多测绘技术手段与遗址保护

采用全站仪离散点测量、三维激光扫描、无人机航拍对大型古遗址进行数据采集,分析了各种测绘手段的作业方法及流程,得到一系列测绘成果,并利用三维浏览软件实现遗址的三维展示,为遗址修缮与保护提供了数据支持。     

通过软硬变化检测识别冬小麦

提出一种软硬变化检测的作物识别方法 SHLUCD(Soft and Hard Land Use/Cover Change Detection Method)。该方法利用多期遥感影像能够有效表达作物的生长物候特征,以达到在离散变化区(即纯净像元区,包括完全转换成作物的突变区域和非作物区域)和连续变化区(即渐变区,混合像元区,是部分转化为作物的区域)准确进行作物的识别。在北京市选择一个研究区,以冬小麦为研究对象,选用2011年10月6日(播种期)和2012年4月16日(拔节期)两期环境减灾1号卫星影像,分别采用硬变化检测方法 HLUCD(Hard Land Use/Cover Change Detection Method)、软变化检测方法 SLUCD(Soft Land Use/Cover Change Detection Method)和SHLUCD进行冬小麦的识别。实验结果表明:在不同尺度窗口下,SHLUCD较传统方法表现出较明显的优势,具有更低的均方根误差RMSE(SHLUCD为[0.14,0.07],HLUCD为[0.15,0.07],SLUCD为[0.16,0.08])和偏差bias(SHLUCD为-0.0008,HLUCD为-0.007,SLUCD为0.014)和更高的决定系数R2(SHLUCD为[0.68,0.86],HLUCD为[0.62,0.86],SLUCD为[0.60,0.86])。针对冬小麦突变区域、冬小麦渐变区域和非冬小麦区域分别进行评价,表明SHLUCD识别精度接近各区最佳的识别方法,进一步验证了SHLUCD的灵活性和适用性。SHLUCD方法在离散变化区能够通过土地覆盖类型状态变化来有效地识别出冬小麦,在连续变化区可识别出土地覆盖的状态变化程度定量表达冬小麦的丰度,是其他作物多时相遥感变化检测的前期实验基础。     

基于GIS的西平县土地利用变化分析

在遥感和地理信息系统软件支持下,通过2002年的ETM＋和2007年的CBERS遥感图像分析,利用马尔科夫链的元胞自动机模型与构建土地利用适宜性图层,模拟西平县2012年土地利用变化。结果表明：到2012年,西平县城镇及工矿用地将在原有基础上向四周辐射发展,交通运输用地将在原来基础上拓宽,村镇居民点及公路周围的耕地将减少,林地、其他用地也将减少。     

Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a marine ciliate, Pleuronema coronatum (ciliophora, scuticociliatida)

Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations (LC₅₀ values) were given. Results of measurements indicated that 2 h-LC₅₀ and 12 h-LC₅₀ values of formalin on P. coronatum were 59.00×10⁻⁶ and 43.57×10⁻⁶, while those of Lugol’s solutions were 90.13 and 67.84×10⁻⁶ respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to kill ciliates in short time.     

论多种数据联合反演的模式及算法

针对日益丰富的数据资料，综述了大地测量反演的发展进程，论述了多种数据联合反演的意义和必要性，给出了兼顾权比确定的大地测量、地震和地质三类数据的联合反演模式及采用水准测量、GPS和重力三种数据联合反演断层参数的具体公式，特别介绍了求解优化问题所有全局最优解的区间算法，并给出了基本算例，结果体现了区间算法无可比拟的优越性及其在大地测量反演领域中的应用前景。     

