Are there salinity stress proteins?

To establish whether osmotic stress can be separated from possible toxicant stress in Eurytemora affinis, protein synthesis was analyzed under various conditions of temperature and osmotic stress. Individual adult copepods raised at 10 ppt and 15 °C were exposed for 5 h to different temperature and salinity regimes. Newly synthesized proteins were separated and identified using SDS-PAGE and autoradiography. Copepods exposed to lower (2 and 5 ppt) and higher (15 and 20 ppt) salinities showed differences in the up- and down-regulation of specific proteins. Concurrent heat stress changed these protein patterns. These results suggest that protein profiles might be used to distinguish the effect of toxicants in a salinity gradient such as the Chesapeake Bay or in the Baltic region.     

Rummaging through Earth's Attic for Remains of Ancient Life

We explore the likelihood that early remains of Earth, Mars, and Venus have been preserved on the Moon in high enough concentrations to motivate a search mission. During the Late Heavy Bombardment, the inner planets experienced frequent large impacts. Material ejected by these impacts near the escape velocity would have had the potential to land and be preserved on the surface of the Moon. Such ejecta could yield information on the geochemical and biological state of early Earth, Mars, and Venus. To determine whether the Moon has preserved enough ejecta to justify a search mission, we calculate the amount of terran material incident on the Moon over its history by considering the distribution of ejecta launched from the Earth by large impacts. In addition, we make analogous estimates for Mars and Venus. We find, for a well-mixed regolith, that the median surface abundance of terran material is roughly 7 ppm, corresponding to a mass of approximately 20,000 kg of terran material over a 10×10-square-km area. Over the same area, the amount of material transferred from Venus is 1-30 kg and material from Mars as much as 180 kg. Given that the amount of terran material is substantial, we estimate the fraction of this material surviving impact with intact geochemical and biological tracers.     

Le domaine Tariquide (arc de Gibraltar,Espagne et Maroc) : succession sédimentaire et événements structuraux au Lias et au Dogger

The Lias–Dogger successions of the Tariquide units in the Gibraltar Arc differ from the series of tectonically adjacent units, the Penibetic (Iberia margin) and the Limestone ‘Dorsale’ (Alboran Domain). Lower Lias limestones are eroded and covered by the Domerian, which starts by deposits of open sea, continuous up to the Dogger (J. Musa-type successions), and elsewhere (Los Pastores-type successions) by a thin series (Upper Lias to Upper Dogger), starting by a manganesiferous episode (Ras Leona) and including marls deposited into three episodes, dated by calcareous nannofossils. Faulting events occurred before the Domerian and during the Late Toarcian (Los Pastores). To cite this article: M. Durand-Delga et al., C. R. Geoscience 337 (2005).     

Importance of orographic precipitation to the water resources of Monteverde,Costa Rica

Monteverde, Costa Rica harbors montane forests that exemplify the delicate balances among climate, hydrology, habitat, and development. Most of the annual precipitation to this region arrives during the wet season, but the importance of orographic precipitation during the dry and transitional seasons should not be underestimated. Development associated with ecotourism has put significant stress on water resources, and recent work has shown evidence that changes in regional land-cover and global climate may lead to reduced precipitation and cloud cover and a subsequent decline in endemic species.     

Cosmic and Galactic neutrino backgrounds from thermonuclear sources

We estimate energy spectra and fluxes at the Earth’s surface of the cosmic and Galactic neutrino backgrounds produced by thermonuclear reactions in stars. The extra-galactic component is obtained by combining the most recent estimates of the cosmic star formation history and the stellar initial mass function with accurate theoretical predictions of the neutrino yields all over the thermonuclear lifetime of stars of different masses. Models of the structure and evolution of the Milky Way are used to derive maps of the expected flux generated by Galactic sources as a function of sky direction. The predicted neutrino backgrounds depend only slightly on model parameters. In the relevant 50 keV–10 MeV window, the total flux of cosmic neutrinos ranges between 20 and 65 cm⁻² s⁻¹. Neutrinos reaching the Earth today have been typically emitted at redshift z2. Their energy spectrum peaks at E0.1–0.3 MeV. The energy and entropy densities of the cosmic background are negligible with respect to the thermal contribution of relic neutrinos originated in the early universe. In every sky direction, the cosmic background is outnumbered by the Galactic one, whose integrated flux amounts to 300–1000 cm⁻² s⁻¹. The emission from stars in the Galactic disk contributes more than 95% of the signal.     

Do endocrine disrupting chemicals threaten Mediterranean swordfish? Preliminary results of vitellogenin and Zona radiata proteins in Xiphias gladius

Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fishes. In this research the hypothesis that Mediterranean top predator species (such as large pelagic fish) are potentially at risk due to EDCs is investigated. These marine organisms tend to accumulate high concentrations of EDCs such as polyhalogenated aromatic hydrocarbons (PHAHs). The potential effects of EDCs on a fish species of commercial interest, the top predator Xiphias gladius (swordfish), were investigated using vitellogenin (Vtg) and Zona radiata proteins (Zrp) as diagnostic and prognostic biomarkers. Dramatic induction of typically female proteins (Vtg and Zrp) was detected by ELISA and Western Blot in adult males of the species. These results are the first warning of the potential risk for reproductive function of Mediterranean top predators, and suggest the need for continuous monitoring of this fragile marine environment.     

