水生细菌数量与菌体大小测定技术的进展

水生细菌的数量与菌体大小是海洋微生物研究的关键参数，随着技术的进步，测定这些参数的方法有了很大的发展．本文介绍了其测定技术的发展历程，并对已有的测定方法进行了介绍、归纳和评述，认为流式细胞仪测定法是测定细菌数量与菌体大小较为合适的方法。     

Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome

An open reading frame (lcn61) of iymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector.Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.     

Phytoplankton pigment patterns and community composition in the northern South China Sea during winter

Phytoplankton pigment patterns and community composition were investigated in the northern South China Sea using high-performance liquid chromatography and the CHEMTAX software from February 11 to 23, 2009. We recognized four different vertical distribution patterns of pigments: chlorophyll a (Chl a)-like type, divinyl chlorophyll a (DV Chl a) type, even distribution type, and surface type. The average value of ratios of accessory photo-protective pigments (APP) to accessory photo-synthetic pigments was 0.8...     

Screen and effect analysis of immunostimulants for sea cucumber,Apostichopus japonicus

Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal's coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides (P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day 1 and day 2 after the injection (P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection (P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.     

Vertical properties of nutrients and oxygen under tempeuature-salinity structure of the Bering Basin in July 1999

The China R/V Xuelong went on the first Arctic scientific cruise, and we obtained 271 hydro-chemical samples from 22 deep-sea stations in the Bering Basin in late July, 1999. Here we describe vertical properties of silicate [Si] , dissolved inorganic nitrogen [DIN] or [N] (nitrate plus ammonium plus nitrite) , phosphate [P] and oxygen [ O2 ] in seawater under potential temperature-salinity structure. The seasonal stratification in the summer and the water exchanges of the North Pacific Ocean over the Bering Basin resulted in that the four layers of vertical structure with two thermoclines may be found. Vertical [Si] and [N] and [P] profiles show that the nutrients are consumed mainly in ≤50 m and the order of deficient nutrients is [Si] the first, [N] the second and [P] the third. The [N] and [P] increase with depth downward to about 500 m and then both decrease, but the [Si] increases from 150 m to 2000 m or the bottom. In ≥ 150 m the [ O2 ] decreases, which is related with both [P] and [N] increasin     

A VERIFICATION ON DIVISION OF HYDROCLIMATIC AREA IN CHINA SEAS USING DIGITAL CHARACTERISTIC OF FREQUENCY DISTRIBUTION

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东库克海峡腔吻鳕(Macruronus novaezelandiae)卵及稚鱼与水文状况的关系

1987年9月,我们进行了一次详细的调查,确定了东库克海峡(Cook Stait)中腔吻鳕的卵及稚鱼分布与水文状况的关系。由于地方性风的原因,当地的水文结构非常复杂。南塔拉纳基湾(Taranaki Bight)水占据了库克海峡北部和中部的大部分水域。克劳迪湾(Cloudy Bay)沿岸水被河水冲淡,并由于库克海峡峡谷上升流进入克利福德湾(Clifford Bay)和开普.坎贝尔(Cape Campbell)南部沿岸,使克劳迪湾的沿岸水向近岸呈羽状伸展。东开普流水体出现在库克海峡峡谷水深200m附近和陆架外缘。开普.帕利瑟(Cape Palliser)南部的深层混合说明,该地区有一股反气旋涡旋。叶绿素α浓度与混合层深度有关,它在垂直分层的表面水体中最高。腔吻鳕卵在库克海峡峡谷内最多,该地区是这种鱼类著名的产卵场。小的腔吻鳕稚鱼(2.0～3.9mm)数在卵高密度集区和开普。坎贝尔附近最多,而较大的稚鱼(>10mm)在近岸区最多。根据刚孵化出来的稚鱼的分布情况来看,腔吻鳕卵随着当地的上升流进入开普。坎贝尔的近岸地区。     

Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus)

Lymphocystis disease,caused by the lymphocystis disease virus (LCDV),is a significant worldwide problem in fish industry causing substantial economic losses.In this study,we aimed to develop the DNA vaccine against LCDV,using DNA vaccination technology.We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate.The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line.The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also ana...