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71.
Wenzhi Zhao Shuichang Zhang Feiyu Wang Jianping Chen Zhongyao Xiao Fuqing Song 《Organic Geochemistry》2005,36(12):1602-1616
Oil and gas exploration in eastern Tarim Basin, NW China has been successful in recent years, with several commercial gas accumulations being discovered in a thermally mature to over-mature region. The Yingnan2 (YN2) gas field, situated in the Yingnan structure of the Yingjisu Depression, produces gases that are relatively enriched in nitrogen and C2+ alkanes. The δ13C1 (−38.6‰ to −36.2‰) and δ13C2 values (−30.9‰ to −34.7‰) of these gases are characteristic of marine sourced gases with relatively high maturity levels. The distributions of biomarkers in the associated condensates suggest close affinities with the Cambrian–Lower Ordovician source rocks which, in the Yingjisu Sag, are currently over-mature (with 3–4%Ro). Burial and thermal maturity modeling results indicate that paleo-temperatures of the Cambrian–Lower Ordovician source rocks had increased from 90 to 210 °C during the late Caledonian orogeny (458–438 Ma), due to rapid subsidence and sediment loading. By the end of Ordovician, hydrocarbon potential in these source rocks had been largely exhausted. The homogenization temperatures of hydrocarbon fluid inclusions identified from the Jurassic reservoirs of the YN2 gas field suggest a hydrocarbon emplacement time as recent as about 10 Ma, when the maturity levels of Middle–Lower Jurassic source rocks in the study area were too low (<0.7%Ro) to form a large quantity of oil and gas. The presence of abundant diamondoid hydrocarbons in the associated condensates and the relatively heavy isotopic values of the oils indicate that the gases were derived from thermal cracking of early-formed oils. Estimation from the stable carbon isotope ratios of gaseous alkanes suggests that the gases may have been formed at temperatures well above 190 °C. Thus, the oil and gas accumulation history in the study area can be reconstructed as follows: (1) during the late Caledonian orogeny, the Cambrian–Lower Ordovician marine source rocks had gone through the peak oil, wet gas and dry gas generation stages, with the generated oil and gas migrating upwards along faults and fractures to form early oil and gas accumulations in the Middle–Upper Ordovician and Silurian sandstone reservoirs; (2) since the late Yanshanian orogeny, the early oil accumulations have been buried deeper and oil has undergone thermal cracking to form gas; (3) during the late Himalayan orogeny, the seals for the deep reservoirs were breached; and the gas and condensates migrated upward and eventually accumulating in the relatively shallow Jurassic reservoirs. 相似文献
72.
Correlation between magnetic susceptibility and heavy metals in urban topsoil: a case study from the city of Xuzhou, China 总被引:7,自引:0,他引:7
Anthropogenic influence, mainly due to urban and industrial activities and traffic exhaust, may affect urban topsoil via atmospheric contamination and solid waste. Magnetic susceptibility measurements were conducted on 21 urban topsoil samples from the city of Xuzhou, China. High intensities of magnetic susceptibility were detected in the majority of the samples. SEM analysis shows that magnetic minerals are in the form of spherules and mainly due to anthropogenic inputs. The heavy metals Pb, Cu, Zn, Se, Sc, Mo, Fe, and Bi show strong correlations with magnetic susceptibility, and Ag, Ba, Cd, Ni, Cr, Sb, and Sn, on the other hand, show a weak correlation with magnetic susceptibility. Whereas, of these metals studied, only Hg has no significant correlation with the susceptibility. The Tomlinson pollution load index (PLI) also shows significant correlation with the susceptibility (χ). The present study shows that magnetic susceptibility is a fast, inexpensive, and non-destructive method for the detection and mapping of contaminated soils. 相似文献
73.
74.
Identification of phase and sex-related ISSR markers of red algaGracilaria lemaneiformis 总被引:1,自引:0,他引:1
Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 and SF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank. 相似文献
75.
76.
泾县地震台地磁核旋观测干扰排除分析 总被引:1,自引:1,他引:0
地磁观测是地震预报中重要的前兆手段之一。识别干扰,排除干扰,对保证观测资料的可靠尤为重要。我们根据泾县地震台地磁核旋观测过程中遇到的问题,利用对比观测方法,对观测数据进行分析,结果表明该仪器工作正常,性能稳定,精度很高;同时指出,在观测过程中应注意的一些问题,并排除了影响泾县地震台地磁观测的干扰源。 相似文献
77.
78.
古村落社区旅游参与度及影响因素——西递、宏村、南屏比较研究 总被引:2,自引:0,他引:2
以社区家庭为视角,建立基于利益导向的社区旅游参与水平测度指标,以皖南古村落西递、宏村、南屏为实证研究对象,揭示了古村落社区旅游参与度异同及其影响机理。研究结果表明:① 古村落内部、古村落之间的旅游参与度均有不同程度的差异,归因为家庭微观因子与社区中观因子交互作用所致。② 通过家庭微观因子对家庭人均旅游收入影响的通径系数分析,得出三个景区相应的直接通径系数和间接通径系数,发现古村落家庭微观因子的影响方向趋同而影响程度分异,与问卷调查结果分析的一致性也显示了该方法的较强解释力和潜在应用价值。③ 提高古村落社区旅游参与整体水平、缓解参与度空间差异的根本途径在于"让利于民",增强社区分利能力,政府在其中扮演着重要的引导角色。 相似文献
79.
本文从当前平顶山市矿业特点及引发的问题出发,着重指出实现矿业经济可持续发展,必须加大矿业结构的调整,并从资源管理方式、资源配置、矿业秩序、地勘工作等方面作了较为详细的论述。 相似文献
80.
Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE 总被引:1,自引:0,他引:1
WEN Ruobing) SUI Zhenghong) * ZHANG Xuecheng) ZHANG Shuang) and QIN Song) ) Laboratory of Marine Genetics Breeding College of Marine Life Sciences Ocean University of China Qingdao P. R. China ) Institute of Oceanology Chinese Academy of Sciences Qingdao P.R. China 《中国海洋大学学报(英文版)》2007,6(4):373-377
Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value. 相似文献