Biomarkers for endocrine disruptors in three species of Mediterranean large pelagic fish

The hypothesis that Mediterranean top predator species, such as large pelagic fish, are potentially at risk due to endocrine disrupting chemicals (EDCs), is investigated. The potential estrogenic effects of PHAHs in three fish species of commercial interest, the top predators bluefin tuna (Thunnus thynnus thynnus), swordfish (Xiphias gladius), and Mediterranean spearfish (Tetrapturus belone), were investigated using vitellogenin (Vtg), zona radiata proteins (Zrp) and mixed function oxidases (EROD, BPMO) as diagnostic tools. High induction of Vtg and Zrp was detected by western blot and ELISA techniques in adult males of X. gladius and T. thynnus thynnus, suggesting that these species are at high toxicological risk in the Mediterranean sea. Comparison of BPMO and EROD activities in the three species indicated, both in male and female, much higher MFO activity in bluefin tuna. This data suggests high exposure of this species to lipophilic xenobiotic contaminants in the Mediterranean environment.     

Induction of cytochrome P4501A and biliary PAH metabolites in European eel Anguilla anguilla: Seasonal, dose- and time-response variability in field and laboratory conditions

Induction of cytochrome P4501A in fish is a well-known indicator of exposure to polycyclic aromatic hydrocarbons (PAHs) and determination of PAH metabolites in bile by fixed wavelength fluorescence (FF) or synchronous fluorescence spectroscopy (SFS), has become an useful method in monitoring programs. In this work the relationship between cytochrome P4501A (EROD activity) and levels of biliary PAH metabolites was measured in the European eel Anguilla anguilla, in both field and laboratory conditions: organisms were sampled on a seasonal basis from the Orbetello lagoon (Tuscany) to characterize the natural variability of these biological parameters, while in laboratory eels were intraperitoneally injected with benzo[a]pyrene to investigate temporal and dose-dependent induction patterns. Results showed that induction of cytochrome P450 and accumulation of PAHs metabolites in bile are not necessarily correlated either in field, or in laboratory investigations; different seasonal changes were measured in natural conditions and slight variations in dose and time response patterns were also obtained in laboratory exposures.     

Evaluating the effects of protection on two benthic habitats at Tavolara-Punta Coda Cavallo MPA (North-East Sardinia, Italy)

In this paper, two benthic habitats have been investigated in a fully protected site and two control sites at the Tavolara-Punta Coda Cavallo marine protected area (MPA) (NE Sardinia). Overall, a protection effect on the benthic assemblages was highlighted in the shallow subtidal (5 m deep) rather than at intertidal algal turf habitat. Structure of assemblages at the shallow subtidal habitat is different in the fully protected site vs. controls, while this was not true for the intertidal habitat. At the subtidal, this finding is probably linked to indirect effects due to an increase of consumers in the protected site (e.g., sea urchins), while the lack of direct impacts in the intertidal at control sites is indicative of very similar assemblages. Cover of encrusting algae was significantly higher at the subtidal protected site suggesting a possible higher grazing pressure. Possible causes underlying the inconsistency of results obtained between habitats include the possibility that trophic cascade effects have a different influence at different heights on the shore. The need to estimate the interconnection among benthic habitats through trophic links is also highlighted to provide an estimate of the vulnerability to protection of various habitats.     

Trajectory analysis at intersections for traffic rule identification

In this paper,we focus on trajectories at intersections regulated by various regulation types such as traffic lights,priority/yield signs,and right-of-way rules.We test some methods to detect and recognize movement patterns from GPS trajectories,in terms of their geometrical and spatio-temporal components.In particular,we first find out the main paths that vehicles follow at such locations.We then investigate the way that vehicles follow these geometric paths(how do they move along them).For these scopes,machine learning methods are used and the performance of some known methods for trajectory similarity measurement(DTW,Hausdorff,and Fréchet distance)and clustering(Affinity propagation and Agglomerative clustering)are compared based on clustering accuracy.Afterward,the movement behavior observed at six different intersections is analyzed by identifying certain movement patterns in the speed-and time-profiles of trajectories.We show that depending on the regulation type,different movement patterns are observed at intersections.This finding can be useful for intersection categorization according to traffic regulations.The practicality of automatically identifying traffic rules from GPS tracks is the enrichment of modern maps with additional navigation-related information(traffic signs,traffic lights,etc.).     

Long‐term changes in hydroid (Cnidaria,Hydrozoa) assemblages: effect of Mediterranean warming?

Marine hydroids are markedly seasonal in temperate seas, being extremely sensitive to climatic changes disrupting seasonal patterns. Modifications in the composition, seasonality, bathymetric distribution and reproductive period of hydroid assemblages are useful to evaluate the influence of global warming on the marine ecosystem. The hydroids on the rocky cliff of the Portofino Promontory (Ligurian Sea, Italy) were carefully studied between 1976 and 1983; in particular, in 1980 the study was carried out along a vertical transect. The hydroids were sampled again throughout 2004, with the same techniques and along the same transect. Species diversity decreased slightly in the 2004 survey. Some species present in 1980 had disappeared in 2004, but other species with southern affinity, never recorded from the area, became abundant in 2004. Species that were present in summer in the first period were also present in winter in the second one. Furthermore, shallow summer species widened their bathymetric distribution, reaching deeper levels. These data strongly suggest that the Portofino hydroid assemblage reacted to the water temperature increase found in the Mediterranean Sea.     

