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This paper systematically studies the statistical diagnosis and hypothesis testing for the semiparametric linear regression model according to the theories and methods of the statistical diagnosis and hypothesis testing for parametric regression model.Several diagnostic measures and the methods for gross error testing are derived.Especially,the global and local influence analysis of the gross error on the parameter X and the nonparameter s are discussed in detail;at the same time,the paper proves that the d... 相似文献
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Galectins,a family of ?-galactoside-binding proteins,participate in both innate immunity and adaptive immunity.This study identified one novel galectin-related protein from half-smooth tongue sole Cynoglossus semilaevis,which was designated as Cs GRP.The full-length c DNA of its encoding gene was 785 bp in length with a 528 bp open reading frame encoding a putative protein of 176 amino acids.The deduced Cs GRP contained a putative 131-aa galactoside-binding lectin domain and 3 critical residues responsible for carbohydrate binding(R~(93),W~(109) and R~(114)).Genomic structural analysis revealed that Cs GRP consisted of five exons and four introns.Cs GRP showed 68% similarity with Poecilia latipinna GRP and 67% similarity with Stegastes partitus GRP.Cs GRP showed the highest expression level in liver,although its expression was detected in all tested tissues.When challenged with Vibrio harveyi,the expression of Cs GRP was significantly down-regulated in liver(P 0.05).In addition,we found that in spleen and kidney of C.semilaevis,the Cp G island of Cs GRP showed significantly higher(P 0.05) methylation level in disease-resistant family of C.semilaevis(DR-Cs) than in disease-susceptible ones(DS-Cs).Our results suggested that Cs GRP may play important roles in the immune response of C.semilaevis.Moreover,DNA methylation patterns provided valuable data for understanding the relationship between epigenetic regulation and immunity,which would assist the animal genetic research and improve the animal breeding in the future. 相似文献
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Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis) 总被引:3,自引:1,他引:2
A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus. 相似文献
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利用地震层析成象技术确定横向非均匀介质中的速度分布和界面位置 总被引:2,自引:0,他引:2
本文提出一种横向非均匀介质中的地震层析成象方法,由地震走时资料确定介质的速度分布和界面位置。计算中采用了参数分离、变阻尼、奇异值分解等技术,方程的解较稳定,且可得到解的分办。除反射波外,该方法还可同时利用分析波的资料。因此,适宜于折射及宽角度反射地震测深剖面的资料处理。数字试验结果表明,在模型内部,该方法能得到可靠的反演结果。在边界附近效果较差,受初始值影响较大。 相似文献
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