Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

Relationships between body weight and other morphological traits in young sea cucumbers Apostichopus japonicas

Correlation, multiple regression, and path analyses were used to investigate the relationships between body weight and three other morphological traits in juvenile Japanese sea cucumbers Apostichopus japonicus . We measured live body weight (BW), body length (BL), numbers of papillae (NP), and numbers of tube feet (NF) at 60, 80, 100, and 130 days post-hatching (dph). We calculated path correlation coefficients, correlation indices ( R 2 ), and coefficients of determination with BW as the dependent variable and the other morphological traits as independent variables. The coefficient of variation for BW was high across all age groups, and all measured morphological traits were significantly correlated ( P < 0.01). BL had the greatest direct eff ect on BW across all age groups (60 dph, 0.526;80 dph, 0.404;100 dph, 0.620;and 130 dph, 0.681), while NF had the greatest indirect eff ect on BW across all age groups (60 dph, 0.528;80 dph, 0.452;100 dph, 0.666;and 130 dph, 0.603). Regression analyses between morphological traits and BW indicated that R 2 was greater than 0.85 only in the 100-dph specimens. The indirect eff ects of the other measured morphological traits on BW were age-dependent. The optimal regression equations, as determined with stepwise regression, were, for 60-dph specimens: BW 60 =10 (-3.04+0.092BL+0.014NP+0.014NF)( R 2 =0.632);for 80-dph specimens: BW 80 =10 (- 3.035+0.056BL+0.017NP+0.02NF)( R 2 =0.686);for 100-dph specimens: BW 100 =10 (- 3.742+0.069BL+0. 633 · lg (NP)+0. 464 · lg (NF))( R 2 =0.893);and for 130-dph specimens: BW 130 =10 (- 2.472+0.065BL+0.012NP)( R 2 =0.774). Our work clarified the correlation between various morphological traits and body weight of a commercially-important sea cucumber species ( A . japonicus ). Our predictive models for body weight might be useful for the aquaculture and selective breeding of A . japonicus . These models might also provide theoretical support for the indirect selection of traits that are difficult to select directly.     

Comparative study of otolith and sulcus morphology for stock discrimination of yellow drum along the Chinese coast

Otolith morphology is widely used for fish stock identification. The sulcus, a structure on the medial side of the otolith, is an important feature in morphological analysis. This study was conducted to evaluate the feasibility of using sulcus morphology for stock identification and to compare its performance with commonly used otolith morphology analysis. Otoliths were collected and analyzed from three geographical groups (the Huanghe (Yellow) River estuary, HHE;the Jiaozhou Bay, JZB;and the Changjiang (Yangtze) River estuary, CJE) of yellow drum Nibea albiflora. The results show that the analysis of sulcus morphology based on shape indices (SIs), elliptic Fourier coefficients (EFc), and a combination of the two parameters identified stocks at overall classification rates of 51.0%, 72.5%, and 73.2%, respectively. These classification rates are similar to those obtained using otolith morphology analysis (57.0%, 73.8%, and 76.5% by SIs, EFc, and their combination, respectively). The findings suggest that sulcus morphology is comparable to the commonly used otolith morphology for identifying stocks of sciaenids, such as the yellow drum. For both otolith and sulcus morphology, EFc could identify the stocks more efficiently than SIs, while the combination of SIs and EFc was even better.     

基于椭球不确定性的平差模型与算法

测量平差模型中的参数通常存在一些不确定的附加信息或先验信息,充分利用它们可以对部分参数进行约束,从而保证参数解的唯一性和稳定性。本文利用椭球集合描述不确定性,建立了一个新的带有椭球不确定性的平差模型。以两个椭球交集的外接椭球的特征矩阵的迹最小平差准则,分析了不确定度的传播规律,给出了带有椭球不确定性的平差方法。最后,通过算例验证了算法的有效性,说明了平差解与带权混合估计的关系。     

地灾监测的大数据信息平台研究及应用

重点对地灾监测方面的方案研究进行了阐述,在构建大数据信息平台的设计方案时,以实现高度自动化的全方位监测功能为导向,依托导航卫星数据接收机和多源传感器,集成构建大数据信息平台的系统解决方案,主要包括扼流圈卫星天线、GNSS数据接收机、MEMS传感器、环境测量单元、防雷区域单元等功能元件的组成、多传感器的系统集成,以及全方位监测的功能实现。按照地灾监测大数据信息平台建设、构建大数据平台关键技术突破及检测数据解算软件功能实现的顺序,完成了GNSS实时监测预警应用网络系统HCmonitor的研发。综合应用多种手段实现地灾监测的功能升级,为解决重大灾害预警提供了新的思路和解决方案,该项成果在甘肃舟曲的灾后重建工程得到应用与推广。     

Improved Priority‐Flood method for depression filling by redundant calculation optimization in local micro‐relief areas

