Opposite diel patterns of nitrogen fixation associated with salt marsh plant species (Spartina foliosa and Salicornia virginica) in southern California

In marine wetlands, nitrogen fixation is a potentially important nutrient source for nitrogen‐limited primary producers, but interactions between nitrogen fixers and different vascular plant species are not fully understood. Nitrogen fixation activity was compared in sediments vegetated by three plant species, Spartina foliosa, Salicornia virginica, and Salicornia bigelovii in the Kendall Frost Reserve salt marsh in Mission Bay (CA). This study addressed the effects of plant type, day and night conditions, and sediment depths on nitrogen fixation. Higher rates of nitrogen fixation were associated with S. foliosa than with either of the two Salicornia spp., which are known to compete more effectively than Spartina for exogenous nitrogen in the salt marsh environment. Rates of nitrogen fixation, determined by acetylene reduction, in sediments vegetated by S. virginica were low during the day (7.7 ± 1.2 μmol C₂H₄ m⁻² h⁻¹) but averaged 13 ± 6.6 μmol C₂H₄ m⁻² h⁻¹ at night, with particularly high rates in samples from locations with visible cyanobacterial mats. The opposite diel pattern was found for sediments containing S. foliosa plants, in which average daytime and nighttime rates of nitrogen fixation were 62 ± 23 and 21 ± 15 μmol C₂H₄ m⁻² h⁻¹, respectively. For S. foliosa, nitrogenase activity of rinsed roots and different sediment sections (0–1, or 4–5 cm depths) were measured. Although nitrogen fixation rates in vegetated sediment samples were substantial, all but one of rinsed S. foliosa root samples (n = 12) and subsurface sediments at 4–5 cm depths failed to show nitrogen fixation activity after 2 h, suggesting that the most active nitrogen fixers in these systems likely reside in surface sediments. Further, nitrogenase activity in shaded and unshaded S. foliosa samples did not differ, suggesting that nitrogen fixers may not rapidly respond to changes in plant photosynthetic activity. Average nitrogen fixation rates in S. foliosa‐vegetated samples from the Mission Bay salt marsh were on the same order as those of highly productive Atlantic coast marshes, and this microbially‐mediated nitrogen source may be similarly substantial in other Mediterranean wetlands. Sediment abiotic variables seem to exert greater control upon nitrogen fixation activity than the effects of particular plant species. Nonetheless, dominant plant species may differ substantially in their reliance on nitrogen fixation as a nutrient source, with potentially important consequences for wetland conservation and restoration.     

Reconstruction of the trajectory of the Huygens probe using the Huygens Atmospheric Structure Instrument (HASI)

The Huygens probe returned scientific measurements from the atmosphere and surface of Titan on 14 January 2005. Knowledge of the trajectory of Huygens is necessary for scientific analysis of those measurements. We use measurements from the Huygens Atmospheric Structure Instrument (HASI) to reconstruct the trajectory of Huygens during its mission. The HASI Accelerometer subsystem measured the axial acceleration of the probe with errors of 3E−6 m s⁻², the most accurate measurements ever made by an atmospheric structure instrument on another planetary body. The atmosphere was detected at an altitude of 1498 km. Measurements of the normal acceleration of the probe, which are important for determining the probe's attitude during hypersonic entry, were significantly less accurate and limited by transverse sensitivity of the piezo sensors. Peak acceleration of 121.2 m s⁻² occurred at 234.9 km altitude. The parachute deployment sequence started at 157.1 km and a speed of 342.1 m s⁻¹. Direct measurements of pressure and temperature began shortly afterwards. The measured accelerations and equations of motion have been used to reconstruct the trajectory prior to parachute deployment. Measured pressures and temperatures, together with the equation of hydrostatic equilibrium and the equation of state, have been used to reconstruct the trajectory after parachute deployment. Uncertainties in the entry state of Huygens at the top of the atmosphere are significant, but can be reduced by requiring that the trajectory and atmospheric properties be continuous at parachute deployment.     

Huygens probe entry dynamic model and accelerometer data analysis

During the first phase of Huygens arrival into Titan's atmosphere the probe is subjected to gravitational and aerodynamic forces in aerodynamic hypersonic regime. Atmospheric drag exerts a strong deceleration on the capsule measured by Huygens atmospheric structure instrument (HASI) servo accelerometer. A 6 DOF (Degree of Freedom) model of the Huygens probe entry dynamics has been developed and used for data analysis. The accelerometer data are analysed and the model allows the retrieval of dynamics information of Huygens probe from 1545 km altitude down to end of the entry phase. Probe's initial conditions (velocity and position) were refined to match the measured deceleration profile resulting in a different altitude at interface epoch with respect to those of the Cassini Navigation Team. Velocity and position of probe at interface epoch are compatible with those used by Descent Trajectory Working Group (DTWG).Measurements acquired before atmosphere detection are used to estimate probe's angular rate, bound attitude and characterise the angle of attack profile which results to be lower than 4^° during the whole entry. Probe's spin calculated (6.98 RPM) is slightly different with respect to DTWG of 7.28 RPM but considering a 2% error in the Inertia matrix these results are inside the 1-σ error band.     

