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101.
β-mannanase is an enzyme that is commonly expressed in environmental bacteria. It degrades hemicellulose found in plant material and recycles nutrients back into the environment. Because this enzyme significantly contributes to biodegradation and has recently been applied in industry, we conducted a comparative analysis of bacterial isolates found in soil samples from Schirmacher Oasis, Antarctica, and Sabah, Malaysia that were capable of degrading mannan. A total of 9 bacterial isolates from Antarctica and 30 bacterial isolates from Malaysia exhibited β-mannanase activity. These bacteria were differentiated and clustered using their random amplified polymorphic DNA (RAPD) profiles, and the β-mannanase activity of these isolates was tested at different temperatures and pH. Five out of 9 Antarctica isolates and seven out of 30 Malaysian isolates were identified based on their 16S rDNA sequences. Identified bacterial isolates from Antarctica were: MP1 (Bacillus amyloliquefaciens), MP2 (Bacillus pumilus), MP5 (Bacilluspumilus), A40 (Arthrobacter sp.), and C27 (Arthrobacter oxydans). Identified bacterial isolates from Ma- laysia were: Y1 (Paenibacillus sp.), Y2 (Bacillus sp.), Y16 (Paenibacillus sp.), Y18 (Paenibacillus sp.), A7 (Paenibacillus sp.), B26 (Streptomyces sp.), and D4 (Paenibacillus amylolyticus). β-mannanases produced by the Antarctica bacterial isolates MP1 (Bacillus amyloliquefaciens) and A40 (Arthrobacter sp.) were active at 5℃ and 20℃, respectively, while the β-mannanase pro- duced by the bacterial isolate from Malaysia, A7 (Paenibacillus sp.), was active at 35 ℃. 相似文献
102.
Patricia?A.?TesterEmail author Sabrina?M.?Varnam Mary?E.?Culver David?L.?Eslinger Richard?P.?Stumpf Robert?N.?Swift James?K.?Yungel Megan?D.?Black R.?Wayne?Litaker 《Estuaries and Coasts》2003,26(5):1353-1364
Airborne laser-induced fluorescence measurements were used to detect and monitor ecosystem wide changes in the distribution
and concentration of chlorophyll biomass and colored dissolved organic matter in the Pamlico-Albemarle Sound system, North
Carolina, U.S., following massive flooding caused by a series of three hurricanes in the late summer of 1999. These high-resolution
data provided a significantly more detailed representation of the overall changes occurring in the system than could have
been achieved by synoptic sampling from any other platform. The response time for the distribution of chlorophyll biomass
to resume pre-flood conditions was used as a measure of ecosystem stability. Chlorophyll biomass patterns were reestablished
within four mo of the flooding, whereas higher chlorophylla biomass concentrations persisted for approximately 6 mo. The primary trophic level in the Pamlico-Albemarle Sound system
returned to equilibrium in less than a year of a major perturbation. 相似文献
103.
The REMO Ocean Data Assimilation System into HYCOM (RODAS_H): General Description and Preliminary Results
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Clemente Augusto Souza TANAJURA ;Alex Novaes SANTANA ;Davi MIGNAC ;Leonardo Nascimento LIMA ;Konstantin BELYAEV ;XIE Ji-Ping 《大气和海洋科学快报》2014,(5):464-470
The first version of the Brazilian Oceano- graphic Modeling and Observation Network (REMO) ocean data assimilation system into the Hybrid Coordi- nate Ocean Model (HYCOM) (RODAS H) has recently been constructed for research and operational purposes. The system is based on a multivariate Ensemble Optimal Interpolation (EnOI) scheme and considers the high fre- quency variability of the model error co-variance matrix. The EnOl can assimilate sea surface temperature (SST), satellite along-track and gridded sea level anomalies (SLA), and vertical profiles of temperature (T) and salinity (S) from Argo. The first observing system experiment was carried out over the Atlantic Ocean (78°S-50°N, 100°W-20°E) with HYCOM forced with atmospheric reanalysis from 1 January to 30 June 2010. Five integra- tions were performed, including the control run without assimilation. In the other four, different observations were assimilated: SST only (A SST); Argo T-S profiles only (AArgo); along-track SLA only (A_SLA); and all data employed in the previous runs (A_All). The A_SST, A_Argo, and A_SLA runs were very effective in improv- ing the representation of the assimilated variables, but they had relatively little impact on the variables that were not assimilated. In particular, only the assimilation of S was able to reduce the deviation of S with respect to ob- servations. Overall, the A_All run produced a good analy- sis by reducing the deviation of SST, T, and S with respect to the control run by 39%, 18%, and 30%, respectively, and by increasing the correlation of SLA by 81%. 相似文献
104.