Depression filling is a critical step in distributed hydrological modeling using digital elevation models (DEMs). The traditional Priority‐Flood (PF) approach is widely used due to its relatively high efficiency when dealing with a small‐sized DEM. However, it seems inadequate and inefficient when dealing with large high‐resolution DEMs. In this work, we examined the relationship between the PF algorithm calculation process and the topographical characteristics of depressions, and found significant redundant calculations in the local micro‐relief areas in the conventional PF algorithm. As such calculations require more time when dealing with large DEMs, we thus propose a new variant of the PF algorithm, wherein redundant points and calculations are recognized and eliminated based on the local micro‐relief water‐flow characteristics of the depression‐filling process. In addition, depressions and flatlands were optimally processed by a quick queue to improve the efficiency of the process. The proposed method was applied and validated in eight case areas using the Shuttle Radar Topography Mission digital elevation model (SRTM‐DEM) with 1 arc‐second resolution. These selected areas have different data sizes. A comparative analysis among the proposed method, the Wang and Liu‐based PF, the improved Barnes‐based PF, the improved Zhou‐based PF, and the Planchon and Darboux (P&D) algorithms was conducted to evaluate the accuracy and efficiency of the proposed algorithm. The results showed that the proposed algorithm is 43.2% (maximum) faster than Wang and Liu's variant of the PF method, with an average of 31.8%. In addition, the proposed algorithm achieved similar performance to the improved Zhou‐based PF algorithm, though our algorithm has the advantage of being simpler. The optimal strategies using the proposed algorithm can be employed in various landforms with high efficiency. The proposed method can also achieve good depression filling, even with large amounts of DEM data.     

Sentinel-1A SAR影像及其在矿区地面沉降监测中的应用研究

详细介绍了Sentinel-1A SAR影像数据的基本参数、工作模式、应用领域等。利用2016.11.09—2017.03.09的5景Sentinel-1A C波段SAR影像数据进行矿区地面沉降监测试验。基于SARscape利用双轨D-In SAR技术进行差分干涉处理,得到了研究区地面沉降分布图,直观地再现了研究区在2016.11.09—2017.03.09的沉降分布、沉降量级等。结果表明济宁某矿区在监测期间地面相对稳定,未有大面积和大量级的地面沉降发生,4个干涉对监测到的最大沉降都未超过3 cm。     

湿地资源调查更新若干问题探讨

对古浪县第二次全国湿地资源调查的技术、结果进行了系统分析,探索了研究区内湿地资源调查更新的方法,通过"天空地"协同和"互联网+"的调查更新技术全面更新研究区内湿地资源调查数据库,以全面掌握湿地资源状况。研究结果:1)古浪县第二次湿地资源调查范围内的非湿地类型面积达4 615.35 hm2,占全县第二次湿地调查总面积的59.31%。2)变更后县域内湿地资源总面积4 131.54 hm2,以河流水面、内陆滩涂、水库水面、沟渠为主要用地类型,较第二次调查湿地面积净减少3 650.25 hm2。3)"天空地"协同遥感监测技术为湿地资源变更监测提供高精度多源数据,保证了调查、监测的精度和准确性。     

Application and comparison of two symptom-based eutrophication-assessment methods in Jiaozhou Bay,China

Increasing numbers of approaches to assess eutrophication, such as estuarine trophic status, the Oslo-Paris Commission Common Procedure, and the Water Framework Directive, focus on the symptoms of eutrophication. In China, however, nutrient index methods dominate the assessment of coastal waters. In this study, an integrated method that includes both water quality and ecological response was compared with the Northwest Pacifi c Action Plan (NOWPAP) Common Procedure. Observation data from Jiaozhou Bay, Shandong, China, were used in a comparison of the two methods in a trophic status study. Overall, both clearly revealed a high level of nutrient enrichment in the bay, indicated by high nutrient concentrations. Though the two methods diff ered in their methodological design in the assessment of the ecological eff ects of nutrient enrichment, they have acquired similar results: the integrated method suggested that the status was good, and the NOWPAP Common Procedure suggested that the status was low (indicating that the bay had no serious eutrophication problem). The introduction of fi lter feeders (shellfi sh aquaculture) into the bay on a reasonable scale may have been eff ective in mitigating eutrophic conditions, and perhaps explains the low ecological impacts there. Our results will be useful to ecosystem-based eutrophication management in the bay and in similar areas.     

实时卫星钟差基准精化方法

在基于精密单点定位(PPP)的授时方法中,卫星钟差产品的高精度时间基准至关重要.针对实时卫星钟差产品时间基准不够稳定的问题,本文采用一组具有原子钟外部输入的国际全球卫星导航系统(GNSS)服务(IGS)跟踪站建立了顾及原子钟变化特性的基准精化方法.该方法首先采用阿伦方差对不同的IGS跟踪站外接原子钟进行稳定度分析,挑选出一组稳定度高的原子钟用以精化时间基准.在此基础上,利用阿伦方差分析各台原子钟的噪声参数特征,并确定不同原子钟之间的权比关系.最终,建立时间基准改正量的随机模型,并计算出精化后的时间基准.通过实例验证表明:与IGS事后精密钟差产品定义的时间基准比较,改正后的实时钟差基准单天内的标准差(STD)优于0.1 ns,相比于改正前最高提升了93%.同时,基准改正后的天内万秒稳达到10^-15量级,实现了一个量级的提高.此外,通过相对钟差精度的分析,表明钟差基准修正不影响PPP的定位精度.