Zonal jets and cyclone–anticyclone asymmetry in decaying rotating turbulence: laboratory experiments and numerical simulations

The problem of zonal jet formation and cyclone–anticyclone asymmetry in decaying rotating turbulence is addressed using both laboratory experiments and numerical simulations with a high-resolution shallow water model in a spherical geometry. Experiments are performed at different Rossby and Froude numbers and applying a rigid wall as meridional boundary in the numerical scheme to mimic the experimental apparatus. The formation of a zonally banded flow pattern, i.e. meridionally confined easterly/westerly jets, has observed; both experimental and numerical results confirmed that this tendency is favoured by high-planetary vorticity gradients. Also, in the experiments characterized by large rotation speeds and small Rossby deformation radius, an initial symmetric distribution of relative vorticity is found to evolve towards a dominance of anticyclonic structures, indicating a breaking of the cyclone–anticyclone symmetry. This aspect has deepened by numerically analysing the sensitivity of the temporal variations of the asymmetry index with respect to the position of the meridional confinement as well as the effect of relaxing the divergence of the fluid (i.e. non-divergent case) to zero. Results suggested that experiments characterized by the higher rotation speed and the lower fluid thickness are better reproduced by a divergent model with a high-latitude meridional boundary.     

Relative contribution of foliar and fine root pine litter to the molecular composition of soil organic matter after in situ degradation

The influence of litter quality on soil organic matter (SOM) stabilization rate and pathways remains unclear. We used ¹³C/¹⁵N labeled litter addition and Curie-point pyrolysis gas chromatography–mass spectrometry combustion-isotope ratio mass spectrometry (Py–GC–MS–C–IRMS) to explore the transformation of litter with different composition and decay rate (ponderosa pine needle vs. fine root) to SOM during 18 months in a temperate conifer forest mineral (A horizon) soil. Based on ¹³C Py–GC–MS–C–IRMS the initial litter and bulk soil had ∼1/3 of the total pyrolysis products identified in common. The majority was related either to carbohydrates or was non-specific in origin. In bulk soil, carbohydrates had similar levels of enrichment after needle input and fine root input, while the non-specific products were more enriched after needle input. In the humin SOM fraction (260 yr C turnover time) we found only carbohydrate and alkyl C-derived compounds and greater ¹³C enrichment in the “carbohydrate” pool after fine root decomposition. ¹⁵N Py–GC–MS–C–IRMS of humic substances showed that root litter contributed more than needle litter to the enrichment of specific protein markers during initial decomposition.We found little evidence for the selective preservation of plant compounds considered to be recalcitrant. Our findings suggest an indirect role for decomposing plant material composition, where microbial alteration of fine root litter seems to favor greater initial stabilization of microbially derived C and N in SOM fractions with long mean turnover times, such as humin, compared to needles with a faster decay rate.     

One Year of Surface-Based Temperature Inversions at Dome C, Antarctica

In 2005 the Study of Stable Boundary Layer Environment at Dome C (STABLEDC) experimental campaign was conducted at the plateau station of Concordia at Dome C, Antarctica. Temperature profiles measured with a microwave radiometer were used to study the characteristics of surface-based temperature inversions over the course of a year. Statistics of temperature profiles for every month are discussed; the difference between daytime and nocturnal cases observed during the summer months disappears during winter. Surface-based temperature inversions occurred in 70 % of the time during summer, and almost all of the time during winter. During winter the occurrence of warming events leads to a decrease in the temperature difference between the top and the base of the inversion (i.e. the inversion strength). The inversion strength maxima ranged between $3\,^{\circ }\mathrm{C}$ (December) and $35\,^{\circ }\mathrm{C}$ (August) corresponding to gradients of 0.1 and $0.3\,^{\circ }\mathrm{C}\, \mathrm{m}^{-1}$ , respectively. The average surface-based inversion height presents a daily cycle during the summer months with values up to 200 m in the morning hours, while it affects a layer always deeper than 100 m during the winter months. The relationships between inversion strength and the downward longwave radiative flux, absolute temperature, and wind speed are examined. The inversion strength decreases as the longwave radiation increases. A clear anti-correlation between inversion strength and near-surface temperature is evident throughout the year. During the winter, the largest inversion strength values were observed under low wind-speed conditions; in contrast, a clear dependence was not found during the summer.     

Release of toxic elements from rocks and mine wastes at the Furtei gold mine (Sardinia, Italy)

The Furtei gold mine in Sardinia (Italy) exploits a volcanic-hosted high-sulphidation epithermal deposit. Large amounts of materials derived from exploitation are present in open pits, waste rock dumps and cyanidation tailings impoundment. Mineralized rocks in outcrops and waste dumps contain significant amounts of sulphides (mainly pyrite and enargite). These materials have a high potential for acid drainage generation and release of toxic elements (notably Cu and As, but also Al, Ni, Co and Cd) as pointed out by laboratory leaching tests and in agreement with chemical composition of waters draining the mining area, that show pH as low as 2, up to 180 mg/L Cu, up to 5 mg/L As, and up to 788 mg/L Al. On the other hand, leaching solutions and waters interacting with mineral assemblages of the propylitic alteration zone (mainly composed of chlorite, quartz, and calcite, with relic magmatic plagioclase) show higher pH, and lower metal loads. Leachates from cyanidation tailings show variable pH (between 6.2 and 9.7, depending on sulphide content in tailings); cyanide concentration varies between 110 µg/L and about 3 mg/L, whereas contents of toxic elements in leachates are, with the exception of Hg, within the limits of Italian regulations for non-dangerous industrial wastes. Reclamation plans provide for confinement of tailings within specific repositories. This measure should effectively reduce the environmental impact of these materials. Reclamation plans should also include an adequate management of other high-sulphide wastes.