Alan J. Lewitus David L. White Raphael G. Tymowski Mark E. Geesey Sabrina N. Hymel Peter A. Noble 《Estuaries and Coasts》2005,28(1):160-172
CHEMTAX is a matrix factorization program used to derive taxonomic structure of phytoplankton from photosynthetic pigment
vitios. The program was originally developed from and applied to the analysis of oceanic phytoplankton assemblages. We found
that application of the original CHEMTAX reference matrix to southeastern United States estuarine systems produced inaccurate
results, as verified by microscopy. Modification of the matrix, based primarily on the pigment ratios of 33 estuarine isolates,
improved the predictive capabilities of CHEMTAX for our samples. Limitations of the method included an overstimation of diatom
biomass (due to the inability to differentiate diatoms from taxa with chloroplasts derived from diatom endosymbionts, notably
some dinoflagellates) and a tendency to exclude some raphidophyte species. In complement with microscopic verification, the
method was shown to improve assessment of phytoplankton taxonomic composition. 相似文献
105.
106.
Many clay rocks have distinct bedding planes. Experimental studies have shown that their mechanical properties evolve with the degree of saturation (DOS), often with higher stiffness and strength after drying. For transversely isotropic rocks, the effects of saturation can differ between the bed-normal (BN) and bed-parallel (BP) directions, which gives rise to saturation-dependent stiffness and strength anisotropy. Accurate prediction of the mechanical behavior of clay rocks under partially saturated conditions requires numerical models that can capture the evolving elastic and plastic anisotropy with DOS. In this study, we present an anisotropy framework for coupled solid deformation-fluid flow in unsaturated elastoplastic media. We incorporate saturation-dependent strength anisotropy into an anisotropic modified Cam-Clay (MCC) model and consider the evolving anisotropy in both the elastic and plastic responses. The model was calibrated using experimental data from triaxial tests to demonstrate its capability in capturing strength anisotropy at various levels of saturation. Through numerical simulations, we demonstrate the role of evolving stiffness and strength anisotropy in the mechanical behavior of clay rocks. Plane strain simulations of triaxial compression tests were also conducted to demonstrate the impacts of material anisotropy and DOS on the mechanical and fluid flow responses. 相似文献
107.
Camila Rodrigues Costa Cynthia Fernandes Pinto da Luz Ingrid Horák-Terra Plínio Barbosa de Camargo Uidemar Morais Barral Carlos Victor Mendonça-Filho Thamyres Sabrina Gonçalves Alexandre Christófaro Silva 《第四纪科学杂志》2023,38(1):61-75
The Cerrado biome is the second largest in Brazil, but the evolution of the Cerrado during the late Quaternary is not yet fully known. This study identifies paleoenvironmental changes during the last 23 000 years, based on a tropical mountain peatland record, in the Serra do Espinhaço Meridional in central-eastern Brazil. A multi-proxy approach was used that involved palynological analysis, stable isotopes (δ13C, δ15N), geochemistry, radiocarbon dating and multivariate statistics derived from a peatland core from Rio Preto (Minas Gerais state). The study reveals a very humid and cold climate during the late Pleistocene, with an increase in temperature and decrease in humidity at the Pleistocene–Holocene transition. During this period there was strong instability in the landscape (episodes of erosion). At the beginning of the Holocene there was a reduction in humidity with greater landscape stability. The current sub-humid climatic conditions seem to have been established in the mid-/late Holocene, with periods of landscape instability. Our findings agree with other Cerrado records that contradict previously established hypotheses, such as the Amazonian Refuge and the Pleistocene Arc. 相似文献