Geomorphological significance of Ontario Lacus on Titan: Integrated interpretation of Cassini VIMS,ISS and RADAR data and comparison with the Etosha Pan (Namibia)

Ontario Lacus is the largest lake of the whole southern hemisphere of Titan, Saturn’s major moon. It has been imaged twice by each of the Cassini imaging systems (Imaging Science Subsystem (ISS) in 2004 and 2005, Visual and Infrared Mapping Spectrometer (VIMS) in 2007 and 2009 and RADAR in 2009 and 2010). We compile a geomorphological map and derive a “hydrogeological” interpretation of Ontario Lacus, based on a joint analysis of ISS, VIMS and RADAR SAR datasets, along with the T49 altimetric profile acquired in December 2008. The morphologies observed on Ontario Lacus are compared to landforms of a semi-arid terrestrial analog, which resembles Titan’s lakes: the Etosha Pan, located in the Owambo Basin (Namibia). The Etosha Pan is a flat-floored depression formed by dissolution, under semi-arid conditions, of a surface evaporitic layer (calcretes) controlled by groundwater vertical motions. We infer that Ontario Lacus is an extremely flat and shallow depression lying in an alluvial plain surrounded by small mountain ranges under climatic conditions similar to those of terrestrial semi-arid regions. Channels are seen in the southern part of Ontario Lacus in VIMS and RADAR data, acquired at a 2-years time interval. Their constancy in location with time implies that the southern portion of the depression is probably not fully covered by a liquid layer at the time of the observations, and that they most probably run on the floor of the depression. A shallow layer of surface liquids, corresponding to the darkest portions of the RADAR images, would thus cover about 53% of the surface area of the depression, of which almost 70% is located in its northern part. These liquid-covered parts of the depression, where liquid ethane was previously identified, are interpreted as topographic lows where the “alkanofer” raises above the depression floor. The rest of the depression, and mostly its southern part, is interpreted as a flat and smooth exposed floor, likely composed of a thick and liquid-saturated coating of photon-absorbing materials in the infrared. This hypothesis could explain its dark appearance both in the infrared and radar data and the persistence of channels seen on the depression floor over the time. Shorelines are observed on the border of Ontario Lacus suggesting past high-stand levels of the alkanofer table. The analogy with the Etosha Pan suggests that Ontario Lacus’ depression developed at the expense of a soluble layer covering the region. Dissolution of this layer would be controlled by vertical motions of the alkanofer table over the time. During flooding events, liquid hydrocarbons covering the depression floor would dissolve the surface layer, increasing progressively the diameter of the depression on geological timescales. During drought episodes, liquid hydrocarbons of the underground alkanofer would evaporate, leading to crystallization of “evaporites” in the pores and at the surface of the substratum, and to the formation of the regional soluble layer. The presence of specific landforms (lunette dunes or evaporites) is compatible with such evaporitic regional settings. Alternatively, but not exclusively, the surface soluble layer might have formed by accumulation on the ground of soluble compounds formed in the atmosphere.     

Coronal Density Structures and CMEs: Superior Solar Conjunctions of Mars Express, Venus Express, and Rosetta: 2004, 2006, and 2008

Coronal radio-sounding experiments were carried out using the S-band (2.3 GHz) and X-band (8.4 GHz) signals of the ESA Mars Express, Venus Express, and Rosetta spacecraft during five superior conjunctions occurring in 2004, 2006 (3×), and 2008/2009. Differential frequency and propagation delay (ranging) observations were recorded during these opportunities over the better part of a solar cycle, yielding information on the large-scale structure of the coronal electron-density distribution and its variations, including fluctuations on time scales from seconds to hours. These results concern primarily regions of slow solar wind because the radio propagation path is generally confined to the low heliolatitude regions by the conjunction. The mean frequency fluctuation and total electron content are determined as a function of heliocentric distance, and, with a few exceptions caused by streamers and CMEs, are found to be consistent with previous results from experiments on Ulysses. Dense coronal streamers and several coronal mass ejection (CME) events were identified in the radio-frequency data, some of which were observed in white light by the LASCO coronagraphs onboard SOHO. For those events with sufficient mutual coverage, good correlations are found between the electron-content fluctuations and structure imaged by the LASCO instrument.     

Influence of the properties of tropical soils in the toxicity and bioavailability of heavy metals in sewage sludge-amended lands

The toxicity and potential bioavailability of heavy metals were studied in sewage sludge-amended ferralsols and chernosols, using bioassays with earthworms (Eisenia andrei). Acute toxicity tests and avoidance assays were performed according to standard protocols. Potentially bioavailable concentrations of heavy metals were estimated by quantifying their concentrations in tissues of survival earthworms. The results revealed that soil properties played an important role in the toxicity and bioavailability of heavy metals. In this respect, higher levels of toxicity were detected for the ferralsol. The abundance of 2:1 clay minerals, high fertility and more basic values of pH seem to be very important in the reduction of toxicity levels for earthworms. Organisms exposed to the chernosols were able to uptake higher amount of metals. In that case, higher contents of nutrients in chernosols may have influenced such bioavailability processes. Avoidance responses were the most sensible indicator of toxicity. In the near future, such data can subsidize the establishment of toxic reference concentrations able to reflect the characteristics of important tropical pedological occurrences, supporting the definition of sustainable indicators for using sewage sludge in the tropical agriculture.