Identification of dissolved and particulate carbonyl compounds produced by marine harmful algal bloom species

Carbonyl compounds, especially polyunsaturated aldehydes (PUAs), are increasingly reported as teratogenic to grazers and deleterious to phytoplankton. While PUAs have been considered to be mainly produced by diatoms after cell wounding, little is known about whether microalgae other than diatoms, particularly harmful algal bloom (HAB)-forming species, produce carbonyl compounds. In this study, we analyzed the carbonyl compounds from eight common HAB-forming species ( Akashiwo sanguinea , Karenia mikimotoi , Karlodinium venefi cum , Margalefi dinium polykrikoides , Prorocentrum donghaiense , P . minimum , Scrippsiella trochoidea , and Heterosigma akashiwo ) using gas chromatography-mass spectrometry in full scan and selected ion monitoring (SIM) modes. Our results show a ubiquitous presence of carbonyl compounds in both dissolved and particulate forms in all species we examined. In the full scan mode, 133 chromatographic peaks were detected from 51 samples altogether. Both the varieties of carbonyl compounds and their quantities were algal species dependent, although most of the carbonyl compounds could not be fully identifi ed according to the mass spectral database only due to the unavailability of enough standards for all analytes. Aided with nine standards in SIM mode, we further identifi ed and quantifi ed all nine aldehydes (2-methyl-2-pentenal, trans-2-nonenal, cis-6-nonenal, 2,6-dimethyl-5-heptenal, trans-2-hexenal, trans-2- decenal, 2,4-heptadienal, trans-trans-2,4-octadienal, and trans-trans-2,4-decadienal). Four of these nine aldehydes were detected in particulate form, which confi rmed that trans-2-nonenal could be produced by K . mikimotoi , Karl . venefi cum , P . donghaiense , P . minimum , S . trochoidea , and H . akashiwo , 2,4-heptadienal and trans-trans-2,4-decadienal by A . sanguinea , M . polykrikoides , and S . trochoidea , and trans, trans-2,4- octadienal by S . trochoidea , respectively. We proved that some dinofl agellate and raphidophyte species could contribute to the pool of carbonyl compounds including PUAs in a marine ecosystem. Some carbonyl compounds, particularly those in high cell quota and/or dissolved concentration, may be related to fi shkilling or allelopathy which needs further identifi cation and quantifi cation.     

Responding to Global Warming: Mitigation Policies and Actions of Stakeholders in China’s Tourism Industry

China’s tourism industry has witnessed rapid progress in recent years, and is now an important part of global tourism in dealing with climate change. Within a framework of Pressure-State-Response (PSR), this paper focuses on the emission reduction pressure, carbon emission status, and responses of stakeholders in China’s tourism industry. Findings include: 1) The central government’s strategy and rapid growth of the industry scale exert rising pressure on China’s tourism to reduce carbon emissions. 2) Carbon emissions of China's tourism account for 13%-14.6% of global tourism, and about 3% of China’s emissions overall. Chinese tourists’ per capita carbon emission is lower than half of the global level. 3) The Chinese government attaches great importance to energy-saving and carbon emission reduction. In the tourism industry, documents, standards and other regulative measures have been issued to ensure that business practitioners set up green operational and managerial systems. In the field of tourism transportation, China's high-speed rail, new energy vehicles, and urban shared bicycles, have developed very rapidly in recent years, and they have effectively reduced the carbon emissions in traveling. Furthermore, this paper finds that Chinese tourists already have awareness and willingness for low-carbon tourism.     

南海东北部内潮的模态结构和传播

The evolution of energy, energy flux and modal structure of the internal tides(ITs) in the northeastern South China Sea is examined using the measurements at two moorings along a cross-slope section from the deep continental slope to the shallow continental shelf. The energy of both diurnal and semidiurnal ITs clearly shows a～14-day spring-neap cycle, but their phases lag that of barotropic tides, indicating that ITs are not generated on the continental slope. Observations of internal tidal energy flux suggest that they may be generated at the Luzon Strait and propagate west-northwest to the continental slope in the northwestern SCS. Because the continental slope is critical-supercritical with respect to diurnal ITs, about 4.6 kJ/m~2 of the incident energy and 8.7 kW/m of energy flux of diurnal ITs are reduced from the continental slope to the continental shelf. In contrast, the semidiurnal internal tides enter the shelf because of the sub-critical topography with respect to semidiurnal ITs.From the continental slope to the shelf, the vertical structure of diurnal ITs shows significant variation, with dominant Mode 1 on the deep slope and dominant higher modes on the shelf. On the contrary, the vertical structure of the semidiurnal ITs is stable, with dominant Mode 1.     

海洋微藻多糖微波提取法研究

选取自养小球藻(Chlorella autotrophica)为实验材料,采用微波法提取多糖,以温度、时间、pH以及微波功率4个因子建立正交实验,考察单因素影响情况。实验结果表明,在温度70℃、时间30min、pH为7、功率为600W条件下粗多糖的产率最高,为291.13mg/g(粗多糖/干重),远高于传统热水浸提法所得粗多糖产率(78.52mg/g,粗多糖/干重),且所得粗多糖与热水浸提法获得的粗多糖具有相似的红外光谱。说明,在一定的实验条件下,与热水浸提法相比,微波提取法不仅不会破坏多糖的结构,还有耗时短、有较高的多糖产率的优势。     

渤、黄海北部海冰年代时空变化特征分析

本文取上世纪后50年至2003年度冬季渤海地区气温作为与渤海冬季冰情有关的气候指标。分析得出,渤海地区冬季气温随着年代呈上升趋势,特别是80年代中期至本世纪初尤为明显。受其影响,渤海冬季的冰情时空分布状况发生了较大变化。其特点是:初冰日较多年平均值推后、终冰日较多年平均值提前,冰期较多年平均值偏短,冰厚较多年平均值偏薄,结冰范围(沿岸固定冰宽度和浮冰外缘线)较多年平均值偏小,甚至有些海区已多年未曾见到海冰了。     

