三疣梭子蟹全人工工厂化育苗技术

2000年笔者在日照市水产研究所利用1450m^3水进行梭子蟹全人工工厂化育苗生产，共出Ⅱ-Ⅲ期幼蟹512．7万只，创造了出苗量为3500只／m^3，总产值为150多万元的直接经济效益，同时对幼体培育的密度进行了初步试验，结果表明：从Z1至M期幼体培育密度10－15万／m^3为宜，M期以后密度以0．8－1．5万／m^3为宜。     

Fatigue Behavior of Plain Concrete Under Biaxial Compression: Experiments and Theoretical Model

The effects of different lateral confinement stress on the fatigue behavior of and cumulative damage to plain concrete are investigated experimentally. Eighty 100 mm x 100 mm x 100 mm specimens of ordinary strength concrete are tested under constant- or variable-amplitude fatigue loading and lateral confinement pressure in two orthogonal directions. A fatigue equation is developed by modifying the classical Aas-Jakobsen S-N equation for taking into account the effect of the confined stress on fatigue strength of plain concrete. The results of variable-amplitude fatigue tests indicate that the linear damage theory proposed by Palmgren and Miner is unreasonable in the biaxial stress state. A nonlinear cumulative damage model that could model the effects of the magnitude and sequence of variable-amplitude fatigue loading and lateral confinement pressure is proposed on the basis of the evolution laws of the residual strains in the longitudinal direction during fatigue tests. The residual fatigue life predic     

An Experimental Study of Several Special Issues Regarding the Ice Force on Conical Structures

The effect of the cone spacing of a conieal structure on the ice force is studied by model experiments. The ice force reduction coefficient presented in this paper expresses the relationship between the ice force and the arrangement of cones. The experiments prove that the mode of the ice failure before the boundary of upward-downward bending cone (UDBC) is crushing. A conclusion can also be drawn from the experiments that the ice force on the boundary of UDBC is by far less than that on a vertical pile with the same diameter. Moreover, the ice force frequencies on upright and inve-rted cones are obtained under the field condition of the platform JZ20-2, respectively. The results show that the alterna-tion of the ice force on UDBC can hardly induce resonance of platform JZ20-2.     

Model Test Study of Dynamic Ice Force on Compliant Conical Structures

To study ice-induced vibration of a compliant conical structure,a series of model tests were performed from 2004 to 2005.In the tests,the ice sheet before the compliant conical structure was found to fail in two-time breaking.From 2005 to 2006,this type of ice failure was studied through more groups of tests.The tests show that two-time breaking is the typical failure of ice before steep conical structures,and is controlled by other factors at the same time,such as ice speed and the angle of the cone.     

“数字海底”系统建模中的关键技术研究

基于“数字海底”系统的理论基础研究和在海底科学应用中的具体实践,提出了“数字海底”的涵义、系统组成和体系结构,对空间数据管理技术、基于XML的WebGIS空间数据表达技术、复杂海底三维建模技术、多源信息的一体化集成显示技术、虚拟现实技术等“数字海底”系统建模中的关键技术进行了探讨,为从事海底资源研究和开发的人员提供各个层次的参考或帮助.     

Analysis of Dynamic Characteristics of Submarine Free Spanning Pipelines by Complex Damping Method

Considering the effect of the internal flowing fluid and the external marine environmental condition, the differential equation for the vortex-induced vibration (V1V) of the free spanning pipeline is derived and is discretized by the Hermit interpolation function. The free vibration equation with the damping term is solved by the complex damping method for the natural frequency, and then the effect of fluid damping on the natural frequency of the free spanning pipeline is analyzed.The results show that fluid damping has a significant influence on the damped natural frequency of the free spanning pipeline in the lock-in state, while it has little influence when the pipeline is out of the lock-in state. In the meantime,the change of the free span length has the same effect on the damped natural frequency and the undamped natural frequency.     

新建水坝对下游已建水工建筑物的影响分析

分析了新建水坝及社会经济发展因素对坝下游水流及泥沙的影响，用一个简易河床模型来说明新建大坝后下游河床的演变规律，通过坝下游河床一般演变分析来阐述局部冲刷对已建水工建筑物基础的影响及对已建水工建筑基础的防护措施。     

浮游植物叶绿素a含量简易测定方法的比较

用乙醇、DMF(N,N-二甲基甲酰胺)、丙酮3种不同有机溶剂分别萃取苏州环城河水样中的浮游植物细胞叶绿素a,在分光光度计上测定其叶绿素a含量,试验数据经方差分析、Q检验,结果显示,乙醇法、DMF法的叶绿素a萃取率极显著高于丙酮(F=54.20＞F0.01(2,18)(6.01)).另用这3种萃取方法分别测定无常蓝纤维藻(Dactylococcopsis irregularis )纯培养液叶绿素a含量,通过直线回归,同样证明乙醇法、DMF法重复率较高,稳定性好,萃取率高.从安全、简便、重复性高等方面综合考虑,认为用乙醇萃取法是目前水域环境中浮游植物叶绿素a简易测定的最佳方法.     

应用遗传神经网格方法分析赤潮监测数据

简要介绍了遗传神经网络方法的原理 ,探讨了应用遗传神经网络方法研究辽东湾海域丹麦细柱藻 (Leptocylindrusdanicus)赤潮与其环境因子之间的关系 ,计算了各环境因子对丹麦细柱藻赤潮的贡献。结果表明 ,温度、盐度、DIN的变化对研究海域丹麦细柱藻种群密度的增长有比较重要的影响 ,DIN是营养限制因子。遗传神经网络是分析赤潮监测数据的有效方法 ,值得进一步探索     

病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。