Double origin of hydrothermal convective flux variations in the Fossa of Vulcano (Italy)

Soil-temperature measurements can provide information on the distribution of degassing fissures, their relationship to the internal structure of the volcano, and the temporal evolution of the system. At Vulcano Island (Italy), heat flux from a <3 km-deep magma body drives a hydrothermal system which extends across the main Fossa crater. This heat flux is also associated with variable magmatic gas flow. A high-density map of soil-temperatures was made in 1996 at a constant depth of 30 cm on the central and southern inner flanks of the Fossa crater. These measurements extended over an area covering about 0.04 km², across which the heat flux is predominantly associated with a shallow boiling aquifer. The map shows that hot zones relate to structures of higher permeability, mainly associated with a fissure system dating from the last eruptive cycle (1888–1890). From 1996 to January 2005, we studied the evolution of the heat flux for the high temperature part of the map, both by repeating our measurements as part of 14 visits, during which temperatures were measured at a constant depth, and using data from permanent stations which allowed soil-temperatures to be continuously measured for selected vertical profiles. These data allowed us to calculate the heat flux, and its variation, with good precision for values lower than about 100 W m⁻², which is generally the case in the study area. Above 100 W m⁻², although the heat flux value is underestimated, its variations are recorded with an error less than 10%. During the period 1996–2004, two increases in the thermal flux were recorded. The first one was related to the seismic crisis of November 1998 which opened existing or new fissures. The second, in November 2004, was probably due to magma migration, and was associated with minor seismic activity.     

Effects of offshore platforms on soft-bottom macro-benthic assemblages: a case study in a Mediterranean gas field

The exploitation of fossil fuels in the Mediterranean Sea will likely lead to an increase in the number of offshore platforms, a recognized threat for marine biodiversity. To date, in this basin, few attempts have been made to assess the impact of offshore gas and oil platforms on the biodiversity of benthic assemblages. Here, we adopted a structured experimental design coupled with high taxonomic resolution to outline putative effects of gas platforms on soft-bottom macrofauna assemblages in the North Ionian Sea. The analysis was based on a total of 20,295 specimens of 405 taxa, almost entirely identified at species level. Multivariate and univariate analyses showed idiosyncratic patterns of assemblage change with increasing distance from the platforms. Potential reasons underlying such inconsistency are analyzed and the view that structured experimental monitoring is a crucial tool to quantify the extent and magnitude of potential threats and to provide sound baseline information on biodiversity patterns is supported.     

Diversity and functions of microscopic fungi: a missing component in pelagic food webs

Fungi are a highly complex group of organisms of the kingdom Eumycota (i.e. the true-fungi) and other fungus-like organisms traditionally studied by mycologists, such as slime molds (Myxomycota) and oomycota (Straminopiles or Heterokonts). They constitute a significant proportion of the as yet undiscovered biota that is crucial in ecological processes and human well-being, through at least three main trophic modes: saprophytism, parasitism, or symbiosis. In addition to direct benefit (sources of antibiotics) or adverse effects (agents of disease), fungi can impact many environmental processes, particularly those associated with the decomposition of organic matter. They are present in almost all regions and climates, even under extreme conditions. However, studies have focussed mostly on economically interesting species, and knowledge of their diversity and functions is mainly restricted to soil, rhizosphere, mangrove, and lotic ecosystems. In this study, we review the diversity and potential functions of microscopic fungi in aquatic ecosystems, with focus on the pelagic environments where they often are regarded as allochthonous material, of low ecological significance for food-web processes. Recent environmental 18S rDNA surveys of microbial eukaryotes have (1) unveiled a large reservoir of unexpected fungal diversity in pelagic systems, (2) emphasized their ecological potentials for ecosystem functioning, and (3) opened new perspectives in the context of food-web dynamics. In spite of persisting methodological difficulties, we conclude that a better documentation of the diversity and quantitative and functional importance of fungi will improve our understanding of pelagic processes and biogeochemical cycling.     