互花米草入侵对河口盐沼湿地食物网的影响

盐沼湿地因其在河口及沿岸水域生态系统中的重要地位而被普遍关注,近年来互花米草在长江口乃至整个东南沿海地区的入侵及迅速蔓延更是引起了广泛重视。由于对维系本区域湿地生态系统运转的食物网结构的了解仍很有限,从而难以准确评估互花米草入侵后对原有生态系统的影响。本研究通过基于碳、氮稳定同位素测定结果的IsoSource软件估算和聚类分析,对长江口盐沼湿地内不同初级生产者的相对营养贡献大小进行了初步评估。结果显示在该湿地食物网中,底栖微藻和本土陆源C3植物是主要的营养来源。入侵C4植物互花米草的营养贡献不显著,在本次研究中仅有堇拟沼螺这一腹足类消费者在夏季较为确定地表现出对源自互花米草的富δ13C的有机质的摄取。随着互花米草的迅速扩散,其在长江口湿地的有机质产出中所占比例还将不断增加,这将可能从营养基础的层面自下而上对该食物网的结构和功能造成影响。     

南海北部陆坡海域内波谱特征

本文对1998年南海季风实验中定点观测的声学多普勒流速剖面仪（Acoustic Doppler Current Profiler,ADCP）海流数据进行频谱分析,结果表明南海北部地区内波之间的非线性作用的强弱影响着该海域的内波谱特征。在季节性温跃层（水深22m和58m）中,由于存在着强的非线性作用,内波带的谱斜率趋于σ^-1和σ^-2之间,而且较高能量的潮谐波频率的峰值随着频率呈现少见的一。递减。在季节性温跃层（水深130m）以下,内波非线性相互作用较弱,观测的内波带谱特征与标准Garrett-Munk内波谱相似,均显示了σ^-2的谱斜率。     

Water, organic matter, nitrogen and phosphorus contents in sediment of a large-scale mariculture area in the Zhelin Bay of eastern Guangdong Province, China

The Zhelin Bay is one of the most important bays for large-scale mariculture in Guangdong Province, China. Owing to the increasing human population and the expanding mariculture in the last two decades, the ecological environment has greatly changed with frequent harmful algal blooms. A monthly survey of water content, organic matter （TOM）, and various forms of nitrogen and phosphorous in sediment from July 2002 to July 2003 in the bay was conducted. The results showed that the water content was correlated significantly with TOM and various forms of nitrogen and phosphorus and can be used as proxy for quick and rough estimate of these factors in the future surveys. TOM was also correlated significantly with various forms of nitrogen and phosphorus, indicating that it was one of the key factors affecting the concentrations and distributions of nitrogen and phosphorus in the investigated waters. Average total Kjeldhal nitrogen （TkN） content was（ 1 113.1 ± 382.5）μg/g and average total phosphorus （TP） content was（567.2± 223.3）μg/g, and both were much higher than those of similar estuaries in China and elsewhere. Average nitrogen and phosphorus tended to be higher inside than outside the bay, higher at aquaculture than non-aquaculture areas, and higher at fish-cage culture than oyster culture areas, suggesting that large-scale mariculture inside the bay played an important role in the eutrophication of the Zhelin Bay. Various forms of nitrogen and phosphorus concentrations were higher during the warm season （July--September）, which was due to the increased decomposition and concentration of organic matter resulted from the fast growth and high mortality of the cultured species. Compared with July 2002, TkN and TP contents were much higher in July 2003, in consonance with the eutrophication of the Zhelin Bay. Because exchangeable phosphorus （Ex-P）, iron-bounded phos- phorus （Fe-P） and organic phosphorus （OP） combined accounted for 34.3% of the TP and authigenic phosphorus （Au-P     

东海陆架两期沙脊的时空对比东海陆架两期沙脊的时空对比

基于高分辨率的单道地震和多波束测深数据,识别并对比了东海陆架中部同一海区相距20余万年的层U14和层U2两期沙脊群,其中层U14期沙脊属于埋藏沙脊,位于东海海底以下90 m深处,推测属于距今320~200 ka的海侵体系域(TST),沙脊顶界面是该期海侵的最大洪泛面(MFS);层U2期沙脊位于东海陆架,属于衰退沙脊,系末次盛冰期(LGM)以来的TST,顶界面是LGM以来的MFS。尽管两期沙脊形成年代相距20余万年,地层层位相距近90 m,但是沙脊群总体走向一致,表明距今2×105 a以来东海陆架潮波基本格局稳定。从层U2期可识别出4个亚期沙脊,通过多波束海底地形图可识别出4组走向的沙脊,多亚期、多走向沙脊是LGM以来海平面阶梯状波动在海底地形演变过程中的响应证据。     

南海东北部陆缘区新近纪早期反转构造特征及其动力学意义

通过深入分析"九七三"项目所获取的高分辨率多道地震剖面,结合区域钻井资料,探讨了新生代以来南海东北部陆缘所历经的新生代早期张裂、中新世晚期阶段性挤压及上新世以来的稳定热沉降等复杂构造运动;张裂背景下的南海东北部陆缘在中新统中部、下部出现的背斜、逆断层等典型压性反转构造,凸现了南海东北部陆缘在晚中新世发生的阶段性挤压构造运动;分析表明,此构造活动是东沙运动在台西南海域的表现,其动力来源同菲律宾海板块的北西向运动有关。