Control factors of DIC in the Y3 seamount waters of the Western Pacific Ocean

An investigation was carried out in the Y3 seamount area of the Western Pacific Ocean in December 2014,and the distribution of dissolved inorganic carbon(DIC) and its relationship with environmental factors in this area were explored.The results show that DIC concentration was higher in the adjacent waters of the Y3 seamount area,and the uplift of DIC isolines at the stations was close to the seamount.Meanwhile,interaction between the North Equatorial Current(NEC) and the Y3 seamount affected the DIC distribution i.e.,the upwelling in the same direction of the NEC was obvious,resulting in a decreasing trend of average concentration of DIC in the 200 m water column from the top to the two side s in this direction but in the cross direction.The DIC concentration increased with the water depth increase,and its distribution was affected by various environmental factors.In the surface water,high temperature was a decisive factor for the decrease of the DIC concentration,but the photosynthesis of phytoplankton showing only a weak influence.In the North Pacific Tropic Water(NPTW),DIC production rate from organic matter decomposition was higher than that of DIC consumption by phytoplankton photosynthesis,leading to a continual increase of DIC.In the North Pacific Intermediate Water(NPIW),organic matter decomposition played a leading role in the increase of DIC.In the deep water,decomposition of organic matter weakened,and the dissolution of CaCO_3 controlled the carbonate system,and DIC had the smallest variation range.     

合浦珠母贝丝氨酸蛋白酶抑制因子基因pfser1克隆与表达

【目的】克隆合浦珠母贝(Pinctada fucata)丝氨酸蛋白酶抑制因子pfser1基因,探讨该基因的组织表达及其在天然免疫过程中的作用,以及与生物矿化过程的关系。【方法】通过RACE技术获得pfser1基因的全长,通过生物信息学分析其序列结构特征,利用实时荧光定量PCR方法检测pfser1基因在不同组织中的表达,检测健康合浦珠母贝在被大肠杆菌(Escherichia coli)MG1655刺激后和在贝壳损伤修复实验中pfser1基因表达量的变化。【结果】合浦珠母贝pfser1基因cDNA全长为1240 bp,包含1035 bp的开放阅读框(ORF),编码344个氨基酸,氨基酸序列的功能结构域含有丝氨酸蛋白酶抑制因子Serpin家族保守结构域。pfser1基因在合浦珠母贝各个组织中均有表达,在外套膜边缘膜中表达量最高;大肠杆菌MG1655刺激后,该基因表达量显著升高;在贝壳损伤修复过程中,pfser1基因表达先升高后受到抑制。【结论】pfser1基因所表达的蛋白参与了合浦珠母贝的天然免疫应答过程,并与生物矿化过程有一定关系。     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

Dynamics and relationships of Ca, Mg, Fe in litter, soil fauna and soil in Pinus koraiensis-broadleaf mixed forest

The Liangshui Natural Reserve in Heilongjiang Province of China was selected as the study area. The authors collected the samples of forest litter （Tilia amurensis, Fraxinus mandshurica, Pinus koraiensis, Acer mono, Betula costata, and mixed litter）, soil in humus horizon （0--5cm） and soil horizon （5-20cm）, and soil macrofauna （Oligochaeta, Geophiloporpha and Juliformia） from 2001 to 2002. The role of soil macrofauna in the material cycle was analyzed through comparing the macro-element contents among various parts of the subsystems and using enrichment index （El）. The results indicate that dynamic changes of various litters are very complicated. The contents of Fe in each kind of litter increase firstly, and then decrease in the study period. The changes of macro-element contents are greater in the broad-leaf litter than in the coniferous litter, and the mixed litter is in the middle level, but the differences among them are not significant. The contents of Mg and Fe in humus are higher than those in soil, but the contents of Ca in soil are higher than that in humus. The dynamic changes of macro-element contents in soil and soil fauna are not consistent with those in litter. The diplopod presented obvious enrichment of Ca and Mg （E1〉1）, but it does not significantly enrich Fe. Earthworm has a stronger enrichment ability of Fe than diplopod and scolopendra, but E1〈1. Soil fauna can make great influences on the material cycle of the subsystems.     

Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice

Excessive alcohol consumption leads to liver disease. Extensive evidence suggests that C-phycocyanin (C-PC), a chromophore phycocyanobilin derived from Spirulina platensis, exerts protective effects against chemical-induced organ damage. In this study, we investigated whether C-PC could protect against ethanol-induced acute liver injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHOL), low-density lipoprotein (LDL), liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD) content were measured, and pathological examination of liver sections were examined. C-PC showed obvious inhibitory effects on serum ALT, AST, TG, CHOL, LDL and MDA, and SOD content significantly increased in the liver. The structure of hepatic lobules was clear, liver sinus returned to normal, and liver cell cords were arranged in neat rows. Cloudiness, swelling, inflammatory cell infiltration and spotty necrosis of liver cells were significantly reduced. Therefore, C-PC can significantly protect against ethanol-induced acute liver injury.