The roots of Mt. Vesuvius deduced from gravity anomalies

Summary The structures of the Somma-Vesuvius volcanic complex are modelled on the basis of the interpretation of gravity anomalies obtained from data available in the literature and acquired along a new profile along the coastline from Naples to Castellammare di Stabia. In order to highlight the contribution of shallow crustal structures, the residual anomalies were considered. A marked gravity low was recognised in the eastern sector of Vesuvius. Furthermore data interpretation was carried out along two profiles centred on the low gravity region in question: a first profile crossing the Vesuvius crater in direction WNW-ESE, and a second one in NNE-SSW direction. The 2 ? D model obtained reveals a crustal structure characterised by sediments of 2.3 Mg/m³ density, overlying bedrock with a density of 2.6 Mg/m³. Near the volcanic system the model becomes more complex due to the presence of light sediments with a density of 2.1 Mg/m³ overlying a body with a density of 2.4 Mg/m³ which extends into depth. The latter is thought to be closely related to the zone of magma ascent developed along the volcanic axis. Along the coast the volcanic component is reduced and the model shows that the layer with a density of 2.3 Mg/m³ ranges in thickness from 0 to about 3500 m. An additional body between 1500 and 3000 m with a density of 2.4 Mg/m³ was considered in order to account for the slight rise in the residual anomaly in the area in the vicinity of Mt. Vesuvius. The analysis of the gravity anomaly pattern coincides with the complex system of faults and fractures intersecting the carbonate basement and the volcanic area in question, which developed as a consequence of extensional processes at the continental edge of the Italian Peninsula due to the opening of the Tyrrhenian basin. This extensional tectonics has created favourable conditions for the collapse of the south-western slope of Mt. Vesuvius and the development of eruptive vents and cracks on its flanks. Received May 18, 2000; revised version accepted March 6, 2001     

Carbon monoxide in low-mass dwarf stars

We compare high-resolution infrared observations of the CO 2–0 bands in the 2.297–2.310 μm region of M dwarfs and one L dwarf with theoretical expectations. We find a good match between the observational and synthetic spectra throughout the 2000–3500 K temperature regime investigated. None the less, for the 2500–3500 K temperature range, the temperatures that we derive from synthetic spectral fits are higher than expected from more empirical methods by several hundred kelvin. In order to reconcile our findings with the empirical temperature scale, it is necessary to invoke warming of the model atmosphere used to construct the synthetic spectra. We consider that the most likely reason for the back-warming is missing high-temperature opacity due to water vapour. We compare the water vapour opacity of the Partridge–Schwenke line list used for the model atmosphere with the output from a preliminary calculation by Barber & Tennyson. While the Partridge–Schwenke line list is a reasonable spectroscopic match for the new line list at 2000 K, by 4000 K it is missing around 25 per cent of the water vapour opacity. We thus consider that the offset between empirical and synthetic temperature scales is explained by the lack of hot water vapour used for computation of the synthetic spectra. For our coolest objects with temperatures below 2500 K, we find best fits when using synthetic spectra which include dust emission. Our spectra also allow us to constrain the rotational velocities of our sources, and these velocities are consistent with the broad trend of rotational velocities increasing from M to L.     

Huygens/HASI 2002 balloon test campaign: Probe trajectory and atmospheric vertical profiles reconstruction

In the framework of the activities going on in preparation for the mission of the Huygens probe in Titan's atmosphere (January 2005), the Huygens Atmospheric Structure Instrument (HASI) team scheduled and performed several balloon campaigns to test the HASI sensors’ performance in flight conditions in the Earth's atmosphere. In particular, pressure conditions reached during each test are similar to those expected in Titan's lower atmosphere. A 1:1 scaled mock-up of the Huygens probe was launched with a stratospheric balloon in 2001 (Br. Assoc. Adv. Sci. 33 (2001) 1109) and in 2002 (Br. Assoc. Adv. Sci. 34 (2002) 911; Adv. Space. Sci. (2003)) from the G. Broglio base of the Italian Space Agency, located in Trapani Milo (Sicily). In both cases the mock-up was dropped from an altitude higher than 27 and , respectively, and recovered on the ground after a parachuted descent. In this paper, we describe the results obtained in reconstructing (i) the probe descent trajectory and (ii) the profiles of the physical quantities characterizing the Earth's atmosphere, on the basis of a complete analysis of the data obtained during the HASI 2002 balloon flight experiment. Using temperature and pressure measurements, we are able to reach an accuracy of the order of 0.5% on the altitude reconstruction during the descent. We validate both the models used for trajectory reconstruction and to check the sensors’ performance. We describe the problems faced in determining the Huygens probe descent trajectory in Titan's atmosphere focusing our discussion on the critical aspects of the descent reconstruction (such as the uncertainties due to measurement errors, limited knowledge of the atmospheric composition, etc.) and the validity of the adopted assumptions.