影响，在系统介绍H－B准则在主应力平面p,q表示形式的基础上，重点对其理论意义和实际应用中的关键问题进行分析讨论，以加深对该准则的理解。     

我国科学数据共享现状的调查

本文是科学数据共享政策法规体系研究系列文章的收笔之作.撰写本文的目的,就是为说明科学数据共享政策法规体系的研究是建立在大量的基础调研之上.调查研究是谋事之基、成事之道.从实践中寻求解决现实问题的方法,才能制定出切实可行的政策、法规,调查研究是制定政策、法规的客观依据.为使制订政策、 规章制度适应社会经济发展和变化的新情况,就深入开展调查研究,认真听取各方面的意见,深入研究相应的管理规律和运行机制.本文详述了调查过程、调查方法,以佐证政策法规研究不能脱离实际.     

Relationships between Interannual and Intraseasonal Variations of the Asian-Western Pacific Summer Monsoon Hindcasted by BCC_CSM1.1（m）

Using hindcasts of the Beijing Climate Center Climate System Model, the relationships between interannual variability （IAV） and intraseasonal variability （ISV） of the Asian-western Pacific summer monsoon are diagnosed. Predictions show reasonable skill with respect to some basic characteristics of the ISV and IAV of the western North Pacific summer monsoon （WNPSM） and the Indian summer monsoon （ISM）. However, the links between the seasonally averaged ISV （SAISV） and seasonal mean of ISM are overestimated by the model. This deficiency may be partially attributable to the overestimated frequency of long breaks and underestimated frequency of long active spells of ISV in normal ISM years, although the model is capable of capturing the impact of ISV on the seasonal mean by its shift in the probability of phases. Furthermore, the interannual relationships of seasonal mean, SAISV, and seasonally averaged long-wave variability （SALWV; i.e., the part with periods longer than the intraseasonal scale） of the WNPSM and ISM with SST and low-level circulation are examined. The observed seasonal mean, SAISV, and SALWV show similar correlation patterns with SST and atmospheric circulation, but with different details. However, the model presents these correlation distributions with unrealistically small differences among different scales, and it somewhat overestimates the teleconnection between monsoon and tropical central-eastern Pacific SST for the ISM, but underestimates it for the WNPSM, the latter of which is partially related to the too-rapid decrease in the impact of E1 Nifio-Southern Oscillation with forecast time in the model.     

正态分布检验在地下水检测质量控制中的应用

对地下水调查中37种必测组分检测过程的替代物进行筛选,应用D’Agostino法对替代物的测量结果进行正态性检验。利用正态分布函数"3σ"原则,定义了各替代物质量控制指标的有效区间。应用正态函数随机变量的数学期望和方差,计算得到各替代物有效区间内的正态函数分布概率均大于97%,替代物过程能力指数计算结果表明,二溴氟代甲烷、甲苯-d8、对溴氟苯、1,2-二氯苯-d4、氟苯等挥发性有机污染物分析过程选用的替代物质量控制过程处于稳定的监控状态;2,4,5,6-四氯间二甲苯、二丁基氯菌酸酯、p-三联苯、十氯联苯等半挥发性有机污染物分析过程选用的替代物监控指标分析过程能力不足。建议参考美国EPA方法中有关监控指标质量控制区间的要求,适当放宽对半挥发性有机物检测流程中选用的替代物质量控制区间的有效范围。正态分布在地下水检测过程质量控制中的应用,为实验室检测过程的质量管理提供了科学、有效的数学依据。     

淮北地区一次强风暴的弓形回波分析

2005年7月16日淮北地区发生了一次强风暴天气过程。主要使用新建成的阜阳CINRAD-SA多普勒天气雷达观测资料,辅以天气图、本站高空观测、地面观测资料,对此过程进行了综合分析。结果表明：这是一次以灾害性大风为主、有雨暴相伴随、局部还有冰雹发生的强对流天气过程。影响系统是一条中-β尺度的弓形飑线回波带。弓形回波带是由更早些时间出现的弧形的阵风锋触发而成的。中层干冷空气的侵入加强了对流云体中的下沉气流和低层出流,形成阵风锋。在如何综合应用如此丰富的雷达产品做好临近预报方面作了初步的尝试。     

Seasonal change in body length of important small copepods and relationship with environmental factors in Jiaozhou Bay,China

Differences among species in prosome length and in species’ response to environmental factors do exist. Therefore, it is useful to examine prosome length for different copepod species in variable environments. Seasonal variations in prosome length of four small copepods and their copepodite stages in the Jiaozhou Bay were compared and the relative influence of temperature, salinity, and chlorophyll concentration were examined. Two peaks were found in the mean prosome length of Paracalanus parvus (in early winter and May). For Acartia bifilosa, the maximum values of all copepodites occurred mainly from February to April, and decreased to the bottom in July. Prosome length of Acartia pacifica peaked when it first appeared in June, then reached to the minimum in July. Parvocalanus crassirostris only appeared from late summer to autumn and the mean prosome length showed no clear changes. Correlations of adult prosome length with environmental factors were evaluated. For the four species, temperature was negatively correlated to prosome length except for P. crassirostris. But the different species varied markedly in their responds to temperature. A. bifilosa showed a more definite trend of size variation with temperature than P. parvus and A. pacifica. Correlations of prosome length with salinity were significantly positive for almost all the small copepods. The relationship between chlorophyll concentration and prosome length was complicated for these copepods, but for P. parvus, chlorophyll concentration was also an important affecting factor. Furthermore, investigation needs to be done on food quality for some copepod. These results are essential to estimate the biomass and the production, and to understand these small copepods’ population dynamics in this human-affected bay.     

基于神经网络优化算法的海洋动态缓波型立管设计

柔性立管连接海上平台和水下生产系统,缓波型构型可以减轻其受到的顶部张力和疲劳损伤,触地点处系链对于固定水下立管起着重要作用,然而加装系链的缓波型立管系统设计更为复杂。通过缓波型基本理论计算出合理的立管初始状态,之后使用立管分析软件OrcaFlex,在充分考虑环境因素、船体结构、立管材料和线型以及重力块、浮力块、系链等配置参数的情况下,建立缓波型立管系统有限元模型,通过静动态分析验证其满足设定的关于立管构型、张力、弯曲半径,系链张力和FPSO偏移量5个约束条件。结合神经网络优化算法和遗传算法制定出针对文中立管系统的优化算法并通过MATLAB编程将浮力块数量优化至最少,之后基于L-M算法构建神经网络模型,通过迭代训练提升精度,得到最终参数优化结果。通过对比优化前后静动态分析结果可知：优化后浮力块数量大幅减少,立管最大有效张力大幅减小,而悬挂段最低点深度有一定程度的增大,整体构型更趋向于合理的缓波型构型。     

层状盐岩造腔仿真软件开发及其实用性验证

国内层状盐岩具有夹层多、不溶物含量高等特点,现有造腔软件不能很好地模拟国内层状盐岩水溶造腔过程。基于流体力学和化学动力学等理论,建立能够描述盐岩造腔过程的数学模型,并提出了夹层垮塌判据。结合国内层状盐岩水溶造腔工艺特征,开发出能模拟含夹层盐岩水溶造腔的软件Salt Cavern Builder V1.0。该软件可模拟纯盐岩、多夹层盐岩的水溶造腔过程,能较好地反映出造腔方案在现场造腔过程中每个阶段的实施效果。通过大尺寸型盐造腔相似试验,模拟无夹层和含单一夹层的腔体形状扩展过程。基于相似理论基础,在现场造腔工艺条件下,造腔软件模拟出的结果与试验得到的结果接近,验证了该软件的实用性。